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Determination of Organochlorine Pesticide Residues in Pumpkin, Spinach and Sorrel Leaves Grown in Akwanga, Nasarawa State, Nigeria
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作者 E. G. Ibrahim N. Yakubu +1 位作者 L. Nnamonu J. M. Yakubu 《Journal of Environmental Protection》 2018年第5期508-515,共8页
Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from a farm in Akwanga and were tested for the presence of residues of organochlorine pesticides. The concentrati... Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from a farm in Akwanga and were tested for the presence of residues of organochlorine pesticides. The concentrations of all the pesticide residues in the vegetable samples were determined using GC/MS. Among the organochlorine pesticide p,p’-DDT was detected in pumpkin (0.75 mg/kg), spinach (0.319 mg/kg) and sorrel (0.219 mg/kg). θ-BHC and γ-BHC were detected only in pumpkin leaves (0.359 mg/kg and 0.647 mg/kg respectively). Dieldrin was detected in spinach and sorrel (0.124 mg/kg and 0.053 mg/kg respectively). Endrin was detected in pumpkin (0.732 mg/kg) and Aldrin in sorrel (0.095 mg/kg). All these values were above the maximum residue limit (MRL) value of the pesticides. Endosulfan II was detected in sorrel (0.306 mg/kg) below the MRL. The levels of most of the pesticide residues found in vegetables were above the maximum residue limits (MRLs) that call for laws to regulate the use and circulation of such chemicals. Routine monitoring of pesticide residues in this study area is necessary for the prevention, control and reduction of environmental pollution, to minimize health risks. 展开更多
关键词 Akwanga ORGANOCHLORINE PESTICIDE RESIDUES Maximum Residue Limit VEGETABLES
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Prevalence of Organophosphorous Pesticide Residues in Pumpkin, Spinach and Sorrel Leaves Grown in Akwanga, Nasarawa State, Nigeria
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作者 E. G. Ibrahim N. Yakubu +1 位作者 L. Nnamonu J. M. Yakubu 《Journal of Environmental Protection》 2018年第5期516-524,共9页
Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from three different locations in a farm in Akwanga and were tested for the presence of organophosphorus (OP) com... Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from three different locations in a farm in Akwanga and were tested for the presence of organophosphorus (OP) compounds. The concentrations of all the pesticide residues in the vegetable samples were determined using gas chromatography with mass spectrometry (GC-MS). The organophosphorus pesticides detected include carbaryl (0.052 mg/kg) in pumpkin, this is below European maximum residues limit (EU MRL) of 0.1 mg/kg, Dimethoate was found in pumpkin (0.165 mg/kg), spinach (0.103 mg/kg) and sorrel (0.250 mg/kg) all above the EU MRL of 0.05 mg/kg. Dichlofenthion was detected in pumpkin (0.308 mg/kg), pirimiphos methyl was detected in pumpkin and spinach (0.428 mg/kg and 0.149 mg/kg respectively), all these were below the EU MRL of 0.5 mg/kg. Chlorpyrifos was found in spinach (0.230 mg/kg) and sorrel (0.192 mg/kg) only spinach was above the EU MRL of 0.2 mg/kg. The pesticide residue Bromophosethyl was detected in all vegetables, pumpkin leaves (0.501 mg/kg), sorrel (1.571 mg/kg) and spinach (7.981 mg/kg) all above the EU MRL of 0.5 mg/kg. The remaining pesticides detected were all below their EU MRL value;these are Ethion found in spinach (0.167 mg/kg), Methyl Parathion in spinach (0.103 mg/kg) and sorrel (0.335 mg/kg). The levels of some of the organophosphorus pesticide residues found in vegetables were above the maximum residue limits (MRLs) set by the European Union. This calls for laws to regulate the use and circulation of such chemicals. Based on the observation made in these studies, it is proposed that more extensive monitoring investigation covering all vegetables part in Akwanga Nasarawa state be carried out to find the exact position of pesticide residues. 展开更多
关键词 Akwanga PESTICIDE RESIDUES ORGANOPHOSPHOROUS Maximum RESIDUE Limit
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亚马逊利什曼原虫无鞭毛体的体外培养及其抗原性分析 被引量:1
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作者 王华民 Soong Lynn 《中国热带医学》 CAS 2006年第5期755-756,810,共3页
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法,并用间接免疫荧光法分析其抗原特异性。方法小鼠皮损组织处获得的无鞭毛体(Al)、由前鞭毛体转化来的无鞭毛体(Ap)、J774.G8巨噬细胞来源的无鞭毛体(Aj)置于改良后的Schneider’sDrosophil... 目的建立亚马逊利什曼原虫无鞭毛体体外培养方法,并用间接免疫荧光法分析其抗原特异性。方法小鼠皮损组织处获得的无鞭毛体(Al)、由前鞭毛体转化来的无鞭毛体(Ap)、J774.G8巨噬细胞来源的无鞭毛体(Aj)置于改良后的Schneider’sDrosophila培养基,pH4.6、33℃条件下培养,取培养物光镜下观察并用间接免疫荧光法分析。结果三种不同来源无鞭毛体体外培养物光镜检查形态相似,均与纯培养获得的无鞭毛体一样。与无鞭毛体特异性蛋白P-8和P-4特异性单克隆抗体进行间接免疫荧光分析显示,三种不同来源的无鞭毛体均出现阳性,且浓度与部位相似。而前鞭毛体与抗P-8单克隆抗体(mAb)染色呈弱阳性反应,与抗P-4单克隆抗体呈阴性反应。相反,GP-46/M-2McAb与前鞭毛体染色呈强阳性反应,与无鞭毛体仅呈弱阳性反应。结论在33℃条件下,用改良的Schneider’sDrosophila培养基进行无鞭毛体体外培养获得成功。用阶段特异性单克隆抗体,进行间接免疫荧光分析法可鉴定亚马逊利什曼原虫的无鞭毛体。 展开更多
关键词 亚马逊利什曼原虫 无鞭毛体 体外培养 间接免疫荧光
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BrdU标记的人巨细胞病毒感染HEL细胞的方法学研究
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作者 傅鹰 陈利玉 +2 位作者 罗敏华 李建华 杨滔 《微生物与感染》 2006年第2期91-94,共4页
目的以标记在人巨细胞病毒(HCMV)DNA上的BrdU为示踪剂,研究病毒在受染HEL细胞中的移动过程;同时结合病毒蛋白pp65的表达探讨病毒复制、增殖的过程。方法以BrdU标记的HCMV(MOI=4)感染HEL细胞,分别选取感染后2h、4h、6h、24h及48h 5个时... 目的以标记在人巨细胞病毒(HCMV)DNA上的BrdU为示踪剂,研究病毒在受染HEL细胞中的移动过程;同时结合病毒蛋白pp65的表达探讨病毒复制、增殖的过程。方法以BrdU标记的HCMV(MOI=4)感染HEL细胞,分别选取感染后2h、4h、6h、24h及48h 5个时间点的细胞,用抗BrdU单克隆抗体,研究病毒核酸的胞内定位;同时用抗HCMV蛋白pp65的单克隆抗体检测此蛋白的表达及分布。结果免疫细胞荧光染色结果提示:在感染5个时间点,病毒DNA依次位于胞质、胞核及同时位于胞核和胞质;蛋白pp65的表达及分布规律为:胞内无表达、胞核分布、胞核与胞质同时分布及巨细胞和融合细胞内分布。结论以BrdU为标记物标记双链DNA病毒核酸不仅为研究HCMV.的胞内移动提供了良好的模型,同时也为其他病毒的研究提供了良好的工具;本实验结合HCMV蛋白pp65的表达和分布直观地反应了HCMV感染HEL细胞并在其中复制、增殖的过程。 展开更多
关键词 人巨细胞病毒 BRDU HEL细胞 PP65
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上海地区学生午餐营养及供应链状况研究
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作者 赵渝 RyanB.Zhao 《中国学校卫生》 CAS 北大核心 2003年第5期437-438,共2页
目的 了解上海地区学生午餐营养及供应链状况 ,为改善学生午餐营养状况 ,保障食品供应安全提供参考。方法 分析上海市学生午餐的营养、卫生、供应链、加工工艺等状况 ,采用定期抽检的方法 ,应用SNHAS对营养素、食品安全、感官进行综... 目的 了解上海地区学生午餐营养及供应链状况 ,为改善学生午餐营养状况 ,保障食品供应安全提供参考。方法 分析上海市学生午餐的营养、卫生、供应链、加工工艺等状况 ,采用定期抽检的方法 ,应用SNHAS对营养素、食品安全、感官进行综合评价。结果 各单位样本综合评价均合格 ,其中热量、蛋白质、脂肪均能达到或超过DRIs供给量的要求 ,但矿物质中Ca(仅为AI的 32 % )、视黄醇当量 (仅为AI的 57% )供给量较低。对供应链及卫生状况的分析表明 ,大型企业对各个环节控制严格 ,产品质量较好 ,但也存在保存时间过长等问题。结论 应进一步完善供应体系 ,建立高效的质量控制体系 ,推广营养配膳系统。 展开更多
关键词 学生保健服务 营养调查 评价研究 食品污染
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口蹄疫紧急疫苗研究进展(续完)
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作者 赵渝 Ryan B.Zhao 孙健和 《动物科学与动物医学》 2002年第9期28-31,共4页
关键词 口蹄疫 疫苗 病毒抑制 免疫动物 感染动物
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The Poly(vinyl alcohol)-Immobilized Photobacteria for Toxicology Monitoring
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作者 Kristina A. Alenina Leyla E. Aleskerova +1 位作者 Polina B. Kascheyeva Anvar D. Ismailov 《Engineering(科研)》 2012年第10期118-120,共3页
The present work is undertaken to studies the important factors affecting the stability of a light emission at immobilized photobacteria, and application PVAGs luminous biosensors for biomonitoring of toxicants. The i... The present work is undertaken to studies the important factors affecting the stability of a light emission at immobilized photobacteria, and application PVAGs luminous biosensors for biomonitoring of toxicants. The intensity and stability of a light emission is competently controlled by: 1) intensity and persistens of a luminescent cycle using bacterial strain;2) type of the carrier and the composition of the gel-formation medium;3) freeze-thawing procedures;4) physical and chemical conditions of storage and application. 展开更多
关键词 Component BIOLUMINESCENCE Photobacteria IMMOBILIZATION POLY (Vinyl Alcohol)
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印度中部的甲真菌病:临床病因的联系性
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作者 Garg A. Venkatesh V. +1 位作者 Singh M. 阎小宁 《世界核心医学期刊文摘(皮肤病学分册)》 2005年第1期30-31,共2页
Background. Onychomycosis is mainly caused by dermatophytes, but yeasts and nondermatophyte molds have also been implicated, giving rise to diverse clinical presentations. The etiological agents of the disease may sho... Background. Onychomycosis is mainly caused by dermatophytes, but yeasts and nondermatophyte molds have also been implicated, giving rise to diverse clinical presentations. The etiological agents of the disease may show geographic variation. The aim of the present study was to isolate the causative pathogens and to determine the various clinical patterns of onychomycosis in central India. Methods. The study population comprised 90 patients with onychomycosis. Nail samples were collected for direct microscopic examination and culture. Clinical patternswere noted and correlatedwith causative pathogens. Results. The male: female ratio was 3:1 and the mean age was 29.40 ±13.61 years. Fingernails were involved in 60%, toenails in 26.67%and both fingernails and toenails in 13.34%of the 90 patients. The clinical types noted were distolateral subungual onychomycosis (64.44%), total dystrophic onychomycosis (17.78%), proximal subungual onychomycosis with paronychia (12.2%), proximal subungual onychomycosiswith-out paronychia (4.44%) and superficial white onychomycosis (1.11%). Dermatophyteswere themost common pathogens isolated, being found in 24 patients (26.36%) [Tricophyton rubrum (23.07%), Tricophyton verrucosum(2.22%) and Epidermophyton floccosum (1.11%)], followed by Candida albicans, which was found in 22 patients (24.27%). Thirty-six (39.58%) nondermatophyte molds were isolaled from 29 patients. Of these 29 cases, six were associated with Tricophyton rubrum, which was considered the primary pathogen. Conclusions. Distolateral subungual onychomycosis was the most common clinical presentation; however, total dystrophic onychomycosis and proximal subungual onychomycosiswere not uncommonin this part of India. Tricophyton rubrum and Candida albicans were the major pathogens. The clinicoetiologic correlation revealed that a single pathogen could give rise to more than one clinical type. 2004 The International Society of Dermatology. 展开更多
关键词 甲真菌病 临床病因 皮肤癣菌 疣状毛癣菌 真菌培养 红色毛癣菌 下型 联系性 皮肤真菌 临床表型
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委内瑞拉妇女中HLA-DQB1与宫颈癌的相关性
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作者 Sierra-Torres C.H. Robazetti S.C. +2 位作者 S.K. Tyring 张家墅 程金湘 《世界核心医学期刊文摘(妇产科学分册)》 2005年第6期45-45,共1页
Cervical cancer represents a major health problem in Venezuela as well as in other Latin American countries. High- risk human papillomavirus (HR- HPV) infection is known as the major risk factor of cervical cancer. Ho... Cervical cancer represents a major health problem in Venezuela as well as in other Latin American countries. High- risk human papillomavirus (HR- HPV) infection is known as the major risk factor of cervical cancer. However, whether or not a HR- HPV- infected woman progresses to cervical cancer may depend on the immune system effectors induced by viral antigens presented by her specific human leukocyte antigens (HLA) alleles. The role of the HLA system in presenting peptides to antigenspecific T- cells may be critical for genetic susceptibility and genetic resistance to cervical carcinoma. We aimed to investigate the relationship between HLA- DQB1, HPV infection, and cervical cancer in Venezuelan women. Blood samples and cervical swabs were obtained from 36 patients and 79 healthy controls; additional cervical biopsies were obtained from all the patients. HPV DNA was detected by PCR and HLA- DQB1 genotyping was performed using a PCR- SSP protocol. A positive association with cervical cancer was observed for HLA- DQB1* 0201- 0202 and* 0402 alleles, however after Bonferroni correction only HLA- DQB1* 0402 remained statistically significant (P value = 0.004, RR = 5.067). This is the first report of HLA- DQB1 alleles associated with cervical carcinoma in Venezuelan women. Larger studies are needed to assess whether these HLA- DQB1* 0201- 0202 and* 0402 alleles have a direct effect on disease susceptibility. 展开更多
关键词 HLA-DQB1 妇女宫颈癌 人乳头瘤病毒 癌易感性 抗原特异性 细胞反应 遗传易感性 免疫应答反应 基因分型 人白细胞抗原
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