The effect of Survivin antioligonucleotide on the apoptosis of Xuanwei lung adenocarcinoma cell

Objective:The aim of the study was to investigate the effects of Survivin antioligonucleotide on the proliferation,apoptosis and cell cycle of Xuanwei lung adenocarcinoma cell XWLC-05.Methods:Specific targeting Survivin ASODN was composed firstly.XWLC-05 would be divided into 4 groups:Group Sham(blank),Group Lip(simple liposome),Group Lip-SODN(transfected sense oligonucleotide) and Group Lip-ASODN(transfected ASODN).All groups had been transfected under the same condition for 48 hours.Then West blotting was used to check the expression of Survivin in cells from different groups and flow cytometer was used to find out the apoptosis rate of cells in different groups.Results:The expression of XWLC-05 Survivin in Group Lip-ASODN decreased obviously and apoptosis rate was significantly higher than that of other groups(P < 0.05).Conclusion:XWLC-05 transfected by Survivin ASODN could down regulate the expression of Survivin and lower expression of Survivin might lead to the apoptosis of XWLC-05 and restrain the proliferation of cancer cells.

The expression of Survivin in lung adenocarcinoma:a comparative study in Xuanwei and Kunming

Objective: The aim of our study was to investigate the expression of Survivin in lung adenocarcinoma of Xuanwei and Kunming patients. Methods: Twenty-five specimens of Xuanwei patients and 28 specimens of Kunming patients were observed and analyzed. The Survivin expression was detected by immunohistochemistry. The results were quantitatively analyzed by image analysis system. Results: There were significant differences in Survivin expression (P < 0.01) between Xuanwei patients and Kunming patients. Conclusion: Survivin expression in lung adenocarcinoma of Xuanwei patients was significantly higher than that of Kunming patients. The pathogenesis of lung adenocarcinoma might be different between Xuanwei patients and Kunming patients. High Survivin expression might be one of the reasons to explain high incidence of lung cancer in Xuanwei.

Isolated Right Pulmonary Agenesis in a 2-Month-Old Infant in the Pediatric Ward of Hospital of Mali

Introduction: Pulmonary agenesis is a rare congenital abnormality defined by the absence of parenchyma, bronchi and pulmonary vessels, frequently associated with other malformations. We describe a case of isolated right pulmonary agenesis diagnosed in the pediatric ward of the Mali Hospital. Clinical case: He was a month-old baby boy, born at term by cesarean section of a well-followed pregnancy. He was not resuscitated. He developed dyspnea after a cold. An emergency chest X-ray revealed a right opaque lung. He was referred to the hospital for the exploration of this opacity. At the entrance, it weighed 3910 g, size: 54 cm and the temperature was 36.9°C. He had a polypnea, an intercostal print. The sounds of the heart were diverted to the right. The rest of the clinical examination was without much particularity. The chest CT scan confirmed the right pulmonary agenesis. Abdominal and cardiac ultrasound was normal. A healing includes oxygenotherapy, serum glucose 10% minding 100 ml/ Kg/day and amoxicillin minding 100 mg/ Kg/day at 3 times by oral way. After 24 hours of treatment, we observed an improvement in dyspnea. On D2, he was weaned off oxygen and resumed feeding. Conclusion: Pulmonary agenesis is a rare congenital anomaly that is frequently associated with other malformations, thus making its prognosis poor. The search for malformative abnormalities should be systematic in right pulmonary agenesis.