The application of loop-mediated isothermal amplification for detection of common pathogenic bacteria in lower respiratory tract infections

Objective To investigate the spectrum of common pathogenic bacteria of low respiratory tract infection by loop-mediated isothermal amplification(LAMP)of nucleic acid test and to prove the clinical significance of this method.Methods A total of 289 qualified sputum samples from patients with lower respiratory tract infections in Fujian Province were detected by LAMP technique,and then the distribution of pathogenic bacteria was analyzed.The positive cases(the patients whose

The change and significance of vancomycin minimal inhibitory concentration against methicillin-resistant Staphylococcus aureus isolates from inpatients with lower respiratory tract infection

Objective To investigate the change and significance of vancomycin minimal inhibitory concentration(MIC) against methicillin-resistant Staphylococcus aureus(MRSA)isolates.Methods We analyzed the data

Intervention with flexible bronchoscopy in patiens with respiratory failure caused by tracheal stenosis

Objective To investigate the efficiency and safety of intervention with flexible bronchoscope under general anesthesia by using laryngeal mask in patients with severe tracheal stenosis induced respirtory failure.Methods A total of 16 in-patients with respiratory failure caused

Diagnostic value of computed tomographyguided percutaneous needle biopsy versus radial probe endobronchial ultrasound-guided transbronchial lung biopsy in peripheral pulmonary lesions

Objective To evaluate the value of computed tomography-guided percutaneous needle biopsy(CT-PNB)and radial probe endobronchial ultrasound-guided transbronchial lung biopsy(EBUS-TBLB)in the diagnosis of peripheral pulmonary lesions(PPLs).Methods The clinical data of 213 patients who were diagnosed as

Airway remodeling assessed by high-resolution computed tomography in patients with asthma:relationship to biological markers in induced sputum

Objective To explore the significance of assessing asthma control by high-resolution computed tomography(HRCT) and biological markers in induced sputum.Methods Forty-eight patients with asthma(asthma group) and 10 healthy subjects(control group) were retrospectively analyzed.

Relationship between expression of HDAC2,IL-8,TNF-α in lung adenocarcinoma tissues and smoking

Objective To investigate the relationship between the expression of histone acetylation enzyme 2(HDAC2),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)in lung adenocarcinoma tissues and smoking.Methods A total of 73 cases of lung adenocarcinoma confirmed by pathological examination after surgical

Incidence of pulmonary embolism in patients with acute exacerbations of chronic obstructive pulmonary disease:a meta-analysis

Objective To evaluate the incidence of pulmonary embolism in patients with acute exacerbations of chronic obstructive pulmonary disease (COPD) .Methods Comprehensive searches as of June 2012 were performed in PubMed (1966—) ,Embase (1974—) 。

The clinical characteristics analysis of Escherichia coli bloodstream infection

Objective To explore the clinical features of Escherichia coli bloodstream infection.Methods The clinical data of underlying diseases,antimicrobial susceptibility,temperature at blood sampling,results of routine blood tests,venous catheterization,therapy and prognosis of Escherichia coli bloodstream infection in the First Affilia-

Effect of Bufei granules on the serum IL-33/s ST2 axis and related inflammatory cytokines in mild-to-moderate chronic obstructive pulmonary disease stable phase patients: a multi-center,double-blinded,randomized controlled trial

Objective To observe the effect of Bufei Granules on the mild-to-moderate chronic obstructive pulmonary disease(COPD)stable phase patients,and to explore its mechanism from the perspective of regulating the serum levels of interleukin-33(IL-33)/soluble ST2(s ST2)axis and related inflammatory cytokines.Methods A multicenter double-blinded randomized controlled trial was performed.Totally 271 mild-to-moderate COPD stable phase patients from 4 referral hospitals were enrolled and assigned to two groups by stratified block randomization,136 in the treatment group(TG)and 135 in the control group(CG).All the patients received disease education,self-management and integrative care.Additionally,the patients in the TG were treated with Bufei Granules,while the patients in the CG were treated with Bufei Granules placebo,10 g per package,2 packages each time,2 times per day.The therapeutic course for all was 12 weeks.The scores of COPD assessment test questionnaire(CATQ),St.George's Respiratory Questionnaire(SGRQ)and traditional Chinese medicine clinical symptoms(TCMCS)were recorded.The pulmonary function tests including forced expiratory volume in one second(FEV1),forced vital capacity(FVC),FEV1/predicted value(FEV1%)and FEV1/FVC,and the serum levels of IL-33,s ST2,IL-4 and IL-10 of the patients were measured.Results Compared with those before treatment,the scores of CATQ,TCMCS,SGRQ(including the scores of respiratory symptoms,activity limitation,disease affect and total score),and the serum level of s ST2 in the TG decreased obviously after treatment(P<0.01),however the serum levels of IL-33,IL-4 and IL-10 increased significantly(P<0.01),meanwhile were superior to those in the CG(P<0.01).Compared between the two groups,the indexes of FEV1,FVC,FEV1% and FEV1/FVC of the TG were higher than those in the CG after treatment(P<0.05).The total effective rate of TCMCS in the TG was 75.74%(103/136),and was superior to 9.63% in the CG(13/135,X^2=120.929,P<0.01).Conclusion Bufei Granules could effectively treat the mild-to-moderate COPD stable phase patients by ameliorating the clinical symptoms,protecting the pulmonary function from deterioration and improving the life quality.Its effect mechanism might be related to the enhancment of anti-inflammation ability by increasing the serum levels of IL-33,IL-4 and IL-10,and decreasing the serum level of s ST2.

Therapeutic effects and the underlying mechanism of umbilical cord-derived mesenchymal stem cells for bleomycin induced lung injury in rats

Objective To study the efficacy of umbilical cordderived mesenchymal stem cells(UC-MSCs)for bleomycin-induced pulmonary fibrosis in mice.Methods UCMSCs were isolated from the umbilical cord after parental consent.One hundred C57BL/6 mice were randomly divided into 4 groups(12 of these for preliminary experiment).Mice in

A cross-section study of severe asthma in eight provinces of China

Objective To analyze the prevalence of severe asthmain China.Methods The epidemiological data was collected from 2 034 asthmatic patients who were diagnosed in the last epidemiological survey from 2009 to 2010 in 8provinces.Results According to the questionnaire survey,among the 2 034 patients,the previously

The change and its clinical significance of serum cystatin C in elderly patients with obstructive sleep apnea hypopnea syndrome

Objective To investigate the change of serum cystatin C (CC) level and its clinical significance in elderly patients with obstructive sleep apnea hypopnea syndrome (OSAHS) ,and to evaluate the diagnostic value of serum CC for renal impairment in elderly OSAHS patients.Methods Elderly snoring subjects (age≥60 years) un-

The distribution characteristics of etiology of chronic cough in Lanzhou

Objective To explore the distribution characteristics of etiology and clinical feature of chronic cough in Lanzhou.Methods Based on the guidelines of the diag-nostic process of chronic cough in China,data of medical history and physical examinations were collected,and chest X-ray,pulmonary function plus airway hyperresponsiveness,induced sputum eosinophils,sinus

Long-term follow-up study of moderate chronic obstructive pulmonary disease in elderly patients

Objective To investigate the prognosis of moderate chronic obstructive pulmonary disease (COPD) in the elderly patients, and to evaluate the factors for long term survival. Methods From May 1993,a prospective cohort study was carried out,in which 191 elderly patients with moderate COPD were recruited in Shougang communities, Shijingshan District, Beijing. The general

Effects of PM2 5 exposure on Klebsiella pneumoniae clearance in the lungs of rats

Objective To investigate the effects of PM2.5 exposure on susceptibility to Klebsiella infection and bacterial clearance,and to discuss its possible mechanisms.Methods Eighty-six healthy male SD rats were randomly divided into 4 groups:a control group,a Klebsiella pneumoniae infection group(infection group),a PM2.5 group and a PM2.

Effects and significance of methacholine bronchial provocation tests and salbutamol bronchial dilation test on measurements of fractional exhaled nitric oxide in patients with asthma

Objective To study the effects and significance of methacholine(Mch)bronchial provocation tests and salbutamol bronchial dilation test on measurements of fractional exhaled nitric oxide(Fe NO)in patients with asthma.Methods This was a prospective study conducted between November 2014 and August 2015.A total

The clinical features of invasive pulmonary aspergillosis in 53 chronic obstructive pulmonary disease patients

Objective To study the clinical features of invasive pulmonary aspergillosis (IPA) with chronic obstructive pulmonary diseases(COPD),so as to provide evidence for early diagnosis and treatment. Methods A retrospective analysis was made upon clinical date,diagnosis, treatment and

Evaluation of carotid artery elasticity in patients with obstructive sleep apnea syndrome using quantitative arterial stiffness technique

Objective To explore the changes and clinical value of carotid elasticity index in patients with obstructive sleep apnea syndrome (OSAS) by quantitative arterial stiffness(OAS) technique. Methods Seventy-two OSAS patients were divided into 2 groups according to whether there was coexisting

A study on the relevance of airway inflammatory indices in induced sputum and airway hyperresponsiveness in asthmatics

Objective To analyze the correlations between NO3-/NO2-,eosinophil counts in induced sputum and airway hyperresponsiveness (AHR) and therefore to explore the clinical significance of these parameters in severity assessment and medication adjustment in patients with mild to moderate asthma. Methods From February 2003 to June 2004,35 outpatients with mild to moderate persistent asthma (mild:9, moderate:26) from Huaxi Hospital asthma clinic were treated with combined medi-

Bronchial hyperreacfivity in endobronchial tuberculosis

Objective To investigate the bronchial hyperreactivity (BHR) and pulmonary function in patients suffering from endobronchial tuberculosis (EBTB) , and therefore to provide clues for early and correct diagnosis of EBTB. Methods Twenty-four patients with EBTB, proved by fiberoptic bronchoscopy, chest computerized tomography and biopsy, received measurements of pulmonary ventilatory function, airway resistance (Raw) , and