Objective To evaluate the significance and effect of nasal aesthetic sub-unit principle.Methods Based on nasal aesthetic sub-unit,27 patients were divided into 5 groups according to their nasal defects.They were nasal...Objective To evaluate the significance and effect of nasal aesthetic sub-unit principle.Methods Based on nasal aesthetic sub-unit,27 patients were divided into 5 groups according to their nasal defects.They were nasal dorsum,tip,alar lobule,soft triangle and two or two above nasal subunit defects groups.Local restore were applied in defects which were less than 50 percent of one nasal subunit.展开更多
To investigate the surgical methods of treating severely injured vagina.Methods A retrospective study was carried out on the clinical materials and results of 7 patients suffered from severe vaginal injury.Results Dif...To investigate the surgical methods of treating severely injured vagina.Methods A retrospective study was carried out on the clinical materials and results of 7 patients suffered from severe vaginal injury.Results Different surgical approaches such as trans-suprapubic,transperineal or both approaches were applied according to the position of the atresia,meanwhile,different surgical methods such as skin graft,free flap transplantation,vaginal mucous flap advancement,direct anastomosis of the two ends,“Z” plasty was used to repair the vagina according to the different defect of the vagina,except for 1 cases complicated with vaginorecturs fistula,all the other cases were achieved satisfactory results.Conclusion The accurate judgment of the severity of the vaginal injury and the understanding of the changed anatomy around the injured vagina was the key points to the success of the surgical treatment of traumatic vaginal atresia.4 refs,3 figs,1 tab.展开更多
Objective To investigate the methods of isolating and identifying human adipose derived EPCs.Methods The cells obtained from human lipoaspirates were plated on culture dishes coated with human fibronectin and were cul...Objective To investigate the methods of isolating and identifying human adipose derived EPCs.Methods The cells obtained from human lipoaspirates were plated on culture dishes coated with human fibronectin and were cultured in DMEM containing 2% FBS.Cells of passage 2 cultured in EGM-2 （2% FBS） served as the induced cells （experimental group）,with cells cultured in DMEM （2% FBS） as the noninduced cells （control group）.展开更多
Objective To repair segmental mandibular defects with autologous bone marrow stromal cells （BMSCs） engineered bone.Methods Isolated BMSCs were expanded in vitro and osteogenic induced.In 12canines,a 3cm segmental ma...Objective To repair segmental mandibular defects with autologous bone marrow stromal cells （BMSCs） engineered bone.Methods Isolated BMSCs were expanded in vitro and osteogenic induced.In 12canines,a 3cm segmental mandibular defect at right mandible was created.6 canine＇s defects were repaired with cell-scaffold constructs made from induced BMSCs and coral;others were repaired with coral as control.展开更多
Objective To evaluate the effect of insulin-like growth factor 1 for the bone induction and the regulation for the fusion of the sagittal cranial sutures. Methods The cells, derived from cranial sutures in the newborn...Objective To evaluate the effect of insulin-like growth factor 1 for the bone induction and the regulation for the fusion of the sagittal cranial sutures. Methods The cells, derived from cranial sutures in the newborn SD rats and the sagittal suture from the mice, were cultured with a serum-free medium and treated with and without insulin-like growth factor 1. The osteoblast phetotypes (osteocalcin, alkaline, osteoponcin and type-1 collagen) were measured with the RT -PCR and ELISA, and the explanted sagittal sutures were then evaluated under light microscopy. Results The cells, treated with the insulin-like growth factor 1, significantly produced more osteocalcin, alkaline, osteoponcin and type-1 collagen than those without insulin-like growth factor 1. The fusion of the sagittal suture explants will delay till to 30 days when it was not treated with IGF1. However, in the group with IGF1 the fusion was observed to start in 8 days, and a small amount of the sagittal suture fusion was found at the 20th展开更多
文摘Objective To evaluate the significance and effect of nasal aesthetic sub-unit principle.Methods Based on nasal aesthetic sub-unit,27 patients were divided into 5 groups according to their nasal defects.They were nasal dorsum,tip,alar lobule,soft triangle and two or two above nasal subunit defects groups.Local restore were applied in defects which were less than 50 percent of one nasal subunit.
文摘To investigate the surgical methods of treating severely injured vagina.Methods A retrospective study was carried out on the clinical materials and results of 7 patients suffered from severe vaginal injury.Results Different surgical approaches such as trans-suprapubic,transperineal or both approaches were applied according to the position of the atresia,meanwhile,different surgical methods such as skin graft,free flap transplantation,vaginal mucous flap advancement,direct anastomosis of the two ends,“Z” plasty was used to repair the vagina according to the different defect of the vagina,except for 1 cases complicated with vaginorecturs fistula,all the other cases were achieved satisfactory results.Conclusion The accurate judgment of the severity of the vaginal injury and the understanding of the changed anatomy around the injured vagina was the key points to the success of the surgical treatment of traumatic vaginal atresia.4 refs,3 figs,1 tab.
文摘Objective To investigate the methods of isolating and identifying human adipose derived EPCs.Methods The cells obtained from human lipoaspirates were plated on culture dishes coated with human fibronectin and were cultured in DMEM containing 2% FBS.Cells of passage 2 cultured in EGM-2 （2% FBS） served as the induced cells （experimental group）,with cells cultured in DMEM （2% FBS） as the noninduced cells （control group）.
文摘Objective To repair segmental mandibular defects with autologous bone marrow stromal cells （BMSCs） engineered bone.Methods Isolated BMSCs were expanded in vitro and osteogenic induced.In 12canines,a 3cm segmental mandibular defect at right mandible was created.6 canine＇s defects were repaired with cell-scaffold constructs made from induced BMSCs and coral;others were repaired with coral as control.
文摘Objective To evaluate the effect of insulin-like growth factor 1 for the bone induction and the regulation for the fusion of the sagittal cranial sutures. Methods The cells, derived from cranial sutures in the newborn SD rats and the sagittal suture from the mice, were cultured with a serum-free medium and treated with and without insulin-like growth factor 1. The osteoblast phetotypes (osteocalcin, alkaline, osteoponcin and type-1 collagen) were measured with the RT -PCR and ELISA, and the explanted sagittal sutures were then evaluated under light microscopy. Results The cells, treated with the insulin-like growth factor 1, significantly produced more osteocalcin, alkaline, osteoponcin and type-1 collagen than those without insulin-like growth factor 1. The fusion of the sagittal suture explants will delay till to 30 days when it was not treated with IGF1. However, in the group with IGF1 the fusion was observed to start in 8 days, and a small amount of the sagittal suture fusion was found at the 20th
