Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precult...Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co cultivated with Agrobacterium tumefaciens , LBA4404, which contains plasmid vector pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4 D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.展开更多
This paper reports the influences of the herbicide butachlor( n butoxymethl chloro 2', 6' diethylacetnilide) on microbial respiration, nitrogen fixation and nitrification, and on the activities of dehyd...This paper reports the influences of the herbicide butachlor( n butoxymethl chloro 2', 6' diethylacetnilide) on microbial respiration, nitrogen fixation and nitrification, and on the activities of dehydrogenase and hydrogen peroxidase in paddy soil. The results showed that after application of butachlor with concentrations of 5.5 μg/g dried soil, 11.0 μg/g dried soil and 22.0 μg/g dried soil, the application of butachlor enhanced the activity of dehydrogenase at increasing concentrations. The soil dehydrogenase showed the highest activity on the 16th day after application of 22.0 μg/g dried soil of butachlor. The hydrogen peroxidase could be stimulated by butachlor. The soil respiration was depressed within a period from several days to more than 20 days, depending on concentrations of butachlor applied. Both the nitrogen fixation and nitrification were stimulated in the beginning but reduced greatly afterwards in paddy soil.展开更多
Arsenic （As） is one of the most important elemental pollutants in groundwater and drinking water because it causes health problem of arsenicosis after consumption of drinking arsenic-rich water more than 5-10 years....Arsenic （As） is one of the most important elemental pollutants in groundwater and drinking water because it causes health problem of arsenicosis after consumption of drinking arsenic-rich water more than 5-10 years. Arsenic contamination of groundwater is an emerging issue in Mekong Basin including Cambodia, Vietnam, and Thailand. In Thailand, information about arsenic contamination in drinking water resources are quite rare due to that arsenic is not the main element in water qualification assay. The objective of this study is to determine groundwater quality and arsenic contamination in rural Mekong Basin, Ubon Ratchathani. Groundwater samples were collected from 20 different sampling points, between August 2009 and February 2010 in Amphoe Khemmarat, Ubon Ratchathani, Thailand. Physical and chemical characteristics of groundwater were determined. It was observed that the groundwater was 27.9-30.3 ~C, pH 5.7-6.9. The conductivity was 707-767 p.S.cm＂. Dissolved oxygen was 2.04-5.12 mg.L-1 and TDS was 352-384 mg.L~. The samples showed soft- to very hardness-water properties. In some area, few parameters like CI, Fe, Mn, and As exceeded the WHO guideline limits. This result represents basic information for quality of groundwater and the arsenic contamination in rural Mekong Basin, Ubon Ratchathani. Thus, it is probably useful for arsenic standard level assignment and public health authorities. Moreover, it also leads to establish research activity in treatment of arsenic-contaminated groundwater for different purposes展开更多
文摘Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co cultivated with Agrobacterium tumefaciens , LBA4404, which contains plasmid vector pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4 D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.
文摘This paper reports the influences of the herbicide butachlor( n butoxymethl chloro 2', 6' diethylacetnilide) on microbial respiration, nitrogen fixation and nitrification, and on the activities of dehydrogenase and hydrogen peroxidase in paddy soil. The results showed that after application of butachlor with concentrations of 5.5 μg/g dried soil, 11.0 μg/g dried soil and 22.0 μg/g dried soil, the application of butachlor enhanced the activity of dehydrogenase at increasing concentrations. The soil dehydrogenase showed the highest activity on the 16th day after application of 22.0 μg/g dried soil of butachlor. The hydrogen peroxidase could be stimulated by butachlor. The soil respiration was depressed within a period from several days to more than 20 days, depending on concentrations of butachlor applied. Both the nitrogen fixation and nitrification were stimulated in the beginning but reduced greatly afterwards in paddy soil.
文摘Arsenic （As） is one of the most important elemental pollutants in groundwater and drinking water because it causes health problem of arsenicosis after consumption of drinking arsenic-rich water more than 5-10 years. Arsenic contamination of groundwater is an emerging issue in Mekong Basin including Cambodia, Vietnam, and Thailand. In Thailand, information about arsenic contamination in drinking water resources are quite rare due to that arsenic is not the main element in water qualification assay. The objective of this study is to determine groundwater quality and arsenic contamination in rural Mekong Basin, Ubon Ratchathani. Groundwater samples were collected from 20 different sampling points, between August 2009 and February 2010 in Amphoe Khemmarat, Ubon Ratchathani, Thailand. Physical and chemical characteristics of groundwater were determined. It was observed that the groundwater was 27.9-30.3 ~C, pH 5.7-6.9. The conductivity was 707-767 p.S.cm＂. Dissolved oxygen was 2.04-5.12 mg.L-1 and TDS was 352-384 mg.L~. The samples showed soft- to very hardness-water properties. In some area, few parameters like CI, Fe, Mn, and As exceeded the WHO guideline limits. This result represents basic information for quality of groundwater and the arsenic contamination in rural Mekong Basin, Ubon Ratchathani. Thus, it is probably useful for arsenic standard level assignment and public health authorities. Moreover, it also leads to establish research activity in treatment of arsenic-contaminated groundwater for different purposes
