The International Society of Reproductive Genetics(ISRG)assembled a workgroup made up of clinicians,clinical laboratory directors,and scientists for the purpose of creating the guidelines for preimplantation genetic t...The International Society of Reproductive Genetics(ISRG)assembled a workgroup made up of clinicians,clinical laboratory directors,and scientists for the purpose of creating the guidelines for preimplantation genetic testing(PGT).The most up-to-date information and clinical insights for the optimal PGT practice were incorporated in these guidelines.Recommendations are provided for embryologists,medical geneticists,clinical laboratorians,and other healthcare providers to improve the wellbeing of patients seeking assisted reproductive treatment and their offspring.展开更多
Carrier screening had been demonstrated as a powerful practice in preventing selected severe genetic disorders. This practice is expanding its scope and impact in the era of next-generation sequencing. Empirical and t...Carrier screening had been demonstrated as a powerful practice in preventing selected severe genetic disorders. This practice is expanding its scope and impact in the era of next-generation sequencing. Empirical and theoretical data support the utility of expanded carrier screening. The authors propose a comprehensive carrier screening program as a main component of the first-tier measure in preventing severe genetic disorders and birth defects in China. We discussed the key principles and important aspects to ensure the success of such a program. The authors believe this program will play a pivotal role in our endeavor for a healthier nation.展开更多
To the Editor:Noonan syndrome (NS)is a common genetic multisystem disorder caused by aberrant signal flux through mitogen-activated protein kinase (Ras-MAPK) and has an estimated prevalence of 1 in 1000-2500[1]. It wa...To the Editor:Noonan syndrome (NS)is a common genetic multisystem disorder caused by aberrant signal flux through mitogen-activated protein kinase (Ras-MAPK) and has an estimated prevalence of 1 in 1000-2500[1]. It was characterized by Jacqueline Noonan,who reported nine patients with pulmonary valve stenosis,small stature, hypertelorism,mild intellectual disability,webbed neck, undescended testes,and skeletal malformations[2], The lymphatic disorders are rare,it can happen at any age but most instances happen at birth,which are known to be particularly associated with NS,though it has not been well characterized to date.Gene mutations identified in individuals with the NS,regulate impertinently the Ras/ MAPK signal transduction pathway and they can currently explain 70 %of the NS cases.Therefore,it is very important for genetic counseling and life management.[2] Here,we reported a rare Noonan syndrome 9 patient in Asian with significant,persistent and progressive bilateral lower limb dysplasia.展开更多
Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic,causing serious infections.A key feature of S.aureus is its ability to acquire resistance to antibiotics.The presence of the staphylo...Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic,causing serious infections.A key feature of S.aureus is its ability to acquire resistance to antibiotics.The presence of the staphylococcal cassette chromosome(SCC) element in serotypes of S.aureus has been confirmed using multiplex PCR assays.The SCC element is the only vector known to carry the mecA gene,which encodes methicillin resistance in S.aureus infections.Here,we report the genome sequence of a novel methicillin-sensitive S.aureus(MSSA) strain:SCC-like MSSA463.This strain was originally erroneously serotyped as methicillin-resistant S.aureus in a clinical laboratory using multiplex PCR methods.We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S.aureus isolates.An open reading frame(CZ049;AB037671) was identified downstream of attL and attR inverted repeat sequences.Our results suggest that a lateral gene transfer occurred between S.aureus and other organisms,partially changing S.aureus infectivity.We propose that attL and attR inverted repeats in S.aureus serve as frequent insertion sites for exogenous genes.展开更多
The traditional approach of clinical genetic practice,known as the phenotype-first approach,usually starts with a thorough review of electronic medical records of the patient and a complete evaluation of patient's...The traditional approach of clinical genetic practice,known as the phenotype-first approach,usually starts with a thorough review of electronic medical records of the patient and a complete evaluation of patient's clinical presentation,as well as a survey of family history.Often more than one experienced clinical geneticists will order a variety of additional laboratory or imaging tests to obtain relevant information in order to reach a reasonable clinical diagnosis.Finally,molecular testing often helps to confirm the clinical diagnosis.This practice requires the availability of electronic medical records,well trained clinical geneticists who are able to perform relevant clinical evaluation,order appropriate tests and understand the molecular reports.These critical components of clinical genetics are mostly lacking in China today,1 as a consequence,most of patients with genetic condition do not receive a proper evaluation by clinical geneticists,only a small percent of patients received a clinical diagnosis and the majority remains undiagnosed for life.展开更多
基金National Key Research and Development Program of China(2021YFC2700701,2021YFC2701002,2020YFA0804000,2018YFC1004901)National Natural Science Foundation of China(82171677,81901495,82088102,81971344,82171686,82071661)+6 种基金Clinical Research Project of Shanghai Municipal Health Commission(202140110)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-064)International Science and Technology Collaborative Fund of Shanghai(18410711800)Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01)Shanghai Municipal Commission of Science and Technology Program(21Y21901002,22S31901500)Clinical Research Plan of SHDC(SHDC2020CR1008A)Shanghai Frontiers Science Research Base of Reproduction and Development,and Shanghai"Science and Technology Innovation Action Plan"Hong Kong,Macao,and Taiwan Science and Technology Cooperation Project(19410760100)
文摘The International Society of Reproductive Genetics(ISRG)assembled a workgroup made up of clinicians,clinical laboratory directors,and scientists for the purpose of creating the guidelines for preimplantation genetic testing(PGT).The most up-to-date information and clinical insights for the optimal PGT practice were incorporated in these guidelines.Recommendations are provided for embryologists,medical geneticists,clinical laboratorians,and other healthcare providers to improve the wellbeing of patients seeking assisted reproductive treatment and their offspring.
文摘Carrier screening had been demonstrated as a powerful practice in preventing selected severe genetic disorders. This practice is expanding its scope and impact in the era of next-generation sequencing. Empirical and theoretical data support the utility of expanded carrier screening. The authors propose a comprehensive carrier screening program as a main component of the first-tier measure in preventing severe genetic disorders and birth defects in China. We discussed the key principles and important aspects to ensure the success of such a program. The authors believe this program will play a pivotal role in our endeavor for a healthier nation.
文摘To the Editor:Noonan syndrome (NS)is a common genetic multisystem disorder caused by aberrant signal flux through mitogen-activated protein kinase (Ras-MAPK) and has an estimated prevalence of 1 in 1000-2500[1]. It was characterized by Jacqueline Noonan,who reported nine patients with pulmonary valve stenosis,small stature, hypertelorism,mild intellectual disability,webbed neck, undescended testes,and skeletal malformations[2], The lymphatic disorders are rare,it can happen at any age but most instances happen at birth,which are known to be particularly associated with NS,though it has not been well characterized to date.Gene mutations identified in individuals with the NS,regulate impertinently the Ras/ MAPK signal transduction pathway and they can currently explain 70 %of the NS cases.Therefore,it is very important for genetic counseling and life management.[2] Here,we reported a rare Noonan syndrome 9 patient in Asian with significant,persistent and progressive bilateral lower limb dysplasia.
基金supported by the National High Technology Research and Development Program (2006AA02Z4A9)the National Science and Technology Major Project of Ministry of Science and Technology of China (2009ZX10004,2012ZX10004206)the National Natural Science Foundation of China (30971610, 30900053)
文摘Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic,causing serious infections.A key feature of S.aureus is its ability to acquire resistance to antibiotics.The presence of the staphylococcal cassette chromosome(SCC) element in serotypes of S.aureus has been confirmed using multiplex PCR assays.The SCC element is the only vector known to carry the mecA gene,which encodes methicillin resistance in S.aureus infections.Here,we report the genome sequence of a novel methicillin-sensitive S.aureus(MSSA) strain:SCC-like MSSA463.This strain was originally erroneously serotyped as methicillin-resistant S.aureus in a clinical laboratory using multiplex PCR methods.We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S.aureus isolates.An open reading frame(CZ049;AB037671) was identified downstream of attL and attR inverted repeat sequences.Our results suggest that a lateral gene transfer occurred between S.aureus and other organisms,partially changing S.aureus infectivity.We propose that attL and attR inverted repeats in S.aureus serve as frequent insertion sites for exogenous genes.
文摘The traditional approach of clinical genetic practice,known as the phenotype-first approach,usually starts with a thorough review of electronic medical records of the patient and a complete evaluation of patient's clinical presentation,as well as a survey of family history.Often more than one experienced clinical geneticists will order a variety of additional laboratory or imaging tests to obtain relevant information in order to reach a reasonable clinical diagnosis.Finally,molecular testing often helps to confirm the clinical diagnosis.This practice requires the availability of electronic medical records,well trained clinical geneticists who are able to perform relevant clinical evaluation,order appropriate tests and understand the molecular reports.These critical components of clinical genetics are mostly lacking in China today,1 as a consequence,most of patients with genetic condition do not receive a proper evaluation by clinical geneticists,only a small percent of patients received a clinical diagnosis and the majority remains undiagnosed for life.