Tuberculosis (TB) is the most common cause of death in infectious diseases; it is estimated that approximately 2 million people per year die of TB. The present available TB vaccine is a live attenuated strain, Mycoba...Tuberculosis (TB) is the most common cause of death in infectious diseases; it is estimated that approximately 2 million people per year die of TB. The present available TB vaccine is a live attenuated strain, Mycobacterium bovis Bacillus Calmette Guérin (BCG). However, it has been shown that BCG has variable protective efficacy, ranging from 0 to 85% in different clinical experiments. 1 Therefore, a new TB vaccine is urgently needed. Many trials have been done to develop the second-generation TB vaccines in recent years; candidates include avirulent, auxotrophic, subunit, DNA, and recombinant vaccines. 2 The outcomes of these vaccines disappointed investigators. Encouragingly, recombinant BCG, which overexpressed the mycobacterial antigen Ag85B, produced an excellent protective response, 3,4 suggesting that a vaccine based on recombinant BCG technique was a potential approach against TB.展开更多
IDDM results from pancreatic beta cell destruction by islet-reactive T cells,a process that involves beta cell apoptosis.Fas-FasL pathway plays a major role in pancreatic β cell death.Fas-associated death domain prot...IDDM results from pancreatic beta cell destruction by islet-reactive T cells,a process that involves beta cell apoptosis.Fas-FasL pathway plays a major role in pancreatic β cell death.Fas-associated death domain protein (FADD),the component of the tumor necrosis factor receptor type 1(TNFR1) and Fas signaling complexes,is involved in TNFR1-and Fas-induced apoptosis.Inhibiting the function of FADD will lead to blocking downstream apoptosis signal,which protects pancreatic β cells from destruction by Fas-FasL pathway.In this study we constructed eukaryotic expressing vector of fusional protein FADDdel-GFP named pFADDdel-GFP. After pFADDdel-GFP was transfected into NIT,the expression of FADDdel-GFP in NIT was detected by fluorescence microscopy and the resistance of NIT transfected with pFADDdel-GFP to cytotoxicity mediated by special T cells was detected by FACS and MTT.The results showed that NIT modified by pFADDdel-GFP obviously resisted cytotoxicity mediated by special T cells.Therefore,it may be useful in the prevention or treatment of IDDM by intervening Fas-FasL pathway.Cellular & Molecular Immunology.2004;1(5):383-386.展开更多
文摘Tuberculosis (TB) is the most common cause of death in infectious diseases; it is estimated that approximately 2 million people per year die of TB. The present available TB vaccine is a live attenuated strain, Mycobacterium bovis Bacillus Calmette Guérin (BCG). However, it has been shown that BCG has variable protective efficacy, ranging from 0 to 85% in different clinical experiments. 1 Therefore, a new TB vaccine is urgently needed. Many trials have been done to develop the second-generation TB vaccines in recent years; candidates include avirulent, auxotrophic, subunit, DNA, and recombinant vaccines. 2 The outcomes of these vaccines disappointed investigators. Encouragingly, recombinant BCG, which overexpressed the mycobacterial antigen Ag85B, produced an excellent protective response, 3,4 suggesting that a vaccine based on recombinant BCG technique was a potential approach against TB.
基金This study was supported by National Key Basic Research Program of China(No.CB5 10008)National Natural Science foundation(No.30300166).
文摘IDDM results from pancreatic beta cell destruction by islet-reactive T cells,a process that involves beta cell apoptosis.Fas-FasL pathway plays a major role in pancreatic β cell death.Fas-associated death domain protein (FADD),the component of the tumor necrosis factor receptor type 1(TNFR1) and Fas signaling complexes,is involved in TNFR1-and Fas-induced apoptosis.Inhibiting the function of FADD will lead to blocking downstream apoptosis signal,which protects pancreatic β cells from destruction by Fas-FasL pathway.In this study we constructed eukaryotic expressing vector of fusional protein FADDdel-GFP named pFADDdel-GFP. After pFADDdel-GFP was transfected into NIT,the expression of FADDdel-GFP in NIT was detected by fluorescence microscopy and the resistance of NIT transfected with pFADDdel-GFP to cytotoxicity mediated by special T cells was detected by FACS and MTT.The results showed that NIT modified by pFADDdel-GFP obviously resisted cytotoxicity mediated by special T cells.Therefore,it may be useful in the prevention or treatment of IDDM by intervening Fas-FasL pathway.Cellular & Molecular Immunology.2004;1(5):383-386.
