Changes and significance of the ratio of T helper 17 and regulatory T cell in peripheral blood of patients with coal-burning-borne arsenic poisoning

Objective To observe the change of T helper 17(Th17),regulatory T cell (Treg) as a percentage of lymphocytes and the Th17/Treg ratio in peripheral blood of patients with coal-burning-borne arsenic poisoning,and to explore the role of Th17 cells and Treg cells balance in arsenic-induced immune injury.

Differential expression and significance of Foxp3,TGF-β1 and IL-2 in peripheral blood of patients with coal-burning arsenic poisoning

91-95.Objective To investigate the expression of transcriptionfactor forkhead /winged helix transcription factor 3(Foxp3),immune factor transforming growth factor-beta1 (TGF-β1),and T-lymphocyte activation related factorinterleukin-2 (IL-2) in peripheral blood of patients withcoal-burning arsenic poisoning,and to analyze the effectsof arsenic exposure on immune function. Methods Acase-control study was conducted to investigate 149 cases[94 males and 55 females,(50. 69 ± 6. 14) years old]of arsenic poisoning in Yuzhang coal-burning arsenic poisoningarea,southwestern Guizhou Province,and the caseswere diagnosed based on the“Diagnosis of EndemicArsenicosis”(WS /T 211-2015) and confirmed by clinicalreview. According to skin damage,the patients weredivided into mild arsenic poisoning group (39 cases),moderate arsenic poisoning group (54 cases) and severearsenic poisoning group ( 56 cases );and 41 cases[12 males and 29 females,(45. 76 ± 7. 88) years old]of non-arsenic exposed residents from 12 km of Yuzhangcoal-burning area were selected as control group. Morningurine and peripheral blood samples were collectedwith informed consent.

Combined effects of fluoride and arsenite on the expression of Runx-related transcription 2 mRNA in bone of rats

Objective To explore the combined effects of fluoride and arsenite on the expression of Runx-related transcription 2(Runx2)mRNA in bone of Sprague Dawley(SD)rats.Methods Fifty four SD rats were selected[body mass(109.71±10.52)g,half male and half female].3×3 Factorial experimental design was used to evaluate the combined effects of fluoride and arsenite on the expression of Runx2 mRNA by random number table.

Effects of sodium arsenite on the expression of microRNA-191 and tissue inhibitor of metalloproteinase 3 in L-02 cells

Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-02 cells were exposed to different doses of Na2As O2[0（control group）,5,25,50 and 75μmol/L]

Combined effects of fluoride and arsenic exposure on proliferation,differentiation and beta-catenin expression in rat osteoblasts

<正>Objective To investigate the combined effects of fluoride(Na F)and arsenate(Na As O2)exposure on proliferation,differentiation and bata-catenin expression in SD rat osteoblasts.Methods Osteoblasts were isolated from calvarias of twelve SD rats born in 1~3 days and cul-