Biological variation in 32 clinical laboratory routine tests

Objective To set quality goals of conventional biochemical tests through the research of biological variation of the 32 routine items in Chinese population to provide the basis for Chinese clinical and laboratory standards. Methods According to the experimental designs and computing

Selection and evaluation of clinical diagnostic cut point of glycated albumin

Objective To investigate the relationship between GA and HbA1c,to identify the optimal GA cut-off values used to diagnose preDM and DM,and to verify the diagnostic significance of blood glucose biomarkers.Methods A total of 20 491 samples from Chinese people were involved in the correlation analysis.The participants were expected to complete the test of FPG,HbA1cand GA.

The role of a novel protein ligand hMSH 2 in Vγ9δ2 T cell-mediated anti-cervix cancer immunity

Objective To explore the role of hMSH2,a novel endogenous tumor-associated protein ligand recognized by Vγ9δ2 T cells,in innate anti-cervix cancer immunity.Methods hMSH2 that expressed on the surface of cervical cancer cell line HeLa cells was blocked by specific antibody.Then the differences in their effects on Vγ9δ2T cells before and after antibody blockage were evaluated by cytotoxicity of Vγ9δ2 T cells and cytokines secretion.

Loss of p120 catenin aggravates alveolar edema of ventilation induced lung injury

Background p120 catenin （p120ctn） is an adheren junction protein that regulates barrier function, but its role has not been explored in alveolar edema induced by ventilation. We measured stretch-induced cell gap formation in MLE 12 cells due to the loss of p120. We hypothesized that alveolar permeability was increased by high tung inflation associated with alveolar epithelia cell tight junctions being destroyed, which resulted from the loss of p120. Methods Cultured MLE12 cells were subjected to being stretched or un-stretched （control） and some cells were pretreated with pp2 （c-src inhibitor）. After the end of stretching for 0, 1, 2, and 4 hours, the cells were lysed, and p120 expression and c-src activation was determined by Western blotting analysis. In vivo, SD rats were taken to different tidal volumes （Vt 7 ml/kg or 40 ml/kg, PEEP=0, respiratory rate 30-40 betas/min） for 0, 1, 2, and 4 hour and some were pretreated with pp2, and alveolar edema was calculated. Rerults It was found that p120 expression was reduced and c-src activation increased in a time-dependent and strain- dependent manner due to cyclic-stretch of the alveolar epithelial cells. These changes could be reversed by inhibition of c-src. We obtained similar changes in rats when they were subjected to large tidal volumes and the alveolar edema increased more than in rats in the low Vt group. Pretreated the rats with inhibition of c-src had less pulmonary edema induced by the high tidal volume ventilation. Conclusions Cyclic stretch MLE 12 cells induced the loss of p120 and may be the same reason by high tidal volume ventilation in rats can aggravate alveolar edema. Maintenance of p120 expression may be a novel therapeutic strategy for the prevention and treatment of ventilation induced lung injury （VILI）.

Antimicrobial susceptibility of communityacquired respiratory tract pathogens isolated from class B hospitals in China during 2013 and 2014

Objective To investigate the drug-resistance rate of community-acquired respiratory tract pathogens isolated from class B hospitals in China during 2013 and 2014.Methods A total of 860 strains(S.pneumoniae 299,K.pneumoniae 221,H.influenzae 185,S.aureus 116。

IGF-1 inhibited the apoptosis of gastric smooth muscle cells induced by high glucose through AMPK-Akt-mTOR pathway

Objective To investigate the effect of insulin-like growth factor 1(IGF-1)on rat gastric smooth muscle cell apoptosis and key proteins of AMPK-Akt-mTOR pathway and to explore the related mechanisms.Methods Rat gastric smooth muscle cells cultured in vitro were divided into two groups:high glucose group(HG group)and high glucose and IGF-1 group(HG+IGF-1 group).

Study on vitamin D levels in serum and intestinal mucosa of 37 adult patients with celiac disease

Objective To analyze the expression of 25-hydroxyvitamin D[25(OH)D]in serum and vitamin D receptor(VDR)in descending duodenum of adult patients with celiac disease and the correlation between 25(OH)D,VDR and the clinical characteristics and indexes of celiac disease.Methods From September 1.

Association between pulse pressure and new-onset diabetes in hypertensive patients

Objective To determine the association between pulse pressure and the risk of new-onset diabetes in hypertensive patients.MethodsIn this prospective cohort study,hypertensive patients from the Kailuan Study,who were diagnosed in 2006-2007 check-up,were screened for enrollment.

Association between genetic polymorphism in haptoglobin and kidney injury in patients with type 2 diabetes mellitus

Objective To explore the distribution characteristics of haptoglobin(Hp)genotype and allele frequency in patient with type 2 diabetes mellitus(T2DM)in Han population,and the association between genetic polymorphism in haptoglobin and kidney injury indexes.

MiR-181a and miR-181b influence proliferation,migration, invasion and angiogenesis via targeting FUT1 in colorectal cancer

Objective To investigate the correlation of miR-181a and miR-181b with fucosyltransferase FUT1, the functional mechanism was elucidated in a colorectal cancer (CRC). Methods We collected 32 pairs of tissue samples, 18 males and 14 females in the first affiliated hospital of Dalian Medicinal University.

The differential diagnostic value of pleural effusion heparin-binding protein in parapneumonic effusion

Objective The aim of this study was to evaluate the value of pleural effusion heparin-binding protein( HBP) in differential diagnosis of parapneumonic effusion.Methods Case-control study. The pleural effusion of 189 patients with pleural effusion admitted to Quzhou People's Hospital from February to July 2018.