Objective To examine the effect of the root extract on the growth factor during early pregnancy from day 2 to day 6 of gestation. Methods A purified form of root extract of Polygonum hydropiper was obtained by Thin La...Objective To examine the effect of the root extract on the growth factor during early pregnancy from day 2 to day 6 of gestation. Methods A purified form of root extract of Polygonum hydropiper was obtained by Thin Layer Chromatography （TLC）. Localization of transforming growth factor （TGF）- β1 was studied by immunohistochemistry and the expression of the mRNA of TGF- β1 was studied by Reverse transcriptase Polymerase Chain Reaction （RT-PCR） in uterine tissue from day 2 to day 6 of gestation. Results The TLC fraction treated in a dose of 5 mg/kg body weight per day showed decreased the number of implantation sites. The expression of TGF- β1 from day 2 to day 6 of gestation was altered by the TLCfraction and suppression of the mRNA transcript of the TGF- β1 on day 6 of gestation during implantation. Conclusion The root extract of Polygonum hydropiper in a dose of 5 mg/kg body weight per day decreased the expression of TGF- β1 in the implantation sites of rat uterus.展开更多
文摘Objective To examine the effect of the root extract on the growth factor during early pregnancy from day 2 to day 6 of gestation. Methods A purified form of root extract of Polygonum hydropiper was obtained by Thin Layer Chromatography （TLC）. Localization of transforming growth factor （TGF）- β1 was studied by immunohistochemistry and the expression of the mRNA of TGF- β1 was studied by Reverse transcriptase Polymerase Chain Reaction （RT-PCR） in uterine tissue from day 2 to day 6 of gestation. Results The TLC fraction treated in a dose of 5 mg/kg body weight per day showed decreased the number of implantation sites. The expression of TGF- β1 from day 2 to day 6 of gestation was altered by the TLCfraction and suppression of the mRNA transcript of the TGF- β1 on day 6 of gestation during implantation. Conclusion The root extract of Polygonum hydropiper in a dose of 5 mg/kg body weight per day decreased the expression of TGF- β1 in the implantation sites of rat uterus.
