Epidermal growth factor receptor (EGFR) has been shown to play an important role in the malignant progression of diffuse astrocytomas. Numerous studies have investigated the clinical significance of overexpression and...Epidermal growth factor receptor (EGFR) has been shown to play an important role in the malignant progression of diffuse astrocytomas. Numerous studies have investigated the clinical significance of overexpression and amplification of the EGFR gene in these tumours with ambiguous results. The aim of this study was therefore to review the literature to get the current prognostic and diagnostic status. The PubMed database was used to search for papers in English in the time period 1987-2010 using the following keywords: astrocytoma, glioma, c-erbB1, EGFR, glioblastoma, HER1 and prognosis. Other inclusion criteria were: 1) studies with more than 50 patients;2) patient age over 18 years;3) tumour grading according to the latest World Health Organization (WHO) classification (2007) and 4) EGFR overexpression assessed by immunohistochemistry. A total of 47 publications were included and several reported positive correlations between both EGFR gene amplification and overexpression with histological malignancy grade and survival. The results are, however, encumbered with elements of uncertainty involving for instance methodological diversities and small number of patients. Larger studies with standardized procedures are necessary to fully clarify the clinical role of EGFR in human astrocytomas.展开更多
Acute myeloid leukemia （AML） M4Eo type is a hematological malignancy with abnormal eosinophilia, which is often accompanied by inv（16）. The Ets variant gene 6 （ETV6）, mapped to 12p13, is an ETS family transcript...Acute myeloid leukemia （AML） M4Eo type is a hematological malignancy with abnormal eosinophilia, which is often accompanied by inv（16）. The Ets variant gene 6 （ETV6）, mapped to 12p13, is an ETS family transcription factor that is essential for hematopoietic processes,l The ETV6 gene-involved chromosomal translocations have been found in many hematological malignancies characterized by fusing to a number of different partner genes; mainly coding for tyrosine kinases or transcription factors which are important for the initiation, progress and prognosis of disease.2 In particular, the ETV6 gene has been reported to be fused to ABL in acute lymphocytic leukemias （ALL）,3 and chronic myeloid leukemia （CML）.4 However, there have been few domestic reports of ETV6 fusion genes, especially in cases of acute leukemia. We investigated 3 cases of AML-M4Eo patients using Split-signal Fluorescence in situ hybridization （FISH） and found one case with a translocation between 12p13 and lq25 co-occurring with an inv（16）. The ETV6/ARG （ABL-related gene） fusion transcript was confirmed by reverse transcriptase-polymerase （RT-PCR）. This is the report of ARG chain reaction involvement in a translocation in a human malignancy.展开更多
文摘Epidermal growth factor receptor (EGFR) has been shown to play an important role in the malignant progression of diffuse astrocytomas. Numerous studies have investigated the clinical significance of overexpression and amplification of the EGFR gene in these tumours with ambiguous results. The aim of this study was therefore to review the literature to get the current prognostic and diagnostic status. The PubMed database was used to search for papers in English in the time period 1987-2010 using the following keywords: astrocytoma, glioma, c-erbB1, EGFR, glioblastoma, HER1 and prognosis. Other inclusion criteria were: 1) studies with more than 50 patients;2) patient age over 18 years;3) tumour grading according to the latest World Health Organization (WHO) classification (2007) and 4) EGFR overexpression assessed by immunohistochemistry. A total of 47 publications were included and several reported positive correlations between both EGFR gene amplification and overexpression with histological malignancy grade and survival. The results are, however, encumbered with elements of uncertainty involving for instance methodological diversities and small number of patients. Larger studies with standardized procedures are necessary to fully clarify the clinical role of EGFR in human astrocytomas.
文摘Acute myeloid leukemia （AML） M4Eo type is a hematological malignancy with abnormal eosinophilia, which is often accompanied by inv（16）. The Ets variant gene 6 （ETV6）, mapped to 12p13, is an ETS family transcription factor that is essential for hematopoietic processes,l The ETV6 gene-involved chromosomal translocations have been found in many hematological malignancies characterized by fusing to a number of different partner genes; mainly coding for tyrosine kinases or transcription factors which are important for the initiation, progress and prognosis of disease.2 In particular, the ETV6 gene has been reported to be fused to ABL in acute lymphocytic leukemias （ALL）,3 and chronic myeloid leukemia （CML）.4 However, there have been few domestic reports of ETV6 fusion genes, especially in cases of acute leukemia. We investigated 3 cases of AML-M4Eo patients using Split-signal Fluorescence in situ hybridization （FISH） and found one case with a translocation between 12p13 and lq25 co-occurring with an inv（16）. The ETV6/ARG （ABL-related gene） fusion transcript was confirmed by reverse transcriptase-polymerase （RT-PCR）. This is the report of ARG chain reaction involvement in a translocation in a human malignancy.
