Objective: To evaluate an association between cytokine production with common dietary proteins as a marker of non-allergic food hypersensitivity (NFH) and ga strointestinal (GI) symptoms in young children with autism ...Objective: To evaluate an association between cytokine production with common dietary proteins as a marker of non-allergic food hypersensitivity (NFH) and ga strointestinal (GI) symptoms in young children with autism spectrum disorders (ASD). Study design: Peripheral blood mononuclear cells (PB MCs)-were obtained from 109 ASD children with or without GI symptoms (GI [+] A SD, N = 75 and GI (-) ASD, N = 34], from children with NFH (N = 15), and contro l subjects (N = 19). Diarrhea and constipation were the major GI symptoms. We me asured production of type 1 T-helper cells (Th1), type 2 T-helper cells (Th2), and regulatory cytokines by PBMCs stimulated with whole cow’s milk protein (CM P), its major components (casein, β-lactoglobulin, and α-lactoalbumin), glia din, and soy. Results: PBMCs obtained from GI (+) ASD children produced more tu mor necrosis factor-α(TNF-α)/interleukin-12 (IL-12) than those obtained fr om control subjects with CMP, β-lactoglobulin, and α-lactoalbumin, irrespect ive of objective GI symptoms. They also produced more TNF-αwith gliadin, which was more frequently observed in the group with loose stools. PBMCs obtained fro m GI (-) ASD children produced more TNF-α/IL-12 with CMP than those from con trol subjects, but not with β-lactoglobulin, α-lactoalbumin, or gliadin. Cyt okine production with casein and soy were unremarkable. Conclusion: A high preva lence of elevated TNF-α/IL-12 production byGI (+)ASDPBMCs with CMP and its m ajor components indicates a role of NFH in GI symptoms observed in children with ASD.展开更多
文摘Objective: To evaluate an association between cytokine production with common dietary proteins as a marker of non-allergic food hypersensitivity (NFH) and ga strointestinal (GI) symptoms in young children with autism spectrum disorders (ASD). Study design: Peripheral blood mononuclear cells (PB MCs)-were obtained from 109 ASD children with or without GI symptoms (GI [+] A SD, N = 75 and GI (-) ASD, N = 34], from children with NFH (N = 15), and contro l subjects (N = 19). Diarrhea and constipation were the major GI symptoms. We me asured production of type 1 T-helper cells (Th1), type 2 T-helper cells (Th2), and regulatory cytokines by PBMCs stimulated with whole cow’s milk protein (CM P), its major components (casein, β-lactoglobulin, and α-lactoalbumin), glia din, and soy. Results: PBMCs obtained from GI (+) ASD children produced more tu mor necrosis factor-α(TNF-α)/interleukin-12 (IL-12) than those obtained fr om control subjects with CMP, β-lactoglobulin, and α-lactoalbumin, irrespect ive of objective GI symptoms. They also produced more TNF-αwith gliadin, which was more frequently observed in the group with loose stools. PBMCs obtained fro m GI (-) ASD children produced more TNF-α/IL-12 with CMP than those from con trol subjects, but not with β-lactoglobulin, α-lactoalbumin, or gliadin. Cyt okine production with casein and soy were unremarkable. Conclusion: A high preva lence of elevated TNF-α/IL-12 production byGI (+)ASDPBMCs with CMP and its m ajor components indicates a role of NFH in GI symptoms observed in children with ASD.
