期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Cloning of human 15ku selenoprotein gene from H9 T cells
1
作者 Ke-JunNan Chun-LiLi +5 位作者 Yong-ChangWei Chen-GuangSui ZhaoJing Hai-XiaQin Li-JunZhao Bo-RongPan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第8期1777-1780,共4页
AIM: To clone human 15ku selenoprotein gene.METHODS: H9 human T cells were cultured in RPMI1640medium supplemented with 100 mL/L fetal calf serum. mRNA was isolated from the cells. cDNA library was constructed by RT-P... AIM: To clone human 15ku selenoprotein gene.METHODS: H9 human T cells were cultured in RPMI1640medium supplemented with 100 mL/L fetal calf serum. mRNA was isolated from the cells. cDNA library was constructed by RT-PCR. The human 15ku selenoprotein gene was obtained by PCR and cloned into T vector and sequenced.RESULTS: A unique cDNA fragment about 1 244 bp was obtained. Sequence analysis identified an open reading frame within the cDNA. The gene had an in-frame TGA, which encoded selenocysteine (Sec), and a 3′-UTR SECIS element,which was required for synthesis of selenoprotein. The predicted protein molecular mass was about 15ku (162residues). The result was identical with human liver 15ku selenoprotein gene published in Genbank.CONCLUSION: Human 15ku selenoprotein gene can be successfully obtained from T cell line. 展开更多
关键词 硒蛋白基因 T细胞 聚合酶链反应 CDNA文库
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部