11C-labeled C1-C10 partial structure of kulokekahilide-2 (1) was successfully synthesized based on Pd0-mediated rapid C-[11C]methylation using [11C]methyl iodide and pinacol alkenylboronate. The preparation of organob...11C-labeled C1-C10 partial structure of kulokekahilide-2 (1) was successfully synthesized based on Pd0-mediated rapid C-[11C]methylation using [11C]methyl iodide and pinacol alkenylboronate. The preparation of organoboron intermediate via olefin cross-metathesis is also a crucial procedure for the synthesis of 11C-labeling C1-C10 dihy-droxy acid moiety of 1.展开更多
Obesity is a major worldwide health problem that is related to most chronic diseases,including male infertility.Owing to its wide impact on health,mechanisms underlying obesity-related infertility remain unknown.In th...Obesity is a major worldwide health problem that is related to most chronic diseases,including male infertility.Owing to its wide impact on health,mechanisms underlying obesity-related infertility remain unknown.In this study,we report that mice fed a high-fat diet(HFD)for over 2 months showed reduced fertility rates and increased germ cell apoptosis,seminiferous tubule degeneration,and decreased intratesticular estradiol(E2)and E2-to-testosterone ratio.Interestingly,we also detected a decrease in testicular fatty acid levels,behenic acid(C22:0),and docosahexaenoic acid(DHA,22:6n-3),which may be related to the production of dysfunctional spermatozoa.Overall,we did not detect any changes in the frequency of seminiferous tubule stages,sperm count,or rate of in vitro capacitation.However,there was an increase in spontaneous and progesterone-induced acrosomal exocytosis(acrosome reaction)in spermatozoa from HFD-fed mice.These data suggest that a decrease in E2 and fatty acid levels influences spermatogenesis and some steps of acrosome biogenesis that will have consequences for fertilization.Thus,our results add new evidence about the adverse effect of obesity in male reproduction and suggest that the acrosomal reaction can also be affected under this condition.展开更多
Pyrene solution and distilled water were flowed through a microreactor at a predetermined flow rate to generate pyrene crystals. Pyrene nanocrystals were crystallized by a Continuous Flow Microreactor. The particle si...Pyrene solution and distilled water were flowed through a microreactor at a predetermined flow rate to generate pyrene crystals. Pyrene nanocrystals were crystallized by a Continuous Flow Microreactor. The particle size and luminescence properties of pyrene nanocrystals produced were evaluated. The crystal mean size between 60 nm and 400 nm could be controlled by the operating conditions. The crystal mean size decreased with increasing flow rate and solution concentration. In addition, the crystal morphology also changed. In the case of slow flow conditions, a needle-like crystal morphology was obtained. The crystal morphology became spherical on increasing the flow rate. Pyrene crystals with size about 400 nm exhibited luminescence at about 470 nm. As the crystal size decreased, the intensity of the luminescence also declined. The luminescence wavelength was in the range of about 370 to 400 nm.展开更多
文摘11C-labeled C1-C10 partial structure of kulokekahilide-2 (1) was successfully synthesized based on Pd0-mediated rapid C-[11C]methylation using [11C]methyl iodide and pinacol alkenylboronate. The preparation of organoboron intermediate via olefin cross-metathesis is also a crucial procedure for the synthesis of 11C-labeling C1-C10 dihy-droxy acid moiety of 1.
文摘Obesity is a major worldwide health problem that is related to most chronic diseases,including male infertility.Owing to its wide impact on health,mechanisms underlying obesity-related infertility remain unknown.In this study,we report that mice fed a high-fat diet(HFD)for over 2 months showed reduced fertility rates and increased germ cell apoptosis,seminiferous tubule degeneration,and decreased intratesticular estradiol(E2)and E2-to-testosterone ratio.Interestingly,we also detected a decrease in testicular fatty acid levels,behenic acid(C22:0),and docosahexaenoic acid(DHA,22:6n-3),which may be related to the production of dysfunctional spermatozoa.Overall,we did not detect any changes in the frequency of seminiferous tubule stages,sperm count,or rate of in vitro capacitation.However,there was an increase in spontaneous and progesterone-induced acrosomal exocytosis(acrosome reaction)in spermatozoa from HFD-fed mice.These data suggest that a decrease in E2 and fatty acid levels influences spermatogenesis and some steps of acrosome biogenesis that will have consequences for fertilization.Thus,our results add new evidence about the adverse effect of obesity in male reproduction and suggest that the acrosomal reaction can also be affected under this condition.
文摘Pyrene solution and distilled water were flowed through a microreactor at a predetermined flow rate to generate pyrene crystals. Pyrene nanocrystals were crystallized by a Continuous Flow Microreactor. The particle size and luminescence properties of pyrene nanocrystals produced were evaluated. The crystal mean size between 60 nm and 400 nm could be controlled by the operating conditions. The crystal mean size decreased with increasing flow rate and solution concentration. In addition, the crystal morphology also changed. In the case of slow flow conditions, a needle-like crystal morphology was obtained. The crystal morphology became spherical on increasing the flow rate. Pyrene crystals with size about 400 nm exhibited luminescence at about 470 nm. As the crystal size decreased, the intensity of the luminescence also declined. The luminescence wavelength was in the range of about 370 to 400 nm.
