Wool keratin has broad application prospects in biomedical materials, tissue engineering, and other fields. To obtain porous wool keratin membrane with good performance, and lay the foundation for its application in b...Wool keratin has broad application prospects in biomedical materials, tissue engineering, and other fields. To obtain porous wool keratin membrane with good performance, and lay the foundation for its application in biological fields in the future, wool keratin aqueous solution and sponge were prepared and their characters were discussed. Wool fiber was dissolved with a mixture of urea, β-mercapitoethanol, sodium dodecyl sulfate(SDS) and water, and stable keratin aqueous solution was prepared through filtration and dialysis. Dissolution rate under different conditions was discussed. The results show that dissolution rate increases with the increase of dissolving temperature, reduction β-mercaptoethanol and assistant agent urea concentration and the addition of SDS. SDS polyacrylamide gel electrophoresis (SDS PAGE) shows the major molecular weight of the aqueous keratin solution is between 44300 u and 66400 u. Amino acid analysis shows the cysteine acid content of the keratin solution decreases obviously. Wool keratin sponge was made from the keratin solution with freeze drying method and its structure and characters were studied with scanning electron microscopy(SEM) and Fourier transform infrared spectrometer (FTIR). The results indicate that keratin sponge has evident pores structure, and the conformation of the sponge is β-sheet.展开更多
In this paper, we discussed the relationship of filter efficiency and pressure drop with the porosity, fiber diameter and filter thickness by Geodict. We found that filter efficiency will increase when filter porosity...In this paper, we discussed the relationship of filter efficiency and pressure drop with the porosity, fiber diameter and filter thickness by Geodict. We found that filter efficiency will increase when filter porosity and fiber diameter decreasing or filter thickness increasing. And the pressure drop has a linear relationship with filter thickness and non-linear relationship with filter porosity and fiber diameter. We also compared the simulation results with the real test results by TSI 3160. Although there are some differences, I think Geodict can be used to predict filter efficiency and pressure drop.展开更多
基金Natural Science Foundation of Hubei Province,China ( No. 2008CDA059)
文摘Wool keratin has broad application prospects in biomedical materials, tissue engineering, and other fields. To obtain porous wool keratin membrane with good performance, and lay the foundation for its application in biological fields in the future, wool keratin aqueous solution and sponge were prepared and their characters were discussed. Wool fiber was dissolved with a mixture of urea, β-mercapitoethanol, sodium dodecyl sulfate(SDS) and water, and stable keratin aqueous solution was prepared through filtration and dialysis. Dissolution rate under different conditions was discussed. The results show that dissolution rate increases with the increase of dissolving temperature, reduction β-mercaptoethanol and assistant agent urea concentration and the addition of SDS. SDS polyacrylamide gel electrophoresis (SDS PAGE) shows the major molecular weight of the aqueous keratin solution is between 44300 u and 66400 u. Amino acid analysis shows the cysteine acid content of the keratin solution decreases obviously. Wool keratin sponge was made from the keratin solution with freeze drying method and its structure and characters were studied with scanning electron microscopy(SEM) and Fourier transform infrared spectrometer (FTIR). The results indicate that keratin sponge has evident pores structure, and the conformation of the sponge is β-sheet.
基金Supported by SRC/ERC Programof MOST/KOSEF (R11 -2005 -065) ,Soongsil University
文摘In this paper, we discussed the relationship of filter efficiency and pressure drop with the porosity, fiber diameter and filter thickness by Geodict. We found that filter efficiency will increase when filter porosity and fiber diameter decreasing or filter thickness increasing. And the pressure drop has a linear relationship with filter thickness and non-linear relationship with filter porosity and fiber diameter. We also compared the simulation results with the real test results by TSI 3160. Although there are some differences, I think Geodict can be used to predict filter efficiency and pressure drop.
