Human bone allografts present a better alternative to autografts in terms of minimization of the harvesting procedure complications.Prior to the use in clinical applications,they require sterilization which aims to re...Human bone allografts present a better alternative to autografts in terms of minimization of the harvesting procedure complications.Prior to the use in clinical applications,they require sterilization which aims to reduce bioburden.This often comes at the expense of their biological properties as carriers of cells.In this study,we evaluated the cytocompatibility of human bone allografts processed and sterilized by three different methods withmesenchymal stromal cells.Bone morphology,biological and biochemical properties of the extracted bone-conditionedmedium and viability of cells were assessed.We found that chemical sterilization had a strong negative effect on cell viability,whereas thermal sterilization and washing with subsequentγ-irradiation both resulted in a bone graft compatible with the progenitor cells.Moreover,washing of the bone prior to sterilization allowed solid removal of cell debris and other bone marrow components.Taken together,our findings demonstrate the importance of a proper choice of the bone graft processing method for the production of the biomaterial suitable for tissue engineering.展开更多
基金the Ministry of Health of the Russian Federation[grant no 115030510010].
文摘Human bone allografts present a better alternative to autografts in terms of minimization of the harvesting procedure complications.Prior to the use in clinical applications,they require sterilization which aims to reduce bioburden.This often comes at the expense of their biological properties as carriers of cells.In this study,we evaluated the cytocompatibility of human bone allografts processed and sterilized by three different methods withmesenchymal stromal cells.Bone morphology,biological and biochemical properties of the extracted bone-conditionedmedium and viability of cells were assessed.We found that chemical sterilization had a strong negative effect on cell viability,whereas thermal sterilization and washing with subsequentγ-irradiation both resulted in a bone graft compatible with the progenitor cells.Moreover,washing of the bone prior to sterilization allowed solid removal of cell debris and other bone marrow components.Taken together,our findings demonstrate the importance of a proper choice of the bone graft processing method for the production of the biomaterial suitable for tissue engineering.
