The global registry of COVID-19 clinical trials: indicating the design of traditional Chinese medicine clinical trials

Objective:To analyze the registration information of Corona Virus Disease 2019(COVID-19)related clinical trials from all the clinical trial registry accepted by the International Committee of Medical Journal Editors(ICMJE).Methods:All the database of ICMJE-accepted clinical trial registry platform were searched for COVID-19 related clinical trials that registered from December 8,2019 to February 19,2020.Results:All the database totally contained data of 209 COVID-19 related clinical trials,including 66(31.6%)traditional Chinese medicine(TCM)related studies and 143(68.4%)non-TCM related studies.176(84.2%)clinical trials were registered in ChiCTR,as 33(15.8%)were registered in ClinicalTrials.gov.There were 152(72.7%)intervention trials,including 9(4.3%in total)preventive trials,136(65.1%in total)therapeutic trials and 7(3.3%in total)convalescent trials.Conclusion:At the present stage,clinical studies are still aiming at exploring the effective treatment of COVID-19,and the existing treatments need more high-quality clinical evidence supporting.Traditional Chinese medicine may have played an important role in all stages of the epidemic preventing and control,and the enthusiasm of carrying out scientific research has also been greatly improved.The relevant policies of clinical trial registration should be popularized among researchers,including traditional Chinese medicine researchers.And the methodological quality of clinical trials’design urges improving.

Clinical experience of treating diabetes and its complications with mulberry plants by Chinese medicine master Lv Ren-he

Lv Renhe,a master of Chinese medicine,was familiar with the sources of medicinal materials,the four nature and five flavors,and the characteristics of ascending,descending,floating and sinking.The study of medicine in clinical practice reflects the principle of“using medicine as a soldier”.In the treatment of diabetes mellitus and its complications,Professor Lv made good use of mulberry leaves,mulberry branches,mulberry white bark,mulberry and mulberry parasitism,and gave full play to the modern pharmacological effects of mulberry drugs such as hypoglycemic,hypotensive and lipidemic in the combined use,and achieved satisfactory results.

Study on the relationship between knee injury recovery and traditional Chinese medicine constitution of young male amateur cyclist in Hainan island cycling league

Objective:To study the relationship between the recovery of knee joint injury and traditional Chinese medicine constitution of young male amateur cyclist in Hainan.Methods:720 young male amateur cyclists were selected from the tour nanli lake national wetland park cycling race and Hainan international tourism island cycling league(ding'an station)in 2019.According to the classification criteria of TCM constitutions in classification and determination of TCM constitutions revised by the Chinese association of traditional Chinese medicine in April 2009,the TCM constitutions were divided into mild constitution group and Yin deficiency constitution group.Standard Kujala knee pain questionnaire was used to evaluate knee joint function in pinyin and Yin deficiency groups.To observe the relationship between TCM constitution and functional injury and recovery degree of knee joint.Results:Body mass index(BMI)of Yin deficiency quality was lower than that of pinyin deficiency quality(P<0.01).Score of knee flexion and extension degree was higher in Yin deficiency quality 30 minutes before the race(P<0.05).The score of Yin deficiency quality was lower after 30 minutes of squatting(P<0.01)and jumping(P<0.05).The total score of knee joint was higher 5 days after the race(P<0.01).The knee flexion and extension(P<0.05)and jump(P<0.05)were higher in the pre-race and post-race scores.Conclusion:There is a high correlation between the constitution of traditional Chinese medicine and the degree of functional injury and recovery of knee joint.The degree of post-game sports injury is higher and the recovery of post-game sports injury is faster.Therefore,different sports protection schemes can be formulated for different constitutions in bicycle RACES to reduce the occurrence of sports injuries and accelerate the recovery time of sports injuries.

The efficacy and safety of Chinese herbal medicine combined with ACEI/ARB fortreatment of incipient diabetic nephropathy： Ameta-analysis

目的：本研究评估中草药联合血管紧张素转换酶抑制剂（ACEIs）和/或血管紧张素受体阻滞剂（ARBs）治疗糖尿病肾病（IDN）的有效性和安全性.方法：在9个数据库中搜索中药（CHM）和ACEI/ARB治疗IDN的随机对照试验.纳入的文章发表于2006年1月至2016年12月.所有研究分为含黄芪和生地黄（i亚组）,含黄芪或生地黄（地黄）（ii亚组）,不含黄芪和地黄（iii亚组）.Review Manager5.3用于亚组分析.结果：最终纳入28个随机对照试验,共2017个患者.Meta分析结果显示：1）CHM联合ACEI/ARB治疗IDN与单用ACEI/ARB相比,尿白蛋白排泄率（UAER）显著降低,且i亚组降低UAER的效果优于另外2个亚组;2）CHM联合ACEI/ARB治疗IDN与单用ACEI/ARB相比,可显着降低血清肌酐（Scr）水平,且ii亚组降低Scr的效果优于iii亚组;3）CHM联合ACEI/ARB治疗IDN与单用ACEI/ARB相比,在降低BUN方面无统计学差异.结论：与单独应用ACEI/ARB相比,CHMs联合ACEI/ARB可显着降低UAER和Scr水平,且含黄芪或地黄的CHM亚组效果更为显著.故早期糖尿病肾病的治疗采用中草药联合ACEI/ARB是更为有效的治疗方法,同时应重视黄芪和地黄的应用.

Treatment Principles of Obesity with Chinese Herbal Medicine: Literature Analysis by Text Mining

Obesity represents a social health problem worldwide, associated with serious health risks and increased mortality. The prevalence of obesity is reported to be increasing in both developed and developing countries. Obesity is associated with a significant range of comorbidities and is linked with increases in mortality, thus the treatment of obesity is very important. Chinese herbal medicine (CHM) has been used for weight management both in China and in western countries for many years, the effectiveness and safety of CHMs in obesity have been proved. Yet the principles of treating obesity with CHMs are hard to manage due to the complexity of TCM theory. In this study, a novel text mining method was developed based on a comprehensive collection of literatures in order to explore the treatment principles more intuitively. Networks of TCM patterns and CHMs which are most frequently used in obesity treatment are built-up and analyzed, two major principles are explored in treating obesity: one is resolving phlegm and dampness, the other is clearing heat and reinforcing deficiency. These findings might guide the clinicians in treatment of obesity.

Clinical research progress of ischemic heart failure and prospect of traditional Chinese medicine

缺血性心力衰竭作为心力衰竭最常见的类型之一,如何诊疗始终为临床关注的重点。近年来,对于生物标志物的研究为临床诊断、病理分层和评价预后提供了帮助。常规治疗方面,新药物的临床应用及非药物治疗方式的发展带来了更多选择。同时,在中医辨证理论的指导下,中医药治疗也发挥着越来越重要的作用,展现了广阔前景。

Prospects for the future of clinical efficacy evaluation of traditional Chinese medicine——Emphasis on original theory and clinical practice

Traditional Chinese medicine(TCM)clinical efficacy evaluation runs through the development of TCM and has been progressing so far.With the introduction of evidence-based medicine(EBM)in recent 20 years,TCM clinical efficacy evaluation has developed rapidly,accumulated research and experience,improved the quality and standardization of research,but also gradually found deep-seated deficiencies.In 2019,the China Center for Evidence-based Traditional Chinese Medicine(CCEBTCM)was established,and TCM was included in the 11th revision of the International Statistical Classification of Diseases(ICD-11).In above opportunities for TCM clinical efficacy evaluation,it is necessary to face up to the previous deficiencies,so that the TCM clinical efficacy evaluation can truly reflect the actual clinical practice characteristics,including TCM clinical problems,TCM thinking and TCM diagnosis&treatment mode.Based on the summary and analysis of past research,development concepts are proposed in this article.On the one hand,original concepts of TCM should be confidently paid more attention to the design and implementation of TCM clinical efficacy evaluation to be close to TCM clinical practice.On the other hand,it is necessary to consolidate the foundation of TCM clinical practice before evaluating efficacy.Evaluated object built on TCM ontology knowledge should be demonstrated based on clinical practice,and unknown evaluated object combined with new methods,technologies and concepts should be maturely integrated in clinical practice firstly.

Risk assessment models of integrative medicine indicators for malignant transformation of chronic atrophic gastritis:A registry study protocol

Objective:Traditional Chinese medicine(TCM)and modern medicine have both been used in arresting malignant transformation of chronic atrophic gastritis(CAG)in China with good therapeutic effect.However,no studies have been undertaken to assess the risk of malignant transformation in CAG patients using both modern medicine and TCM features.Our study aimed to develop risk assessment models for malignant transformation of CAG combining indicators of both TCM and modern medicine.These models will facilitate early warning and control of malignant transformation of CAG from the perspective of evidence-based integrative medicine.Methods:In the proposed registry study,a total of 1000 eligible CAG patients will be recruited from four hospitals in China.A 10-year follow-up study will be conducted both on-site and off-site to track the events of malignant transformation.Frequency analysis and chi-squared tests will be used to perform the comparative analysis on the prevalence of malignant transformation events and indicators in TCM or modern medicine in different groups.A multivariate Cox proportional hazard model will be used to perform correlation analyses of malignant transformation events and factors of TCM or modern medicine.Conclusion:The proposed study has been designed with a large sample size and long follow-up period,in which wide-ranging modern medicine and TCM indicators can be gathered over the whole process of malignant transformation of CAG.Based on this study,risk assessment models for malignant transformation ofCAGmaybe constructed fromthe perspective of integrative medicine.This may provide clinicians and patients with an optimized early warning system as well as prevention strategies for malignant transformation of CAG.

The Chinese herbal formula Tongluo Jiunao promotes expression of brain-derived neurotrophic factor/tropomyosin-related kinase B pathways in a rat model of ischemic brain injury

The neurotrophin-Trk receptor pathway is an intrinsic pathway to relieve damage to the central nervous system. The present study observed the effects of Tongluo Jiunao （TLJN）, which comprises Panax Notoginseng and Gardenia Jasminoides, on expression of brain-derived neurotrophic factor （BDNF） and tropomyosin-related kinase B （TrkB） in a rat model of focal cerebral ischemic injury. Xue Sai Tong （XST）, comprising Panax Notoginseng, served as the positive control. Mechanisms of neuroprotection were analyzed following TLJN injection. Following establishment of the middle cerebral artery occlusion models, TLJN and XST were intraperitoneally injected, and 2, 3 5-triphenyltetrazolium chloride staining results revealed that TLJN injection reduced infarct volume, suggesting that TLJN exerted a neuroprotective effect. Enzyme-linked immunosorbent assay results showed that TLJN elevated BDNF and growth associated protein-43 expression in ischemic brain tissues, as well as serum BDNF levels. Reverse-transcription polymerase chain reaction and western blot results showed that TLJN injection did not affect TrkB expression in the ischemic brain tissues of rats. These results suggested that TLJN injection reduced damage to ischemic brain tissues and increased BDNF expression. In addition, TLJN injection resulted in better promoting effects on neurotrophic factor expression compared with XST.

Clinical relevance of Chinese syndrome factors and thromboelastography levels in patients with phlegm-heat and fu-organ excess syndrome

Objective:Syndrome differentiation is a unique part of traditional Chinese medicine(TCM).Syndrome factors play an important role in the diagnosis and treatment of TCM syndromes.Thromboelastography(TEG)intuitively reflects the blood status of patients with acute ischemic stroke(AIS)and is important in the treatment and prognosis of AIS.To identify the relationship between TCM syndrome factors and TEG in AIS patients and standardize TCM syndrome differentiation and treatment objectives,we designed a prospective cohort study of 103 AIS patients.Methods:We used the diagnostic criteria for AIS in the Chinese Guideline for Diagnosis and Management of Acute Ischemic Stroke 2010.Diagnosis of phlegm-heat and fu-organ excess syndrome was based on the TCM Scale for the Syndrome of Phlegm-heat and fu-organ Excess.The ischemic Stroke TCM Syndrome Factor Diagnostic Scale was used to identify and diagnose syndrome factors.General information,scores of syndrome factors and values of TEG parameters of all enrolled patients were recorded.Results:There were significant differences in internal fire and phlegm-dampness scores between patients with and without phlegm-heat and fu-organ excess syndrome(P<.05).In patients with phlegm-heat and fu-organ excess syndrome,internal fire was negatively correlated with TEG parameters R and K(P<.05)and positively correlated with alpha Angle and coagulation index(P<.01).There were no significant correlations between the two syndrome factors and MA(P Z.058)and LY30(P>.05)or between both syndrome factors and TEG parameters in patients without phlegm-heat and fu-organ excess syndrome.Conclusion:The syndrome factor internal fire is a potential predictor of increased platelet function and fibrinogen activity in AIS patients with phlegm-heat and fu-organ excess,and a potentially important predictor of blood hypercoagulability in TCM.

Efficacy and safety of Shenshuaining granule combined with Western medicine treatment intreatment of diabetic kidney disease: A meta- analysis

Objective:To systematically review the efficacy and safety of Shenshuaining granule combined with Western medicine treatment for Diabetic kidney disease(DKD)by meta-analysis.Methods:Databases including CNKI,WanFang Data,VIP,CBM,TheCochrane,Embase and PubMed were electronically searched from inception to May 2020 to collect randomized controlled trials of Shenshuaining granule with western medicine treatment for Diabetic nephropathy.The trial was screenedbased on inclusion and exclusion criteria,and the methodological quality of the included relevant trial was assessed by Cochrane Handboo-k.Meta-analysis was then performed by Revman 5.3 software.Results:A total of 9 studies involving 892 patients were included.Both groupinclude 446 patients.The result of meta-analysis showed that Shenshuaining granule combined with Western medicine treatment significantly improved the total effective rate of diabetic nephropathy[RR=1.26,95%CI(1.17,1.37),P<0.00001],further reduced ser umurea nitrogen[MD=-1.65,95%CI(-2.53,-0.77),P=0.0002],24h urine albumin excretion rat[SMD=-4.21,95%CI(-6.23,-2.19),P<0.0001],serum creatinine[MD=-21.50,95%CI(-28.48,-14.50),P<0.00001].However,the difference of fasting blood glucose[MD=-0.46,95%CI(-1.18,0.27),P=0.22]was not statistically significant.Conclusions:Shenshuaining granule can improve the rate o-f renal pellet filtration in patients with diabetic nephropathy,reduce kidney injury and protect kidney function.Although there are sideeffects,it is relatively safe.

The roles of traditional Chinese herbal medications in regulating mitochondrial activity to reverse cancer

Cancer was gradually regarded as a metabolic disease,which might be linked to impairment of mitochondrial function.There are really some essential differences between the mitochondria of normal and cancer cells,which might become promising target for cancer chemotherapy.According to western pharmacology,a known target was needed for drug development.Unfortunately,the number of therapeutic agents relating to the mitochondrion are severely limited because of lacking of knowledge about mitochondrial biology.Unlike the“Western Medicine”,some traditional Chinese herbal medicine(TCHM)might be prone to targeting mitochondria despite the lack of precise molecular mechanisms.If it is the case,we might be able to screen and identify active anti-cancer drugs from traditional Chinese therapeutics by using mitochondrial functional assay.In this review,we would give an overview of the effect of TCHM on the mitochondrion,and the challenges and breakthroughs remaining in cancer treatment.

Efficacy and safety of Shen Zhi Ling oral liquid(a Chinese patent)for Alzheimer's disease:A meta-analysis of randomized controlled trials

Objective:To evaluate the efficacy and safety of Shen Zhi Ling oral liquid(SZL)alone or combined with basic treatment in treating Alzheimer's Disease(AD).Methods:Databases including PubMed,the Cochrane Library,Embase,Web of science,China National Knowledge Infrastructure(CNKI),Wanfang Database and the Chinese Science and Technology Journal Database(VIP)were searched from inception to September 20,2020 to collect relevant randomized controlled trials(RCTs)comparing SZL with other nootropic drugs for AD.The primary clinical outcomes were scores of the Mini Mental State Examination(MMSE)and the Activities of Daily Living(ADL)during treatment.The secondary outcomes of our study included other neuropsychological scale scores including Rapid Verbal Retrieve(RVR),Digit Span(DS),Fuld Object-Memory Evaluation(FOM)and adverse events or reactions.Two authors searched and screened the literature independently,then extracted the data and evaluated the methodological quality by using the handbook of Cochrane Collaboration,and analyzed the data via the Review Manager 5.3 software.Results:In total,we included four studies,covering 217 participants.All studies were in a low methodological quality.The result of MMSE score,ADL score,RVR score,DS score and FOM score in the meta-analysis showed that no statistically significant difference(P>0.05)between the treatment groups and control groups,and the statistical heterogeneity of all meta-analyses was small(I2<50%).SZL was indicated no better than other nootropic drugs.Among the included studies,no study described adverse events.Conclusions:Considering the low quality of the included trials and the different levels of AD patients,we are unable to come to any conclusion about the efficacy and safety of SZL compared with other nootropic drugs.Moreover,we still need rigorously designed,multi-center,large-scale trials to further confirm the efficacy and safety of SZL.

Preparation of magnetic resonance probes using one-pot method for detection of hepatocellular carcinoma

AIM: To prepare the specific magnetic resonance(MR) probes for detection of hepatocellular carcinoma(HCC) using one-pot method.METHODS: The carboxylated dextran-coated nanoparticles were conjugated with anti-α-fetoprotein(anti-AFP) or anti-glypican 3(anti-GPC3) antibodies through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide(EDC/NHS)-mediated reaction to synthesize the probes.The physical and chemical properties of the probes were determined by transmission electron microscopy(TEM) and dynamic light scattering, and the relaxivity was compared to uncombined ultrasmall superparamagnetic iron oxide nanoparticles(USPIONs) using a 1.5T clinical MR scanner.The binding efficiency of the antibodies to nanoparticles was measured with an ultravioletvisible spectrophotometer.In addition, the probes were incubated with targetable cells in vitro.RESULTS: The superparamagnetic MR probes(antiGPC3-USPION probe and anti-AFP-USPION probe) were synthesized using one-pot method.Their mean hydrodynamic diameter was 47 nm with a broader slight size distribution.The coupling efficiency of carboxylated dextran-coated ultrasmall superparamagnetic iron oxide(USPIO) with anti-GPC3 or anti-AFP antibody was 15.9% and 88.8%, respectively.Each of the USPIO nanoparticles may bind 3 GPC3 antibodies or 12 AFP antibodies.The statistical analysis showed no significance(P > 0.05) in shortening the T1 and T2 values when comparing the USPIO-AFP or USPIOGPC3 to USPIO.Analysis of TEM images revealed that anti-GPC3-USPION probes and anti-AFP-USPION probes could specifically enter into the Hep G2 cell by combining with the GPC3 receptors or AFP receptors, whereas the Hep G2 cell sample incubated with USPIONs showed no or few nanoparticles in the cytoplasm.CONCLUSION: The synthesized probes using one-pot method can be used for in vitro experimental study and have potential clinical application in MR imaging for detection of hepatocellular carcinomas.

Effects of Yishendaluo decoction on blood-brain barrier integrity in mice with experimental autoimmune encephalomyelitis

This study investigated the effects of Yishendaluo decoction on the loss of blood-brain barrier integrity in mice exhibiting experimental autoimmune encephalomyelitis.To this end,we used real-time fluorescent quantitative PCR to measure the levels of mRNAs specific to the T cell markers CD4 and CD8,and the monocyte marker CD11b.In addition,we used Evans blue dye extravasation in the spinal cord and brain tissues to assess blood-brain barrier permeability.The results indicated that an increase in blood-brain barrier permeability was associated with an increase in CD4,CD8 and CD11b mRNA expression in experimental autoimmune encephalomyelitis mice.Yishendaluo decoction administration significantly reversed inflammatory cell accumulation in cerebral tissues of experimental autoimmune encephalomyelitis mice.

Preparation of Polyporus Albicans Teng Sulfate andIts Anti-coagulation Activity

More studies have indicated that polysaccharide sulfate has anti-coagulant activity.Now,heparin is the most popular anticoagulant used in clinic,however,its side effects have also caused highly concern.It is still under intensive investigations to synthesize effective safe polysaccharide sulfate as heparin substitute.We extracted water-soluble polysaccharide from fermented mycelium of edible polyporus albicans（Imaz.） teng,and got the water-soluble polyporus albicans teng sulfate（PATS） by modifying the water-solubility polyose with the method of chlorosulfonic acid-pyridine.The anti-coagulant assay of PATS in vitro towards normal human plasma indicates its remarkable anticoagulant activity,while the dose could be as low as 5 mg/L for anticoagulation.The anti-coagulant effect was equivalent to that of heparin about 150 U when the concentration of PATS was 10 mg/L.The study on anti-coagulation mechanism suggests that PATS got involved in the intrinsic pathway.The anti-coagulation activity of PATS was due to the inhibition of the coagulation factors IIa and Xa activities mediated by antithrombin Ⅲ（ATIII）.The anti-coagulation mechanism of PATS is absolutely identical to that of heparin.In conclusion,we suggest that PATS has the similar anti-coagulation characteristic to heparin,but with a better anti-coagulation effect.Meanwhile,derived from edible fungus-polysaccharide,PATS has more bio-safety advantage.Therefore,PATS has promising future to be developed and used as an ideal substitute for heparin in clinic.

Chinese herbal formula Tongluo Jiunao injection protects against cerebral ischemia by activating neurotrophin 3/tropomyosin-related kinase C pathway

The Chinese herbal formula Tongluo fiunao, containing the active components Panax notogin- seng and Gardenia jasminoides, has recently been patented and is in use clinically. It is known to be neuroprotective in cerebral ischemia, but the underlying pathway remains poorly understood. In the present study, we established a rat model of cerebral ischemia by occlusion of the middle cerebral artery, and administered Tongluo Jiunao, a positive control （Xuesai Tong, containing Panax notoginseng） or saline intraperitoneally to investigate the pathway involved in the action of TongIuo fiunao injection. 2,3,5-Triphenyltetrazolium chloride （TTC） staining showed that the cerebral infarct area was significantly smaller in model rats that received Tongluo fiunao than in those that received saline. Enzyme-linked immunosorbent assay revealed significantly greater expression of neurotrophin 3 and growth-associated protein 43 in ischemic cerebral tissue, and serum levels of neurotrophin 3, in the Tongluo Jiunao group than in the saline group. The reverse transcription polymerase chain reaction and immunohistochemical staining showed that after treatment with Tongluo fiunao or Xuesai Tong, tropomyosin-related kinase C gene expression and immunoreactivity were significantly elevated compared with saline, with the greatest expression observed after Tongluo Jiunao treatment. These findings suggest that Tongluo Jiunao injection exerts a neuroprotective effect in rats with cerebral ischemia by activating the neurotrophin 3/ tropomyosin-related kinase C pathway.

The effects of health Qigong Baduanjin on the prevention,treatment,and recovery of COVID‑19

To summarize the application and modern studies on health Qigong Baduanjin by domestic and foreign scholars,combined them with clinical practice of mobile hospital in Wuhan,we explore the prevention,treatment,and recovery of Baduanjin in the war against COVID‑19.On the basis of studying ancient classics,such as Huangdi’s Internal Classic,and the concrete exercise methods of Baduanjin,we propose three ideas:prevention before disease onset,promoting healing during illness,and strengthening the body after recovery.We aimed to give full play to Baduanjin in the battle against COVID‑19 to popularize correct prevention and health knowledge for the patients and the healthy as well as to provide a reference for medical workers to use Traditional Chinese Medicine to prevention and treatment.

Yiqi Huoxue prescription can prevent and treat post-MI myocardial remodeling through promoting the expression of AMPK signal pathway

Objective:To investigate how Yiqi Huoxue (YQHX) prescription regulates mitochondrial biosynthesis and ATP synthesis via AMP-activated protein kinase (AMPK) and to reveal its molecular mechanism in preventing and treating post-MI myocardial remodeling.Methods:The MI animal model of myocardial infarction were established by ligating Sprague Dawley (SD) rats' left anterior descending coronary arteries;the animals were randomly divided into MI group,YQHX prescription group and perindopril group,and a sham operation group was set at the same time.Related drug intervention was administered on the 2nd day after surgery,the YQHX group was given Astragalus,angelica,ginseng,Ligusticum wallichii and pseudoginseng,provided by the Dongzhimen Hospital of Beijing University of Chinese Medicine,once per day,at a dose of 21 g/kg body weight/day (the clinical equivalent dose based on a previous study),and the changes in relevant indicators were observed at 1 week and 4 weeks.Echocardiography (ECG) was used to observe the changes in rat cardiac structure and functions;the morphology-based technique was used to observe the changes in myocardial cells and mitochondria;the expression of AMPK signal pathway-related proteins and mRNA was detected using western blotting and real-time fluorescence quantification respectively,while fluorescence enzyme-labeled method was used for detecting ATP synthesis.Results:Cardiac structure and functions:Compared with the MI group at 1 week,the YQHX prescription group and the perindopril group exhibited increased LVEF and LVFS (Pall <.05);their LVEDs and LVEDd were reduced but suggested no statistically significant differences (F =2.258,F =0.3464,Pall >.05).At 4 weeks,both LVEF and LVFS were elevated in the YQHX prescription group (P =.008,.009) and the perindopril group (P =.279,.333),where differences in the later group indicated no statistical significance;the YQHX prescription group and the perindopril group were also featured by reduced LVEDs and LVEDd (Pall <.05).Morphology:Compared with the MI group at two time nodes,the YQHX prescription group and the perindopril group exhibited significant improvement in the pathological changes in myocardial cells and mitochondrial structure.Expression of AMPK signal pathway-related proteins and mRNA:The expression of both pLKB1 and pAMPK proteins followed a rising trend in the YQHX prescription group and the perindopril group at 1 week.The expression of LKB1mRNA and AMPKmRNA was elevated (Pall <.05).The increased expression of PGC-1α,NRF1 and mtTFA proteins demonstrated statistically significant differences (Pall <.05).The expression of mtDNA protein followed an increasing trend.At 4 weeks,the expression of both pLKB1 and pAMPK proteins was elevated (Pall <.05).Heightened expression was also reported in LKB1mRNA and AMPKmR-NA (Pall <.05).The increased expression of PGC-1α,NRF1,mtTFA and mtDNA proteins demonstrated statistically significant differences (Pall <.05).ATP synthesis:ATP synthesis was increased in the YQHX prescription group and the perindopril group at both 1 week and 4 weeks (Pall <.001).Conclusion:The possible mechanism of YQHX prescription for preventing and treating post-MI myocardial remodeling may function through strengthening the activation AMPK signal pathway by LKB1,thus further increasing the expression of downstream transcription factor proteins and initiating mitochondrial replication and transcription;as a result,the YQHX prescription can improve the post-MI damage in mitochondrial morphological structure in the tissue at heart marginal zone as well as enhance mitochondrial biosynthesis and ATP synthesis.