Objectives To study the interaction of beta-2-glycoprotein I (β2GP I ) with the membrane of hepatocytes and determine whether β2GP I participates in HBV infection.Methods Ligand blotting, fluorescence microscopy, an...Objectives To study the interaction of beta-2-glycoprotein I (β2GP I ) with the membrane of hepatocytes and determine whether β2GP I participates in HBV infection.Methods Ligand blotting, fluorescence microscopy, and fluorescence activated cell sorter (FACS) analysis were used to detect the specific interaction of β2GP I with the hepatoma cell line smmc7721, the gastric carcinoma cell line SGC7901, and the lymphoma cell line HL-60.Results A specific 40 kDa β2GP I band was observed by ligand blotting in the case of smmc7721 cells. No such band was observed in SGC7901 or HL-60 cells. Fluorescence microscopy also revealed specific binding of FITC-β2GP I to smmc7721 cells, but neither to SGC7901 nor HL-60 cells. FACS analysis demonstrated that the binding rate of FITC-β2GP I to smmc7721 cells was significantly higher than these in SGC7901 and HL-60 cells (P<0. 01). The binding rate to smmc7721 cells did not increase with increasing amounts of FITC-β2GP I .Conclusions There is a specificβ2GP I -binding protein on the membrane of hepatoma cells in cell line smmc7721 which as the β2 GP I receptor may participate in HBV infection of hepatocytes.展开更多
基金This study was supported by the National Natural Science Foundation of China (No.30070338).
文摘Objectives To study the interaction of beta-2-glycoprotein I (β2GP I ) with the membrane of hepatocytes and determine whether β2GP I participates in HBV infection.Methods Ligand blotting, fluorescence microscopy, and fluorescence activated cell sorter (FACS) analysis were used to detect the specific interaction of β2GP I with the hepatoma cell line smmc7721, the gastric carcinoma cell line SGC7901, and the lymphoma cell line HL-60.Results A specific 40 kDa β2GP I band was observed by ligand blotting in the case of smmc7721 cells. No such band was observed in SGC7901 or HL-60 cells. Fluorescence microscopy also revealed specific binding of FITC-β2GP I to smmc7721 cells, but neither to SGC7901 nor HL-60 cells. FACS analysis demonstrated that the binding rate of FITC-β2GP I to smmc7721 cells was significantly higher than these in SGC7901 and HL-60 cells (P<0. 01). The binding rate to smmc7721 cells did not increase with increasing amounts of FITC-β2GP I .Conclusions There is a specificβ2GP I -binding protein on the membrane of hepatoma cells in cell line smmc7721 which as the β2 GP I receptor may participate in HBV infection of hepatocytes.
