A synthetic metalloporphine was immobilized onto a PVA-based and mercapto-grafted solid support, emulating the active site of cytochrome P450. Its ligninolytic peroxidase-like catalytic activity was studied. The coord...A synthetic metalloporphine was immobilized onto a PVA-based and mercapto-grafted solid support, emulating the active site of cytochrome P450. Its ligninolytic peroxidase-like catalytic activity was studied. The coordinated mercapto ligand significantly affected the catalytic features of the catalyst because the oxidation of lignin-model compounds was very slow by comparison with imidazoleand pyridine-coordinated immobilized metalloporphines. Conversely, the catalyst efficiently bleached several industrial dyes and thus demonstrated promising activity for this application. Based on this altered substrate specificity the oxygen-donor catalytic route seems to be more favorable than a single electron oxidation pathway.展开更多
Background/Aims: T- lymphocyte reactivity against viral antigens may represent the only immunological marker of host contact with a virus. Aim of the present study was to investigate whether vertical exposure to hepat...Background/Aims: T- lymphocyte reactivity against viral antigens may represent the only immunological marker of host contact with a virus. Aim of the present study was to investigate whether vertical exposure to hepatitis C virus (HCV) could activate HCV- specific T- cell responses that may represent a biomarker of previous contact with the virus, and possibly contribute to the low rate of vertical HCV transmission. Methods: We studied 28 children born from chronically HCV- infected mothers. HCV- specific activation and proliferation of CD4+ lymphocytes and cytokine production were evaluated in cultures of peripheral blood mononuclear cells (PBMCs) stimulated in vitro with HCV- peptides. Results: HCV- specific CD4+ cell reactivity was observed in 20 out of the 28 children (71% ). The proliferation of HCV- specific CD4+ cells was more frequent and vigorous in children than in their mothers. In children, but not in the mothers, activation of CD4+ cells upon stimulation with HCV- peptides was directly correlated with proliferation. Early upon stimulation with HCV- peptides, lymphocytes from children produced lower levels of IL- 10 than lymphocytes from the mothers. Conclusions: Vertical exposure to HCV induces the development of viral- specific CD4+ cell- mediated immune responses, possibly endowed with protective function against infection, which may contribute to the low rate of vertical HCV transmission.展开更多
基金supported by the Regione Autonoma Sardegna (PO Sardegna FSE 2007-2013, L.R.7/2007, Bando Giovani Ricercatori, Project CRP1_27)
文摘A synthetic metalloporphine was immobilized onto a PVA-based and mercapto-grafted solid support, emulating the active site of cytochrome P450. Its ligninolytic peroxidase-like catalytic activity was studied. The coordinated mercapto ligand significantly affected the catalytic features of the catalyst because the oxidation of lignin-model compounds was very slow by comparison with imidazoleand pyridine-coordinated immobilized metalloporphines. Conversely, the catalyst efficiently bleached several industrial dyes and thus demonstrated promising activity for this application. Based on this altered substrate specificity the oxygen-donor catalytic route seems to be more favorable than a single electron oxidation pathway.
文摘Background/Aims: T- lymphocyte reactivity against viral antigens may represent the only immunological marker of host contact with a virus. Aim of the present study was to investigate whether vertical exposure to hepatitis C virus (HCV) could activate HCV- specific T- cell responses that may represent a biomarker of previous contact with the virus, and possibly contribute to the low rate of vertical HCV transmission. Methods: We studied 28 children born from chronically HCV- infected mothers. HCV- specific activation and proliferation of CD4+ lymphocytes and cytokine production were evaluated in cultures of peripheral blood mononuclear cells (PBMCs) stimulated in vitro with HCV- peptides. Results: HCV- specific CD4+ cell reactivity was observed in 20 out of the 28 children (71% ). The proliferation of HCV- specific CD4+ cells was more frequent and vigorous in children than in their mothers. In children, but not in the mothers, activation of CD4+ cells upon stimulation with HCV- peptides was directly correlated with proliferation. Early upon stimulation with HCV- peptides, lymphocytes from children produced lower levels of IL- 10 than lymphocytes from the mothers. Conclusions: Vertical exposure to HCV induces the development of viral- specific CD4+ cell- mediated immune responses, possibly endowed with protective function against infection, which may contribute to the low rate of vertical HCV transmission.
