Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma

AIM: To construct subtracted cDNA libraries of human vascular endothelial cells (VECs) related to gastrocarcinoma using suppression substractive hybridization (SSH) and to analyze cDNA libraries of gastrocarcinoma and VECs in Cancer Gene Anatomy Project (CGAP) database.METHODS: Human VECs related to gastric adenocarcinoma and corresponding normal tissue were separated by magnetic beads coupled with antibody CD31 (Dynabeads CD31). A few amount of total RNA were synthesized and amplified by SMARTTM PCR cDNA Synthesis Kit. Then, using SSH and T/A cloning techniques, cDNA fragments of differentially expressed genes in human VECs of gastric adenocarcinoma were inserted into JM109 bacteria. One hundred positive bacteria clones were randomly picked and identified by colony PCR method. To analyze cDNA libraries of gastrocarcinoma and VECs in CGAP database, the tools of Library Finder,cDNA xProfiler, Digital GENE Expression Displayer (DGED),and Digital Differential Display (DDD) were used.RESULTS: Forward and reverse subtraction cDNA libraries of human VECs related to gastrocarcinoma were constructed successfully with SSH and T/A cloning techniques. Analysis of CGAP database indicated that no appropriate library of VECs related to carcinoma was constructed.CONCLUSION: Construction of subtraction cDNA libraries of human VECs related to gastrocarcinoma was successful and necessary, which laid a foundation for screening and cloning new and specific genes of VECs related to gastrocardnoma.

Rosiglitazone improves post-infarction left ventricular contractile function in rats

Peroxisome proliferator-activated receptor-γ (PPAR-γ) is a transcription factor belonging to the nuclear receptor superfamily that binds to specific response elements. Rosiglitazone, promotes insulin sensitization and is a synthetic agonist ligand of PPAR-γ. 1 In recent years, a number of studies have shown that triglitazone and piglitazone, which are chemically related to rosiglitazone, can attenuate postinfarction left ventricular remodeling, 2 but there are few studies assessing whether rosiglitazone similarly improves postinfarction left ventricular remodeling and cardiac function. Therefore, the purpose of this study was to determine whether rosiglitazone can improve left ventricular remodeling and cardiac function in a rat model of post-myocardial infarction (MI), and to explore potential underlying mechanisms.