Migration of R28 Retinal Precursor Cells into Cochlear and Vestibular Organs

Damaged hair cells and neurons in the inner ear generally can not be replaced in mammals. The loss of these cells causes permanent functional disorders in both the cochlear and vestibular systems. Transplantation of retinal precursor cells, R28 cells, into inner ear tissue may help replace missing cells. The aim of the current project was to induce R28 cell transdifferentiation into cochlear and vestibular cell types under culture conditions. The first part was related to R28 cell labeling with DiI fluorescence that would help identify and track R28 cells. The second part involved co-culturing R28 cells in cochlear and vestibular organotropic cultures or isolated spiral ganglion neurons. The results suggest that R28 cells have the potential to differentiate into supporting cell types and spiral ganglion neurons in serum free medium, probably under the influence of diffusible signals from inner ear tissues. This information is useful for future efforts in inducing stem cell differentiation in the inner ear to replace lost sensory and neural cells.

光学相干断层成像术在玻璃体视网膜疾病诊治中的应用

光学相干断层成像术 (opticalcoherencetomogra phy ,OCT)是一种对眼部组织进行横断扫描 ,并具有高分辨力的新兴视网膜厚度成像技术。本研究将介绍这项技术的背景知识并且阐述OCT在正常黄斑及玻璃体后粘连、玻璃体 黄斑牵拉、黄斑视网膜裂孔、黄斑水肿、视网膜前膜、年龄相关性黄斑变性、视网膜脱离、脉络膜癌、眼肿瘤等眼科常见疾病中的图像表现特征 。

OBSERVATIONS ON THE EFFICACY IN TREATING SCLERITIS BY PLUM-BLOSSOM NEEDLE

Scleritis is a disease commonly seen inclinic.In traditional Chinese medicine,thecondition is considered to be related to theLungs.Based on this theory,we treated 28cases(29 eyes)of scleritis by the plum-blos-som needle with fairly good results.Pres-ented in this paper is a report of the 28 casesso treated.

In Vitro Model of Human Choroidal Neovascular

Choroidal capillary endothelia cell （CEC） plays a critical role in the development of choroidal neovascularization which is one of the major causes of blindness. An effective method for CEC cultivation was proposed. The isolation of human choroidal CECs using micro dissection followed by the use of superparamagnetic beads （Dynabeads） coated with the CD 31, which selectively binds to the endothelial cell surface. Cells bound to beads were isolated using a magnetic particle concentrator. The CECs were planted into type Ⅳ collagen coated 24 well plates. The results show that the primary cultured CEC is induced to tube formation in collagen Ⅳ coated environment, which can be presented as an in vitro model of choroidal neovascularization.

Assessment of Sulf hydryl Group in Individual Rat Lens Protein Subunits During Galactose Cataract Development

A specific reagent DACM [N-( 7-Dimethylamino-4-methyl-3-coumarinyl) maleimide] is used to study the -SH groups in lens proteins of normal and galactose cataractous rats. DACM when reacts readily with -SH groups form strong fluorescent adducts. The two -dimensional electrophoresis with DACM pre-labeled proteins is a simple and sensitive method for detecting -SH groups of protein subunit. In the present study, based on IEF/SDS-PAGE electrophoretically characterized soluble crystallins, describes specific ...

Use of Perfluorocarbon Liquids in Complicated Retinal Detachments

We now have an additional tool to help in managing complicated retinal detachments. We look forward to the day when we can leave the liquid without having to remove it because of the possibility of ocular complications.

维替泊芬光动力治疗后出现背痛

PURPOSE: To report the incidence of back pain after photodynamic therapy,which suggests methods for prevention that are related to its pathogenesis. DESIGN: Retrospective case series. METHODS:We retrospectively observed 548 patients who had undergone photodynamic therapy with verteporfin. RESULTS: Of 548 patients at the first treatment,14 patients (2.6% ) experienced pain during the infusion. Eleven patients were being treated for age-related macular degeneration; their mean age was 81 years,which significantly differed from the mean age of the overall age-related macular degeneration group (P=.003). The pain was mild in eight patients,moderate in four patients,and severe in two patients,with dyspnea and precordial pain. Five of the 14 patients had further courses of photodynamic therapy. After being treated prophylactically 60 minutes before photodynamic therapy,only one patient reported further mild pain. CONCLUSIONS: The biologicmechanisms of back pain may involve a high level of circulating thromboxanes that are induced by the liposomal composition of verteporfin. Prevention may include hydration,nonsteroidal anti-inflammatory drugs,and halving the infusion rate.

经巩膜固定人工晶体矫正无晶体眼的改良技术

Purpose. To examine the safety and efficacy of a modified scleral fixated intraocular lens (SFIOL) technique combining a scleral tunnel for intraocular lens (IOL) insertion, horizontal passage of sutures, and performing anterior vitrectomy through self-sealing pars plana sclerostomies. Methods. Retrospective reviewof all patients who underwent SFIOL for aphakic correction at the Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (a tertiary referral centre) between October 2000 and December 2001 with either the modified or conventional techniques. Results. A total of 20 eyes were identified. Eight modified (Group 1) and 12 conventional (Group 2) with a mean follow-up of 18.3 months. Postoperatively, all the eyes improved on unaided preoperative acuity and they all had a stable and well-positioned IOL. In all, 85% (7/8) of eyes in Group 1 maintained or improved best-corrected visual acuity. Conclusions. The modified SFIOL technique was found to be as safe and effective as conventional techniques. The main advantages of this technique include easy intraoperative suture management and superior globe maintenance.

血浆、红细胞和有或无白内障时晶状体的维生素E含量

Purpose: To compare levels of α - tocopherol in human lenses with cataract to clear human lenses and to determine associations between levels of α - tocopherol in plasma, red blood cells, and human lenses with cataract. Design: Crosssectional study. Methods: Concentrations of α - tocop- herol were measured by high- performance liquid chromatography in blood and in human lenses with and without cataract. Lenses were obtained during cataract surgery and from a regional eye bank. Peripheral α - tocopherol status in patients was assessed as plasma α - tocopherol (total and corrected for low- density lipoproteins) and as red blood cell bound α - tocopherol. Data (mean± standard error of the mean) are expressed as μ M α - tocopherol/g lens protein, μ Mα - tocopherol/l plasma, μ M α - tocopherol/g low- den- sity lipoproteins, and μ M α - tocopherol/T red blood cells. Results: Concentrations of α - tocopherolwere measured in 27 lenses of cataract patients, 8 cadaver lenses with cataract and in 14 clear cadaver lenses. The concentration of α - tocopherol was significantly higher in cataract than in control cadaver lenses (0.49± 0.04 vs 0.35± 0.03, P< .05). The difference between α - tocopherol in lenses of cataract patients and control cadaver lenses was even higher (0.7± 0.1 vs 0.35 ± 0.03, P< .01). No significant correlation was observed between plasma α - tocopherol or red blood cell bound α - tocopherol and lens α - tocopherol in patients. Conclusions: Serum and red blood cell levels of α - tocopherolmay not reflect the α - tocopherol status of the lens itself and therefore may not be clinically relevant markers for cataract risk. Mechanisms leading to increased levels of α - tocopherol in cataract lenses need to be explored in future research.