Insufficient metabolic energy,in the form of adenosine triphosphate(ATP),and bacterial infections are among the main causes for the development of chronic wounds.Previously we showed that the physi-ological inorganic ...Insufficient metabolic energy,in the form of adenosine triphosphate(ATP),and bacterial infections are among the main causes for the development of chronic wounds.Previously we showed that the physi-ological inorganic polymer polyphosphate(polyP)massively accelerates wound healing both in animals(diabetic mice)and,when incorporated into mats,in patients with chronic wounds.Here,we focused on a hydrogel-based gel formulation,supplemented with both soluble sodium polyP(Na-polyP)and amor-phous calcium polyP nanoparticles(Ca-polyP-NP).Exposure of human epidermal keratinocytes to the gel caused a significant increase in extracellular ATP level,an effect that was even enhanced when Na-polyP was combined with Ca-polyP-NP.Furthermore,it is shown that the added polyP in the gel is converted into a coacervate,leading to encapsulation and killing of bacteria.The data on human chronic wounds showed that the administration of hydrogel leads to the complete closure of these wounds.Histological analysis of biopsies showed an increased granulation of the wounds and an enhanced microvessel forma-tion.The results indicate that the polyP hydrogel,due to its properties to entrap bacteria and generate metabolic energy,is a very promising formulation for a new therapy for chronic wounds.展开更多
This paper investigated the effects of five kinds of Au surfaces terminated with and without functional groups on the crystallization of ferric oxides/oxyhydroxides in the suspension condition. Self-assembled monolaye...This paper investigated the effects of five kinds of Au surfaces terminated with and without functional groups on the crystallization of ferric oxides/oxyhydroxides in the suspension condition. Self-assembled monolayers (SAMs) were used to create hydroxyl (-OH), carboxyl (-COOH), amine (-NH2) and methyl (-CH3) functionalized surfaces, which proved to be of the same surface density. The immersion time of substrates in the Fe(OH)3 suspension was divided into two time portions. During the first period of 2 h, few ferric oxide/oxyhydroxide was deposited except that E-Fe203 was detected on -NH2 surface. Crystallization for 10h evidenced more kinds of iron compounds on the functional surfaces. Goethite and maghemite were noticed on four functional surfaces, and maghemite also grew on Au surface. Deposition of ^-Fe203 was found on -OH surface, while the growth of orthorhombic and hexagon FeOOH were indicated on -NH2 surface. Considering the wide existence of iron compounds in nature, our investigation is a precedent work to the study of iron biomineralization in the suspension area.展开更多
基金We are very much grateful to Dr.Beate Weidenthaler-Barth(De-partment of Dermatology,University Clinic Mainz)for the very expert histological analyses and the permission to include the images in this study.Moreover,we thank Mrs.Kerstin Bahr,Institute of Functional and Clinical Anatomy,University Medical Center,Mainz(Germany)for her continuous support.In addition,we are thankful to Mrs.Franziska S.Kranz(Medical Center of the Jo-hannes Gutenberg University,Mainz)for her important support.W.E.G.Müller is the holder of an ERC Advanced Investigator Grant(Grant No.268476)In addition,W.E.G.Müller has obtained three ERC-PoC grants(Si-Bone-PoC,Grant No.324564,MorphoVES-PoC,Grant No.662486,and ArthroDUR,Grant No.767234)+3 种基金In addition,this work was supported by grants from the European Commission(Grant Nos.604036 and 311848)the International Human Frontier Science Program,and the BiomaTiCS research initiative of the University Medical Center,Mainz.Further support came from the BMBF(Grant No.13GW0403A/B-SKIN-ENERGY)the BMWi(Grant No.ZF4294002AP9)the China National Key R&D Plan:China-German Cooperation(Grant No.2018YFE0194300).
文摘Insufficient metabolic energy,in the form of adenosine triphosphate(ATP),and bacterial infections are among the main causes for the development of chronic wounds.Previously we showed that the physi-ological inorganic polymer polyphosphate(polyP)massively accelerates wound healing both in animals(diabetic mice)and,when incorporated into mats,in patients with chronic wounds.Here,we focused on a hydrogel-based gel formulation,supplemented with both soluble sodium polyP(Na-polyP)and amor-phous calcium polyP nanoparticles(Ca-polyP-NP).Exposure of human epidermal keratinocytes to the gel caused a significant increase in extracellular ATP level,an effect that was even enhanced when Na-polyP was combined with Ca-polyP-NP.Furthermore,it is shown that the added polyP in the gel is converted into a coacervate,leading to encapsulation and killing of bacteria.The data on human chronic wounds showed that the administration of hydrogel leads to the complete closure of these wounds.Histological analysis of biopsies showed an increased granulation of the wounds and an enhanced microvessel forma-tion.The results indicate that the polyP hydrogel,due to its properties to entrap bacteria and generate metabolic energy,is a very promising formulation for a new therapy for chronic wounds.
文摘This paper investigated the effects of five kinds of Au surfaces terminated with and without functional groups on the crystallization of ferric oxides/oxyhydroxides in the suspension condition. Self-assembled monolayers (SAMs) were used to create hydroxyl (-OH), carboxyl (-COOH), amine (-NH2) and methyl (-CH3) functionalized surfaces, which proved to be of the same surface density. The immersion time of substrates in the Fe(OH)3 suspension was divided into two time portions. During the first period of 2 h, few ferric oxide/oxyhydroxide was deposited except that E-Fe203 was detected on -NH2 surface. Crystallization for 10h evidenced more kinds of iron compounds on the functional surfaces. Goethite and maghemite were noticed on four functional surfaces, and maghemite also grew on Au surface. Deposition of ^-Fe203 was found on -OH surface, while the growth of orthorhombic and hexagon FeOOH were indicated on -NH2 surface. Considering the wide existence of iron compounds in nature, our investigation is a precedent work to the study of iron biomineralization in the suspension area.