c-Jun dimerization protein JDP2 is a member of the AP-1 family that serves as a repressor in gene transcription. To study the role of JDP2 in neuroblastoma cells, we have established a stably trans- fected SH-SY5Y cel...c-Jun dimerization protein JDP2 is a member of the AP-1 family that serves as a repressor in gene transcription. To study the role of JDP2 in neuroblastoma cells, we have established a stably trans- fected SH-SY5Y cell line that carries jdp2 gene. Our results showed that JDP2 could promote neuronal differentiation of SH-SY5Y cells. The SY5Y-JDP2 cells exhibited lower percentage of cells in S phase and retarded growth rate than its parental cells that were accompanied by a higher expression of p21 and an intense reduction of the cyclin D1. In conclusion, the inhibitory role of JDP2 on neuroblastoma cell growth could be critical for the cells to be withdrawn from cell cycle and to proceed differentiation.展开更多
AIM:To isolate and characterize solid tumor inhibitory and other constituents from a bioactive extract of Casimiroa tetrameria((Rutaceae).METHODS:A crude extract of C.tetrameria obtained from the US National Cancer In...AIM:To isolate and characterize solid tumor inhibitory and other constituents from a bioactive extract of Casimiroa tetrameria((Rutaceae).METHODS:A crude extract of C.tetrameria obtained from the US National Cancer Institute Natural Product Repository and found to exhibit selective toxicity to solid tumor cells was subjected bioactivity-guided fractionation involving solvent-solvent partitioning,gel filtration,and chromatography.The structures of all isolated compounds were elucidated by spectroscopic analysis(NMR and MS) and/or by comparison with the reported data.Compounds 1 and 4-9 were evaluated for their solid tumor selective cytotoxicity.RESULTS:Nine metabolites,including a new furanocoumarin,5-methoxy-8-(4'-acetoxy-3'-methylbut-2-eny-loxy)-psoralen(1),and the previously known compounds 2-9 were encountered.Of these the flavonoid zapotin(6),and N-benzoyltyramide derivatives 7 and 8 were found to be the active constituents.CONCLUSION:Zapotin(6) is the most potent constituent of C.tetrameria with solid tumor selectivity.展开更多
基金the National Natural Science Foundation of China (Grant Nos. 90408007 and 30470376)
文摘c-Jun dimerization protein JDP2 is a member of the AP-1 family that serves as a repressor in gene transcription. To study the role of JDP2 in neuroblastoma cells, we have established a stably trans- fected SH-SY5Y cell line that carries jdp2 gene. Our results showed that JDP2 could promote neuronal differentiation of SH-SY5Y cells. The SY5Y-JDP2 cells exhibited lower percentage of cells in S phase and retarded growth rate than its parental cells that were accompanied by a higher expression of p21 and an intense reduction of the cyclin D1. In conclusion, the inhibitory role of JDP2 on neuroblastoma cell growth could be critical for the cells to be withdrawn from cell cycle and to proceed differentiation.
基金supported by the US National Institutes of Health research grant CA092143 awarded by National Cancer Institute
文摘AIM:To isolate and characterize solid tumor inhibitory and other constituents from a bioactive extract of Casimiroa tetrameria((Rutaceae).METHODS:A crude extract of C.tetrameria obtained from the US National Cancer Institute Natural Product Repository and found to exhibit selective toxicity to solid tumor cells was subjected bioactivity-guided fractionation involving solvent-solvent partitioning,gel filtration,and chromatography.The structures of all isolated compounds were elucidated by spectroscopic analysis(NMR and MS) and/or by comparison with the reported data.Compounds 1 and 4-9 were evaluated for their solid tumor selective cytotoxicity.RESULTS:Nine metabolites,including a new furanocoumarin,5-methoxy-8-(4'-acetoxy-3'-methylbut-2-eny-loxy)-psoralen(1),and the previously known compounds 2-9 were encountered.Of these the flavonoid zapotin(6),and N-benzoyltyramide derivatives 7 and 8 were found to be the active constituents.CONCLUSION:Zapotin(6) is the most potent constituent of C.tetrameria with solid tumor selectivity.
