A coumarin-based compound（1） was designed and synthesized as a new turn-on fluorescent probe for the detection of cysteine. The probe exhibited higher selectivity towards the target molecule over other thiol and ami...A coumarin-based compound（1） was designed and synthesized as a new turn-on fluorescent probe for the detection of cysteine. The probe exhibited higher selectivity towards the target molecule over other thiol and amino acids at pH 7.2 in aqueous media CH_3CN-HEPES（0.02 mol/L, pH 7.2, 1：9, v/v）. The reaction mechanism is attributed to the cysteine-induced S_NAr substitution-rearrangement reaction. Remarkable enhancement of up to 20-fold in fluorescence intensity was achieved in the detection of cysteine. When applied for the fluorescence imaging of cysteine, the compound 1 emitted a green fluorescence in Hi5 cell cytoplasm. The in vivo imaging of Caenorhabditis elegans had further confirmed the cysteine detection by compound 1.展开更多
A rhodamine-based sensor has been developed for the detection of mercuric ions. The colorimetric and fluorescence responses, allowing naked-eye detections, are based on Hg^2＋-induced opening of the rhodamine spirocyc...A rhodamine-based sensor has been developed for the detection of mercuric ions. The colorimetric and fluorescence responses, allowing naked-eye detections, are based on Hg^2＋-induced opening of the rhodamine spirocycle. Among all the testes ions, only Hg^2＋generated a significant fluorescence enhancement of up to 300-fold, with a bright yellow–green emission. This sensor was a low toxic compound, and was successfully applied in the in vivo imaging of Hg^2＋in Spill 2 cells and C. elegans. This approach provides a sensitive and accurate method for the estimation of Hg^2＋in environmental, tobacco and biological applications.展开更多
A rhodamine-based sensor（1） has been developed for the detection of chromium ions.Cr-（3＋）-induced opening of the rhodamine spirocycle in sensor（1） led to the distinct colorimetric and fluorescence responses.Amo...A rhodamine-based sensor（1） has been developed for the detection of chromium ions.Cr-（3＋）-induced opening of the rhodamine spirocycle in sensor（1） led to the distinct colorimetric and fluorescence responses.Among all the tested ions,only Cr-（3＋） generated a significant fluorescence enhancement of up to13-fold,which indicated the high selectivity of 1.Sensor（1） was successfully applied in the in vivo fluorescence imaging of Cr-（3＋） in C.elegans.The results provided solid evidences for the future estimation of Cr-（3＋） in environmental applications and tobacco samples.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21462050 and 21672185)the Foundation of the Department of Science and Technology of Yunnan Province of China (Nos. 2013HB062, 2014HB008, 2016FB020,2016FB023)the Program for Excellent Youth Talents, Yunnan University (No. XT412003)
文摘A coumarin-based compound（1） was designed and synthesized as a new turn-on fluorescent probe for the detection of cysteine. The probe exhibited higher selectivity towards the target molecule over other thiol and amino acids at pH 7.2 in aqueous media CH_3CN-HEPES（0.02 mol/L, pH 7.2, 1：9, v/v）. The reaction mechanism is attributed to the cysteine-induced S_NAr substitution-rearrangement reaction. Remarkable enhancement of up to 20-fold in fluorescence intensity was achieved in the detection of cysteine. When applied for the fluorescence imaging of cysteine, the compound 1 emitted a green fluorescence in Hi5 cell cytoplasm. The in vivo imaging of Caenorhabditis elegans had further confirmed the cysteine detection by compound 1.
基金supported by the fund of China Tobacco Yunnan Industrial Co. (No. 2015JC05)Foundation of the Department of Science and Technology of Yunnan Province of China (Nos. 2013HB062, 2014HB008)Training Project (No. XT412003) of Yunnan University
文摘A rhodamine-based sensor has been developed for the detection of mercuric ions. The colorimetric and fluorescence responses, allowing naked-eye detections, are based on Hg^2＋-induced opening of the rhodamine spirocycle. Among all the testes ions, only Hg^2＋generated a significant fluorescence enhancement of up to 300-fold, with a bright yellow–green emission. This sensor was a low toxic compound, and was successfully applied in the in vivo imaging of Hg^2＋in Spill 2 cells and C. elegans. This approach provides a sensitive and accurate method for the estimation of Hg^2＋in environmental, tobacco and biological applications.
基金supported by fund of China Tobacco Yunnan Industrial Co.(No.2015JC05)the Foundation of the Department of Science and Technology of Yunnan Province of China(Nos.2013HB062,2014HB008,2016FB020)the Program for Excellent Youth Talents of Yunnan University(No.XT412003)
文摘A rhodamine-based sensor（1） has been developed for the detection of chromium ions.Cr-（3＋）-induced opening of the rhodamine spirocycle in sensor（1） led to the distinct colorimetric and fluorescence responses.Among all the tested ions,only Cr-（3＋） generated a significant fluorescence enhancement of up to13-fold,which indicated the high selectivity of 1.Sensor（1） was successfully applied in the in vivo fluorescence imaging of Cr-（3＋） in C.elegans.The results provided solid evidences for the future estimation of Cr-（3＋） in environmental applications and tobacco samples.
