The effect of hMSH2 enzyme-deficiency on the cell growing phenotypes,cell ultrastructure,growth character and cell cycle were observed with electronic microscopy examination,cell counting and flow cytometry.hMSH2-defi...The effect of hMSH2 enzyme-deficiency on the cell growing phenotypes,cell ultrastructure,growth character and cell cycle were observed with electronic microscopy examination,cell counting and flow cytometry.hMSH2-deficient cell strain was constructed by transfecting hMSH2 recombination plasmid of antisense RNA into human embryo lung fibroblasts(HLF).In hMSH2-deficient cells,there were a lot of morphological changes under electronic microscopy,such as irregular shape,a lot of protuberances on the surface of cell,the enlarged nuclei.The average time of double increment of HLF and hMSH2-deficient cells were 1.0d and 0.78d,respectively.This suggested that the cell proliferation of hMSH2-deficient cells was greater than that of HLF.The distribution of HLF and hMSH2-deficient cells in G1,G2 and S phases was different.A large part of hMSH2-deficient cells was blocked in G1 phase.hMSH2-deficient cells increased,but it is still not a typical malignant cells.Thus,this cell strain could be used as biologic material to detect mutagenesis of environmental chemicals.展开更多
文摘The effect of hMSH2 enzyme-deficiency on the cell growing phenotypes,cell ultrastructure,growth character and cell cycle were observed with electronic microscopy examination,cell counting and flow cytometry.hMSH2-deficient cell strain was constructed by transfecting hMSH2 recombination plasmid of antisense RNA into human embryo lung fibroblasts(HLF).In hMSH2-deficient cells,there were a lot of morphological changes under electronic microscopy,such as irregular shape,a lot of protuberances on the surface of cell,the enlarged nuclei.The average time of double increment of HLF and hMSH2-deficient cells were 1.0d and 0.78d,respectively.This suggested that the cell proliferation of hMSH2-deficient cells was greater than that of HLF.The distribution of HLF and hMSH2-deficient cells in G1,G2 and S phases was different.A large part of hMSH2-deficient cells was blocked in G1 phase.hMSH2-deficient cells increased,but it is still not a typical malignant cells.Thus,this cell strain could be used as biologic material to detect mutagenesis of environmental chemicals.
