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风成沉积物红外及绿光释光测年比较研究 被引量:6
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作者 赖忠平 周杰 +1 位作者 卢演俦 SinghviA K 《海洋地质与第四纪地质》 CAS CSCD 北大核心 2000年第1期57-61,共5页
中国沙漠黄土边界带是地质时期东亚季风气候变化的敏感地带 ,其风成沉积物记录了东亚季风气候的变迁。对处于沙漠黄土边界带的吉林省太平川剖面进行了钾长石红外释光及石英绿光释光测年的比较研究 ,详细阐述了粗颗粒技术样品的前处理方... 中国沙漠黄土边界带是地质时期东亚季风气候变化的敏感地带 ,其风成沉积物记录了东亚季风气候的变迁。对处于沙漠黄土边界带的吉林省太平川剖面进行了钾长石红外释光及石英绿光释光测年的比较研究 ,详细阐述了粗颗粒技术样品的前处理方法及钾长石和石英单矿物的分离过程 ,对于预热而导致的热转移信号对光释光测年中等效剂量的影响作了探讨 。 展开更多
关键词 风成沉积 光释光测年 粗颗粒技术 热转移校正
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水平井开采南海神狐海域天然气水合物数值模拟 被引量:19
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作者 李刚 李小森 +1 位作者 KeniZhang GeorgeJ.Moridis 《地球物理学报》 SCIE EI CAS CSCD 北大核心 2011年第9期2325-2337,共13页
2007年5月,南海北部神狐海域的实地钻探结果表明该区域海底存在大量天然气水合物,其作为未来我国潜在的可开发能源的调查和资源评价工作正在展开.本文以SH7站位的钻探、测井数据为基础,建立了实际水合物藏分层地质模型,主要包括上盖层... 2007年5月,南海北部神狐海域的实地钻探结果表明该区域海底存在大量天然气水合物,其作为未来我国潜在的可开发能源的调查和资源评价工作正在展开.本文以SH7站位的钻探、测井数据为基础,建立了实际水合物藏分层地质模型,主要包括上盖层、水合物层和下盖层,其中上下盖层均为可渗透的沉积物.本文利用新型的开采井设计方式,进行了单一水平井定压降压法开采水合物的数值模拟.结果表明,开采过程中水合物分解区域主要集中在开采井周边区域、水合物层与上下盖层的界面附近区域.开采井产气量远小于存在不可渗透盖层的水合物藏,利用单一降压法不能经济有效地开采该区域天然气水合物. 展开更多
关键词 天然气水合物 降压开采 水平井 南海神狐海域
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裂隙中滞水区对溶质运移影响的模拟分析 被引量:4
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作者 李国敏 黎明 +1 位作者 韩巍 TSANG Chin-Fu 《岩石力学与工程学报》 EI CAS CSCD 北大核心 2007年第S2期3855-3860,共6页
无论是野外还是室内试验所涉及到的均是变隙宽的粗糙裂隙。重点探讨带滞水区的裂隙形态对其中溶质式示踪剂运移的影响及导致的运移特征变化。采用的模拟方法是考虑对流与分子扩散步骤的随机粒子追踪方法。数值模型区域选择均质导水介质... 无论是野外还是室内试验所涉及到的均是变隙宽的粗糙裂隙。重点探讨带滞水区的裂隙形态对其中溶质式示踪剂运移的影响及导致的运移特征变化。采用的模拟方法是考虑对流与分子扩散步骤的随机粒子追踪方法。数值模型区域选择均质导水介质。不同程度的分子扩散模式用于比较其对溶质运移结果——穿透曲线特征的影响。尝试从不同穿透曲线的特征分析来解析带滞水区裂隙的几何形态及相对分子扩散程度对溶质运移的影响。研究结果表明,在不带滞水区的单裂隙中,溶质几乎同时流出系统。对于相同分子扩散尺度下的带较宽或较深滞水区的裂隙,其对应的穿透曲线的高峰值明显降低并伴随着明显的"拖尾"现象。模拟结果还表明在较高尺度分子扩散条件下,其穿透曲线具有相类似的特征。 展开更多
关键词 数值分析 裂隙 滞水区 溶质运移 非均质介质
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Oda裂隙张量的DC模型在裂隙岩体渗流模拟中的应用 被引量:5
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作者 王臻 Jonny Rutqvist 戴瑛 《力学季刊》 CSCD 北大核心 2011年第4期486-492,共7页
针对裂隙岩体渗流非均质各向异性的特点,我们用基于离散连续模型(discrete continuum model,简称DC模型)的方法,对裂隙岩体渗流和溶质运移进行了数值模拟。在此工作中我们采用Oda裂隙张量理论计算各个单元的渗透张量,组成整个渗流区域... 针对裂隙岩体渗流非均质各向异性的特点,我们用基于离散连续模型(discrete continuum model,简称DC模型)的方法,对裂隙岩体渗流和溶质运移进行了数值模拟。在此工作中我们采用Oda裂隙张量理论计算各个单元的渗透张量,组成整个渗流区域的渗透张量场;并采用具有"旋转不变性"特征的九点差分格式,来减少网格离散方式对数值模拟结果的影响;并对一benchmark test model(BTM)模型进行了数值模拟。模拟结果与BTM结果很吻合。 展开更多
关键词 裂隙岩体渗流 离散连续模型 裂隙张量理论 九点差分格式
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深部CO_2泄漏对浅层地下水水质影响研究进展
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作者 杜尚海 苏小四 郑连阁 《水科学进展》 EI CAS CSCD 北大核心 2012年第6期875-880,共6页
由于盖层中存在的未知断层、裂隙或被废弃井穿透等原因,深部储存的CO2可能会发生渗漏,并向上迁移进入浅部含水层,改变地下水中酸度和溶解组分的浓度分布。国外开展CO2泄漏对浅层地下水水质影响相关研究尚处于起步阶段,且室内实验、野外... 由于盖层中存在的未知断层、裂隙或被废弃井穿透等原因,深部储存的CO2可能会发生渗漏,并向上迁移进入浅部含水层,改变地下水中酸度和溶解组分的浓度分布。国外开展CO2泄漏对浅层地下水水质影响相关研究尚处于起步阶段,且室内实验、野外试验和数值模拟等研究结果表明,CO2泄漏对浅层地下水中pH和微量重金属组分浓度影响显著,虽浓度大多未超饮用水标准,但由于含水介质之间矿物组成的差异较大,有必要针对具体场地的地下水水质和矿物组分特征进行调查,研究CO2侵入对地下水水质的影响,在总结已经达成共识和存在的问题基础上提出下一步研究趋势。 展开更多
关键词 二氧化碳 地质储存 泄漏 地下水水质 重金属污染
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岩石破裂以及破裂前信号的演变 被引量:4
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作者 Freund F T Dahlgren R P +2 位作者 Chu J j 张祯(译) 田家勇(校) 《国际地震动态》 2011年第1期110-114,共5页
地震是大量的岩石破裂事件。很多大地震之前都存在瞬态信号:电磁信号、磁场变化、电离层扰动、土壤导电率的变化以及其他震前信号。令监测人员奇怪的是,在震前短时间内一些震前信号会衰弱甚至消失不见。岩石中应力活化电流的发现提供了... 地震是大量的岩石破裂事件。很多大地震之前都存在瞬态信号:电磁信号、磁场变化、电离层扰动、土壤导电率的变化以及其他震前信号。令监测人员奇怪的是,在震前短时间内一些震前信号会衰弱甚至消失不见。岩石中应力活化电流的发现提供了一个可能的解释,应力能够激活岩石中的移动电荷载体,即电子空位缺陷(electron va-cancy defects,EVD),也称空穴,用h.表示。h.相当于O2-中的O-,且以电钝性及休止的状态存在于无应力岩石中。当被激活时,h.沿着应力梯度方向流动,从而产生电流。在非常高的应力速率下,位错累积成微破裂,这正是灾变破裂的开始。当h.电荷载体无法再被激活时,震前破裂信号会明显衰弱。 展开更多
关键词 岩石破裂 电磁信号 应力速率 演变 电荷载体 电离层扰动 瞬态信号 磁场变化
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地下非均匀非饱和带中地下洞室的渗流问题数值模拟——介质参数的灵敏度分析 被引量:8
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作者 李国敏 Chin Fu Tsang 《地球科学(中国地质大学学报)》 EI CAS CSCD 北大核心 2003年第5期497-504,共8页
影响任何一个地下废物处置场所长期行为的一个重要因素是进入地下废物贮置洞室内的渗流量的大小.预测地下洞室中的渗流量是困难的,特别是当地下废物贮置洞室位于非均质非饱和带中.三维数值模型用于研究地下非均质中非饱和流动及流入地... 影响任何一个地下废物处置场所长期行为的一个重要因素是进入地下废物贮置洞室内的渗流量的大小.预测地下洞室中的渗流量是困难的,特别是当地下废物贮置洞室位于非均质非饱和带中.三维数值模型用于研究地下非均质中非饱和流动及流入地下洞室中的渗流量.讨论了非均质模型与均质模型的比较以及数值剖分尺度对计算结果的影响.进入洞室中的渗漏率随着引入系统中入渗量的增加而增大.选用4个参数来衡量渗流场的非均质程度:(1)介质的平均渗透率K0;(2)VanGenuchten参数α;(3)渗透率空间分布相关尺度;(4)渗透率空间分布变化的标准差σ.根据一个随机实现的渗透率分布,通过改变平均渗透率来研究其对流入洞室中渗透量的影响.对一个固定的入渗率而言,流入洞室中的渗漏率将随着VanGenuchten参数α的减小而减小.模拟结果表明流入洞室的渗漏率与介质的平均渗透率相关,即随平均渗透率的增大,流入洞室的渗漏率亦增大.流入洞室的渗漏率还高度依赖于非均质渗透率场的空间分布相关长度与标准差.一个大的相关长度或高的标准差均能导致流入洞室的渗漏率增大. 展开更多
关键词 地下洞室 非均质 非饱和带 渗漏率 灵敏度分析 废物处置
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Carbon Turnover in a Crop Rotation Under Free Air CO_2 Enrichment (FACE) 被引量:12
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作者 H. J. WEIGEL A. PACHOLSKI +8 位作者 S. BURKART M. HELAL O. HEINEMEYER B. KLEIKAMP R. MANDERSCHEID C. FRüHAUF G. F. HENDREY K. LEWIN J. NAGY 《Pedosphere》 SCIE CAS CSCD 2005年第6期728-738,共11页
. Mostly based on assumptions derived from controlled-environment studies, predicted future atmospheric CO2 concen- trations [CO2] are expected to have considerable impacts on carbon (C) turnover in agro-ecosystems. I... . Mostly based on assumptions derived from controlled-environment studies, predicted future atmospheric CO2 concen- trations [CO2] are expected to have considerable impacts on carbon (C) turnover in agro-ecosystems. In order to allow the in situ examination of C-transformations in the plant-soil system of arable crop rotations under future [CO2], a free air carbon dioxide enrichment (FACE) experiment (550 μmol mol-1 CO2) was started at Braunschweig, Germany in 1999. The crop rotation under investigation comprised winter barley, a cover crop (ryegrass), sugar beets and winter wheat. Assessments of CO2 effects included the determination of above- and belowground biomass production, measurements of canopy CO2- and H2O- fluxes, soil microbial biomass and in situ soil respiration. The results obtained during the 1st crop rotation cycle (3 years) showed that for the selected crops elevated [CO2] entailed significant positive effects (P < 0.05) on aboveground (6%-14% stimulation) and belowground biomass production (up to 90% stimulation), while canopy evapotranspiration was reduced. This resulted in increased soil water content. Also, depending on crop type and season, high CO2 stimulated in situ soil respiration (up to 30%), while soil microbial biomass did not show significant responses to elevated [CO2] during the first rotation cycle. 展开更多
关键词 农业生态系统 气候变化 二氧化碳 土壤 生物循环
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Wheat, maize and sunflower cropping systems selectively influence bacteria community structure and diversity in their and succeeding crop's rhizosphere 被引量:19
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作者 WEN Xin-ya Eric Dubinsky +2 位作者 WU Yao YU Rong CHEN Fu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第8期1892-1902,共11页
supported by the Special Fund for Agro-scientific Research in the Public Interest in China (201103001)
关键词 bacterial community structure and diversity rhizosphere cropping system 454 pyrosequencing
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The Global Stratotype Section and Point (GSSP) for the base of the Katian Stage of the Upper Ordovician Series at Black Knob Ridge, Southeastern Oklahoma, USA 被引量:9
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作者 Daniel Goldman Stephen A. Leslie +3 位作者 Jaak Nōlvak Seth Young Stig M. Bergstroem Warren D. Huff 《Episodes》 SCIE 2007年第4期258-270,共13页
关键词 层形断面 奥陶纪 俄克拉荷马州 美国 地质特点
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Light-absorbing Particles in Snow and Ice: Measurement and Modeling of Climatic and Hydrological impact 被引量:19
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作者 Yun QIAN Teppei J.YASUNARI +7 位作者 Sarah J.DOHERTY Mark G.FLANNER William K.M.LAU MING Jing Hailong WANG Mo WANG Stephen G.WARREN Rudong ZHANG 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2015年第1期64-91,共28页
Light absorbing particles(LAP, e.g., black carbon, brown carbon, and dust) influence water and energy budgets of the atmosphere and snowpack in multiple ways. In addition to their effects associated with atmospheric... Light absorbing particles(LAP, e.g., black carbon, brown carbon, and dust) influence water and energy budgets of the atmosphere and snowpack in multiple ways. In addition to their effects associated with atmospheric heating by absorption of solar radiation and interactions with clouds, LAP in snow on land and ice can reduce the surface reflectance(a.k.a., surface darkening), which is likely to accelerate the snow aging process and further reduces snow albedo and increases the speed of snowpack melt. LAP in snow and ice(LAPSI) has been identified as one of major forcings affecting climate change, e.g.in the fourth and fifth assessment reports of IPCC. However, the uncertainty level in quantifying this effect remains very high. In this review paper, we document various technical methods of measuring LAPSI and review the progress made in measuring the LAPSI in Arctic, Tibetan Plateau and other mid-latitude regions. We also report the progress in modeling the mass concentrations, albedo reduction, radiative forcing, and climatic and hydrological impact of LAPSI at global and regional scales. Finally we identify some research needs for reducing the uncertainties in the impact of LAPSI on global and regional climate and the hydrological cycle. 展开更多
关键词 light-absorbing aerosol snow ice albedo measurement climate modeling hydrological cycle
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Implications of a new, biostratigraphically well-controlled, radio-isotopic age for the lower Telychian Stage of the Llandovery Series (Lower Silurian, Sweden) 被引量:2
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作者 Stig M. Bergstrom Funda Ozlem Toprak +1 位作者 Warren D. Huff Roland Mundil 《Episodes》 SCIE 2008年第3期309-314,共6页
关键词 无线电 同位素 蓝达夫里阶 志留纪
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应力激活电流与软化岩石
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作者 Freund F T Hoenig S A +5 位作者 Braun A Dahlgren R P Momayez M Chu J J 米琦(译) 张琴琴(校) 《国际地震动态》 2011年第1期104-109,共6页
当岩石受到机械应力作用时,隐藏的电子缺陷就被激活。这种激活会产生通过释放高移动性的缺陷电子使岩石电导率增加的电子空穴对,相当于O2-点阵中存在的O-,叫做正空穴,用符号h.表示。这种电荷载体h.能够从受到应力作用的岩石中扩散到周... 当岩石受到机械应力作用时,隐藏的电子缺陷就被激活。这种激活会产生通过释放高移动性的缺陷电子使岩石电导率增加的电子空穴对,相当于O2-点阵中存在的O-,叫做正空穴,用符号h.表示。这种电荷载体h.能够从受到应力作用的岩石中扩散到周围未受应力作用的岩石中。阻止h.流出改变了岩石的力学属性:使它们得到软化和弱化。进行中的研究针对与电荷载体h.相关的波函数的非定域作用,这种作用广泛影响到许多周围的O2-。尽管这种正空穴的数量密度可能低至千分之一,但实质上岩石子集中所有的O2-失去了一些电子密度。这种电子缺失会弱化阴阳离子间原子键的作用,从而影响到岩石的力学性质。 展开更多
关键词 应力作用 激活电流 岩石 软化 电子空穴对 电荷载体 电子密度 力学性质
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Geodetic Datum Transformation Parameters towards WGS84 Applicable to the 1/50k Topographical Map (1981) of Burundi 被引量:1
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作者 Johan Lavreau 《International Journal of Geosciences》 2016年第5期631-636,共6页
The knowledge of the geodetic reference datum of maps or data is required for their use in a GIS. Many older maps are lacking this information, making their use cumbersome. The availability of an aerial coverage at hi... The knowledge of the geodetic reference datum of maps or data is required for their use in a GIS. Many older maps are lacking this information, making their use cumbersome. The availability of an aerial coverage at high resolution of Burundi and digital elevation model, based on a novel geodetic network, all calculated on the WGS84 datum, allows to calculate the datum applicable to older maps, for instance the regular 1/50k map of the country. The method, based on the difference in geocentric coordinates between points common to the two systems, yields: Δx = -156.71 ± 10.2 m, .Δy = -3.26 ± 13.2 m, .Δz = -290.77 ± 21.06 m, well in keeping with older values proposed by the NGA. 展开更多
关键词 GEODESY CARTOGRAPHY Datum Calculation BURUNDI
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Effects of Climate and the Urban Heat Island Effect on Urban Tree Growth in Houston 被引量:1
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作者 Astrid Moser Enno Uhl +4 位作者 Thomas Rotzer Peter Biber Jens Dahlhausen Barry Lefer Hans Pretzsch 《Open Journal of Forestry》 2017年第4期428-445,共18页
The growing conditions of urban trees differ substantially from forest sites and are mainly characterized by small planting pits with less water, nutrient and aeration availability, high temperatures and radiation inp... The growing conditions of urban trees differ substantially from forest sites and are mainly characterized by small planting pits with less water, nutrient and aeration availability, high temperatures and radiation inputs as well as pollution and soil compaction. Especially, global warming can amplify the negative effects of urban microclimates on tree growth, health and well-being of citizens. To quantify the growth of urban trees influenced by the urban climate, ten urban tree species in four climate zones were assessed in an overarching worldwide dendrochronological study. The focus of this analysis was the species water oak (Quercus nigra L.) in Houston, Texas, USA. Similar to the overall growth trend, we found in urban trees, water oaks displayed an accelerated growth during the last decades. Moreover, water oaks in the city center grew better than the water oaks growing in the rural surroundings of Houston, though this trend was reversed with high age. Growth habitat (urban, suburban, rural and forest) significantly affected tree growth (p < 0.001) with urban trees growing faster than rural growing trees and forest trees, though a younger age of urban trees might influence the found growth patterns. Growing site in terms of cardinal direction did not markedly influence tree growth, which was more influenced by the prevalent climatic conditions of Houston and the urban climate. Higher temperatures, an extended growing season and eutrophication can cause an accelerated growth of trees in urban regions across, across all climatic zones. However, an accelerated growth rate can have negative consequences like quicker ageing and tree death resulting in higher costs for new plantings and tree management as well as the decrease in ecosystem services due to a lack of old trees providing greatest benefits for mitigating the negative effects of the urban climate. 展开更多
关键词 Climate Change Growth Trends Heat Island Effect Quercus nigra Tree Ring Analyses
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利用二氧化碳作为工作流的增强型地热系统(EGS)——用于再生能源和碳储存的新方法 被引量:1
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作者 Karsten Pruess 葛秀珍(翻译) 何雪洲(校对) 《水文地质工程地质技术方法动态》 2011年第3期56-66,共11页
为应对二氧化碳减排需求,布朗2000年提出了新的增强型地热系统(EGS)的概念,即利用二氧化碳替代水作为热传导流。这样不但达到了二氧化碳地质储存的目的而且还带来了附加效益。根据他的建议,我们评价了热物理学性质,进行丁数字模... 为应对二氧化碳减排需求,布朗2000年提出了新的增强型地热系统(EGS)的概念,即利用二氧化碳替代水作为热传导流。这样不但达到了二氧化碳地质储存的目的而且还带来了附加效益。根据他的建议,我们评价了热物理学性质,进行丁数字模拟,针对用二氰化碳作为工作流设计的热储层,我们探讨了流体动力学和热传输问题。我们发现,开采热碎岩的热量,二氧化碳比水的作用要大一些。就井液压而言,二氧化碳还可以提供某些优势,与水相比,由于它的压缩性和膨胀性,二氧化碳将增加浮力,降低冲洗液循环系统附加的能量消耗。CO2-EGS系统存热与液压方面是有潜力的,较大的不确定性主要是水流与岩石之间相互化学作用。用二氧化碳作为注入流体的EGS系统对于进一步调查研究极具吸引力。 展开更多
关键词 增强型地热系统 二氧化碳 数值建模
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The effects of carbon dioxide leakage on fractures, and water quality of potable aquifers during geological sequestration of CO_2
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作者 Wei ZHANG Yilian LI +2 位作者 Tianfu XU Wei QIANG Shangping XIAO 《Chinese Journal Of Geochemistry》 EI CAS 2006年第B08期58-58,共1页
关键词 二氧化碳 数值模拟 含水层 重金属 孔隙度 水质
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Dynamic Responses of Atmospheric Carbon Dioxide Concentration to Global Temperature Changes between 1850 and 2010
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作者 Weile WANG Ramakrishna NEMANI 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2016年第2期247-258,共12页
Changes in Earth's temperature have significant impacts on the global carbon cycle that vary at different time scales, yet to quantify such impacts with a simple scheme is traditionally deemed difficult. Here, we sho... Changes in Earth's temperature have significant impacts on the global carbon cycle that vary at different time scales, yet to quantify such impacts with a simple scheme is traditionally deemed difficult. Here, we show that, by incorporating a tem- perature sensitivity parameter (1.64 ppm yr-1 ℃-1) into a simple linear carbon-cycle model, we can accurately characterize the dynamic responses of atmospheric carbon dioxide (CO2) concentration to anthropogenic carbon emissions and global temperature changes between 1850 and 2010 (r2 〉 0.96 and the root-mean-square error 〈 1 ppm for the period from 1960 onward). Analytical analysis also indicates that the multiplication of the parameter with the response time of the atmospheric carbon reservoir (-12 year) approximates the long-term temperature sensitivity of global atmospheric CO2 concentration (-15 ppm ℃ 1), generally consistent with previous estimates based on reconstructed CO2 and climate records over the Little Ice Age. Our results suggest that recent increases in global surface temperatures, which accelerate the release of carbon from the surface reservoirs into the atmosphere, have partially offset surface carbon uptakes enhanced by the elevated atmo- spheric CO2 concentration and slowed the net rate of atmospheric CO2 sequestration by global land and oceans by -30% since the 1960s. The linear modeling framework outlined in this paper thus provides a useful tool to diagnose the observed atmospheric CO2 dynamics and monitor their future changes. 展开更多
关键词 atmospheric CO2 dynamics climate-carbon interactions climate change carbon cycle
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Modeling of the Water Table Level Response Due to Extraordinary Precipitation Events: The Case of the Guadalupe Valley Aquifer
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作者 Javier González Ramírez Rogelio Vázquez González 《International Journal of Geosciences》 2013年第6期950-958,共9页
A two-dimensional algorithm for underground water flow simulation was modified and adapted to the geohydrologic conditions of the Guadalupe valley located in the state of Baja California in northern México. In or... A two-dimensional algorithm for underground water flow simulation was modified and adapted to the geohydrologic conditions of the Guadalupe valley located in the state of Baja California in northern México. In order to solve the numerical model using the balance equation, the central finite differences with spatial and temporal constant increments method were used. Such model considers a heterogeneous and transient unconfined aquifer. Modeling and calibration processes are presented using the data of water table levels provided by the water level data loggers installed in a monitoring network and precipitation data from climatic stations of both seasons: 2009-2010 and 2010-2011. It was possible to locate, correlate and model specific rainfall-recharge events with the aim of obtaining an estimation of how these events are directly reflected on the water table level of the aquifer and how it reacts against simulated extraordinary events. 展开更多
关键词 Geohydrologic Model PRECIPITATION RECHARGE FINITE DIFFERENCES Method
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A<i>β</i>-Like Peptide Displayed on Bacteriophage T7 Catalyzes Chromate and Uranyl Reduction
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作者 Hongjun Jin Chiann-Tso Lin +6 位作者 Jianying Shang Michael J. Wilkins Yingbin Liu Wei Gong Weiping Xu Thomas C. Squier Philip E. Long 《Journal of Environmental Protection》 2013年第8期857-868,共12页
In order to discover genes capable of catalyzing the reductive immobilization of toxic chromate and uranyl ions, we have created a T7 bacteriophage library containing cDNA from environmental microbes (i.e., Geobacter ... In order to discover genes capable of catalyzing the reductive immobilization of toxic chromate and uranyl ions, we have created a T7 bacteriophage library containing cDNA from environmental microbes (i.e., Geobacter sulfurreducens and Shewanella oneidensis MR-1) that are known to mediate the reduction of chromate and uranyl ions. After three rounds of screening, ten bacteriophage mutants were found to mediate the NADH-dependent reduction of chromate and uranyl ions whose cDNA encodes polypeptide chains ranging 14 to 73 amino acids in length. All identified sequences contain disordered structural motifs similar to the β-amyloid peptide (Aβ) known to promote aggregation and formation of high-affinity metal binding sites. Confirmation of this structural similarity involved phage display of the 42 amino-acid Aβ-peptides that have been found to catalyze the NADH-dependent reduction of both chromate and uranyl ions. Transmission electron microscopy (TEM) and X-ray absorption near edge structure (XANES) measurements confirm that reduced uranium is present on the surface of bacteriophage expressing the Aβ-peptide. The surface-displayed Aβ-like peptide on bacteriophage has the potential to couple naturally occurring electron transfer shuttles present in soils to promote economically viable remediation of contaminated sites containing toxic chromate and uranyl ions. 展开更多
关键词 CHROMATE URANYL BIOREMEDIATION BACTERIOPHAGE
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