Distribution and diversity of microbial communities in meromictic soda Lake Doroninskoe(Transbaikalia,Russia) during winter

Meromictic soda and saline lakes are unique ecosystems characterized by the stability of physical,chemical and biological parameters,and they are distributed all over the world.Lakes located in regions with average annual negative air temperature are of particular interest because of the presence of two periods with intensive and dynamic processes:the so-called biological summer and the long ice season with the biological spring.Soda Lake Doroninskoe is located in Eastern Transbaikalia(51°14′N,112°14′E) in the permafrost zone in an extreme continental climate,and is covered by ice for seven months per year.The structure and diversity of the microbial communities throughout the water column of the lake was studied by 16 S r RNA gene amplicon metasequencing.Different species with specific functions were found to dominate at different depths.Metabolically flexible bacteria with a capacity to switch between anoxygenic photosynthesis and aerobic chemotrophic metabolism dominate in soda Lake Doroninskoe.

Structure and diversity dynamics of microbial communities at day and night:investigation of meromictic Lake Doroninskoe,Transbaikalia,Russia

Extreme environmental conditions are key factors in the formation of the structure and diversity of microbial communities. In meromictic ecosystems, extreme conditions and a stable stratification of physical, chemical and biological parameters lead to diversity and heterogeneity of microenvironments. Lake Doroninskoe is located in an extreme geographical area and diff ers from other known meromictic reservoirs of the world by a low level of illumination in the chemocline and a rare type of alkaline water in sedimentary rocks formed by evaporative concentration. To understand the key factors that shape the composition and structure of the microbial community, the macro-and micro-variations in space and time are of great importance. We investigated the short-term dynamics of the structure and diversity of microbial communities of the meromictic soda lake, Lake Doroninskoe, at day and night using highthroughput sequencing and bioinformatics. Metagenomic analysis of 16 S rRNA gene amplicons showed that the microbial communities had a high taxonomic diversity both at day and night. Sixteen bacterial and three archaeal phyla were identified. Proteobacteria were dominant and comprised 75% during the day, increasing to 90% at night. Metabolically stable denitrifying bacteria that were able to use a variety of alternative electron acceptors and electron donors were prevalent in Lake Doroninskoe. They belonged to the families Enterobacteriaceae(class Gammaproteobacteria) and Alcaligenaceae(class Betaproteobacteria). Statistically significant differences between day and night microbial communities were found. During the day, the microbial community was the most diverse. We discuss the peculiarities of the underexplored shortterm dynamics of the structure and diversity of the microbial communities of the meromictic soda Lake Doroninskoe, and propose topics for prospective studies.

The ecosystem of Lake Kenon:past and present(Transbaikal Territory,Russia)

Present-day conditions of the Lake Kenon ecosystem are determined by a combination of natural and anthropogenic factors.We have estimated the effects of a complex of factors on the condition of the abiotic environment and on specific biological components in the lake ecosystem.Change in biogenic load has caused an increase in the role of phytoplankton in the general balance of organic matter during the high-water period.Charophytes are the main dominants of bottom vegetation.Anthropogenic load has caused a decrease in both fish species and fish capacity.The lake application as a water reservoir-cooler has influenced the average annual water mineralization(from 420 mg/L to 530.0 mg/L with a maximum 654 mg/L in 1993) and fluctuations in its hydrochemical composition.The present composition of the lake is sulfate-hydrocarbonate-chloride calcium-sodic-magnesium in character.SO 4 – content is twice as much as the maximum permissible concentration in fishery waters.Water drainage from an ash disposal area to the lake has caused an increase in chemical-element concentrations including the heavy metals.Hg concentration in Perca fluviatilis muscles is 0.5 μg/g dry wt.Thus,understanding directions in the ecosystem of the water reservoir-cooler under changing hydrological conditions will let us forecast the consequences of new combined heat and power plant operation.

Morphology and biology of Cyclops scutifer Sars, 1863 in high mountain lakes of East Siberia (including Lake Amut)

Data on zooplankton from 13 high-mountain lakes of East Siberia have shown that the Holarctic copepod Cyclops scutifer Sars,1863 dominates among crustaceans.In July,its abundance comprised 64%-98%of the total plankton fauna in the pelagial of these lakes,approximately 30%in the littoral zone and10%in small northern thermokarst lakes.Biometric measurements and morphological descriptions based on scanning microscope images are supplemented by the data on its geographic distribution and phenology.

Chemical composition and sulfur forms in saline lakes of Kulunda Plain(Russia)

1 Introduction On the territory Kulunda Plain,located to the south-west of the Ob’plateau,there are more than 3,000 fresh and salt lakes with water TDS range from 1 to 430 g/L.The interest in these lakes was associated mainly with the study of

Palynological characteristics of Middle and Upper Jurassic deposits in East Transbaikalye

The author dwells on palynological characteristics of Middle and Upper Jurassic continental deposits in Transbaikalye within the Chita region adjacent to the territory of China. The paper contains descriptions of three sets of spores and pollen taken from Shadoronsk and one set from Undino-Dainsk biostratigraphic horizons. It also provides comparison characteristics of palynocomplexes that belong to Middle and Upper Jurassic deposits in East Transbaikalye with coeval complexes in Siberia and Kazakhstan and provides geological sequence of their formation.

横穿贝加尔湖山脉北部寒区的线性工程(英文)

The development of North Transbaikalye mineral resources (e.g. the Chiney deposit of iron titanium-vanadium-magnetite ores and Udokan copper deposits) is impossible without the establishment of industrial and transport infrastructure, one component of which are linear engineering constructions (drive railways, by-line auto roads, platform passages, protecting constructions, and others). However, on condition of spreading permafrost rocks this is conjugated with certain ecological risk just as from the transport network objects so for the objects themselves. A special attention is paid in the paper to considering of problems of projecting and constructing railways on the Udokan ridge.

寒区岩屑堆中汞化合物行为研究(英文)

At gold mining in the 19th～20th centuries on technogenous deposits of cryolitozone, considerable amounts of mercury-containing elements (amalgams) have been accumulated. Urgency of their utilization is caused by necessity of providing safe vital activity. It can be achieved when elaborating up-to-date technologies providing self-support of utilization as a consequence of extracting precious metals. Difficulty of this problem realization is caused by the absence of theoretical and applied elaborations of making the appropriate works in cryolitozone. The main trends of the problem solving on the grounds of creating scientific method foundations for investigating the cryolitozone amalgams transformation have been considered in the paper. Dynamics has been shown and the revealed regularities of their transformation, testifying of the occurrence of hydrometallurgic processes in cryolitozone massifs in situ, resulted in the decline of gold reserves at decreasing dispersity and increasing hallmarking, have been analyzed.

Phylogenetic Diversity of Microorganisms from Chemocline of the Meromictic Soda Lake Doroninskoe(Zabaikalie,Russia)

1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).This creates an

Lake-margin ecosystems of saline lakes of the Borzya group(Zabaikalsky Krai, Russia) during the initial filling phase

This article presents the results of hydrochemical and hydrobiological studies of shallow saline lakes of the Borzya group(Zabaikalsky Krai, Russia) at the initial filling phase. The lake-margin ecosystems of the studied lakes are characterized by varying degrees of salinity from polyhaline to brine water. Cyclical variations of meteorological conditions and high salinity determined that the compositions of the aquatic organisms are specific, mostly between stenohaline and euryhaline species, the quantities are low, and the community structures are simple.

Technogenic hydrogeochemical anomalies of tungsten deposits in Kykylbey ore region

Peculiarities of the tungsten deposits drainage flow chemical composition formation, the development of which was ceased almost 40 years ago, have been considered. Migration peculiarities of ore components have been covered, and forms of their migration have been calculated. Inertial characteristics of the surface flow contamination are shown.

外贝加尔大骨节病地区水文地球化学特征分析(英文)

本文通过对在外贝加尔大骨节病区水文地球化学研究结果表明,该地区天然水呈现出高浓度的磷(高达3.5mg/L),同时,锰、硅、铅等元素也呈现出高浓度的地球化学特征。分析水文地球化学数据,认为大骨节病可能与生物地球化学性质的多种因素有关系。进一步研究大骨节病的成因机制需要新的可靠的环境地球化学数据。

在寒区金矿开采过程中保护环境的土工措施研究(英文)

Causes and consequences of cryolitozone environmental contamination by technogenous mercury have been studied in the paper. Measures have been elaborated and methods for increasing ecological safety of the territories affected by mercury-containing mining wastes have been suggested. It has been proved that comprehensive utilization of mercury-containing heaps allows to solve problems of ecological safety with the least economical expenses.

Basic formation mechanisms of Lake Doroninskoye soda water, East Siberia, Russia

The primary scientific goal of studying salt lakes is to better understand the formation of small continentaltype hydrogeochemical systems. Many scientists have attributed the metamorphism of the chemical composition of salt lakes to the evaporative concentration of water. However, the formation of soda water is inconsistent with this hypothesis. Thus, analyzing intrabasinal biochemical processes and water—rocks interactions during the evaporative concentration of water allows us to understand the major mechanisms of the formation and evolution of water compositions. Therefore, the aim of this paper is to identify the key processes involved in the formation of the chemical composition of the water in Lake Doroninskoye. An analysis of the distribution of major components shows that Na^+,HCO^-_3, CO^(2-)_3, and Cl-are dominant in this water. High concentrations of these elements are the result of evaporative water concentration. Calcium, magnesium, and potassium are not accumulated because the water is saturated in minerals containing these elements. The main barrier to the growth of the sulfate content of water is sulfate reduction.This process also contributes to the additional reproduction of carbon dioxide, which reacts with the products of the hydrolysis of aluminosilicates OH-to form HCO^-_3 and CO^(2-)_3, thus further contributing to the natural processes of soda formation.

俄罗斯外贝加尔氟的水文地球化学异常(英文)

在本文中,5种类型的氟的水文地球化学异常被确定,它们的形成过程、在水环境中的流入物源以及浓度水平各异。对它们的特征也进行了讨论。

Vertical Distribution of Bacteria in Doroninskoe Lake(Zabaikalie,Russia):Paradigm of Dominance

1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial community and environmentalfactors offer useful information that bothleads to understanding of the process of element

All Season Observations of Meromictic Regime at Lake Doroninskoye

1 Introduction Soda Lake Doroninskoye(Uleoti District of Transbaikalian Region,Russia)belongs to a rare type of meromictic basins.For meromictic basins,the vertical stratification in the form of sufficiently thin water layers with significantly differing physical and chemical properties is typical,as well as peculiar biota.The main characteristics of the lake Doroninskoye are:

Biogeochemical conversion of sulfur species in saline lakes of Steppe Altai

The aim of the present research is to identify the main mechanisms of sulfur behavior in saline lakes in the course of time and followed transformations in their chemical composition. The influence of water on chemical composition of biochemical processes involved in decomposition of organic matter was determined by the study of behavior of reduced forms of sulfur in lakes. The determination of reduced forms of sulfur was carried out by successive transfer of each form of sulfur to hydrogen sulfide followed by photometric measurements. The other chemical components were determined by standard methods(atomic absorption, potentiometric method, titration method and others). The salt lakes of the Altai steppe were studied in summer season 2013–2015. Analysis of the chemical composition of the saline lakes of Altai Krai has shown that carbonate-, hydrocarbonate-and chloride ions dominate among anions; sodium is main cation; sulfates are found in subordinate amounts. Reduced forms of sulfur occur everywhere: hydrogen and hydrosulfide sulfur S^(2-) prevail in the bottom sediments; its derivative—elemental S^0—prevails in the lakes water. The second important species in water of soda lakes is hydrosulfide sulfur S^(2-), and in chloride lakes is thiosulfate sulfur S_2O_2^(3ˉ). The lag in the accumulation of sulfates in soda lakes in comparison to chloride lakes can be explained by their bacterial reduction, followed by the formation and deposition of iron sulfides in sediments. In chloride lakes gypsum is a predominantly barrier for sulfates.