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High glucose impairs glucose metabolism and inhibits osteoblast differentiation via primary cilia/Hedgehog pathway in mouse embryonic osteoblast precursor cells
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作者 LIU Jie 《China Medical Abstracts(Internal Medicine)》 2022年第1期29-29,共1页
Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with g... Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with glucose a-MEM at concentrations of 5.6 mmol/L(Con group)and 25.0 mmol/L(HG group).Cell proliferation was detected by CCK-8 and Ki67 immunofluorescence. 展开更多
关键词 METABOLISM KI67 HEDGEHOG
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