Bacterial cellulose film containing Sarapee （Mammea siamensis） flower extract to against Salmonella typhimurium TISTR 292 was studied. Crude ethanol extract of flower from Sarapee was passed through the column chrom...Bacterial cellulose film containing Sarapee （Mammea siamensis） flower extract to against Salmonella typhimurium TISTR 292 was studied. Crude ethanol extract of flower from Sarapee was passed through the column chromatography in order to separate the flavonoid. The flavonoid content was about 7.97 mg/mL. Bacterial cellulose film produced by ,4cetobacter xylinum TISTR 975 in coconut juice and subsequently to application of a containing different concentration of flavonoid for 12 h and followed by freeze dry The bacterial cellulose film containing Sarapee extract using disc diffusion assay was tested for antimicrobial activity. The result showed that bacterial cellulose film containing 1.2 mg/mL of flavonoid was highly against to S. typhimurium TISTR 292 and the inhibition zone was about 31.07 mm. Investigations of the effect of coating time on incorporation of flavonoid into cellulose film showed that long coating time （12 h） had the inhibition zone closely to short coating time （8 h） with the value of 29.60-30.13 mm. Stored the bacterial cellulose film at room temperature for 7 and 14 days found that the ability to be against S. typhimurium TISTR 292 decreased after 14 days and the inhibition zone was about 22.63 mm. The percentage of ability was remaining about 75.4. This study suggests that the bacterial cellulose film has a potential for use as antimicrobial in food applications.展开更多
文摘Bacterial cellulose film containing Sarapee （Mammea siamensis） flower extract to against Salmonella typhimurium TISTR 292 was studied. Crude ethanol extract of flower from Sarapee was passed through the column chromatography in order to separate the flavonoid. The flavonoid content was about 7.97 mg/mL. Bacterial cellulose film produced by ,4cetobacter xylinum TISTR 975 in coconut juice and subsequently to application of a containing different concentration of flavonoid for 12 h and followed by freeze dry The bacterial cellulose film containing Sarapee extract using disc diffusion assay was tested for antimicrobial activity. The result showed that bacterial cellulose film containing 1.2 mg/mL of flavonoid was highly against to S. typhimurium TISTR 292 and the inhibition zone was about 31.07 mm. Investigations of the effect of coating time on incorporation of flavonoid into cellulose film showed that long coating time （12 h） had the inhibition zone closely to short coating time （8 h） with the value of 29.60-30.13 mm. Stored the bacterial cellulose film at room temperature for 7 and 14 days found that the ability to be against S. typhimurium TISTR 292 decreased after 14 days and the inhibition zone was about 22.63 mm. The percentage of ability was remaining about 75.4. This study suggests that the bacterial cellulose film has a potential for use as antimicrobial in food applications.
