Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with g...Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with glucose a-MEM at concentrations of 5.6 mmol/L(Con group)and 25.0 mmol/L(HG group).Cell proliferation was detected by CCK-8 and Ki67 immunofluorescence.展开更多
文摘Objective To study the effects of high glucose on the proliferation,osteoblast differentiation and glucose metabolism of mouse embryonic osteoblast precursor cells(MC3T3-E1).Methods MC3T3-E1 cells were cultured with glucose a-MEM at concentrations of 5.6 mmol/L(Con group)and 25.0 mmol/L(HG group).Cell proliferation was detected by CCK-8 and Ki67 immunofluorescence.