AIM： To compare the clinical performance of 4 spectraldomain（SD） optical coherence tomography angiography（OCTA） systems： AngioVueTM, AngioPlexTM, Spectralis？ OCTA, AngioScan, and 1 swept-source（SS） OCTA SS OC...AIM： To compare the clinical performance of 4 spectraldomain（SD） optical coherence tomography angiography（OCTA） systems： AngioVueTM, AngioPlexTM, Spectralis？ OCTA, AngioScan, and 1 swept-source（SS） OCTA SS OCT AngioTM. METHODS： Twenty-seven undilated right eyes of 27 participants underwent OCTA examination using five different systems respectively for both 3×3 and 6×6 mm^2 scan pattern（Spectralis OCTA for 3×3 mm^2 scan only）. Image quality, including vessel valid visibility and the number of motion artifacts, and acquisition time were evaluated. Repeated measures analysis of variance（ANOVA） with Bonferroni＇s post-test and Friedman test with Dunn＇s posttest were used to compare measurements.RESULTS： The age of the subjects was 28.19±5.55 y（range, 23-49 y）. The spherical equivalent refraction was-2.55±1.84 D（range, 0.00 to-5.25 D）. Significant difference was observed in the evaluation of vessel valid visibility（Angio Vue the highest： 0.111±0.031 for 3×3 mm^2 scan and 0.128±0.020 for 6×6 mm^2 scan）, number of motion artifacts（AngioVue the fewest： 0.778±1.086 for 3×3 mm^2 scan and 0.333±0.620 for 6×6 mm^2 scan） and acquisition time（AngioPlex the shortest： 8.537±1.921 s for 3×3 mm^2 scan and 8.298±1.741 s for 6×6 mm^2 scan; all P〈0.001）. CONCLUSION： There is poor agreement of measurements among systems. AngioVue provides images with the highest vessel valid visibility and the fewest motion artifacts. AngioPlex achieves the shortest acquisition.展开更多
Colonoscopy is an effective tool for early screening of colorectal diseases.However,the application of colonoscopy in distinguishing different intestinal diseases still faces great challenges of efficiency and accurac...Colonoscopy is an effective tool for early screening of colorectal diseases.However,the application of colonoscopy in distinguishing different intestinal diseases still faces great challenges of efficiency and accuracy.Here we constructed and evaluated a deep convolution neural network(CNN)model based on 117055 images from 16004 individuals,which achieved a high accuracy of 0.933 in the validation dataset in identifying patients with polyp,colitis,colorectal cancer(CRC)from normal.The proposed approach was further validated onmulti-center real-time colonoscopy videos and images,which achieved accurate diagnostic performance on detecting colorectal diseases with high accuracy and precision to generalize across external validation datasets.The diagnostic performance of the model was further compared to the skilled endoscopists and the novices.In addition,our model has potential in diagnosis of adenomatous polyp and hyperplastic polyp with an area under the receiver operating characteristic curve of 0.975.Our proposed CNN models have potential in assisting clinicians in making clinical decisions with efficiency during application.展开更多
We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue sections.Immunohistochemistry(IHC)comprises a sequence of specific biochemical reactions and allows the detec...We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue sections.Immunohistochemistry(IHC)comprises a sequence of specific biochemical reactions and allows the detection of specific proteins in tissue sections.For the rapid implementation of IHC,we fabricated horizontally oriented microfluidic probes(MFPs)with functionally designed apertures to enable square and circular footprints,which we employ to locally expose a tissue to time-optimized sequences of different biochemicals.We show that the two main incubation steps of IHC protocols can be performed on MDAMB468-1510A cell block sections in less than 30 min,compared to incubation times of an hour or more in standard protocols.IHC analysis on the timescale of tens of minutes could potentially be applied during surgery,enabling clinicians to react in more dynamically and efficiently.Furthermore,this rapid IHC implementation along with conservative tissue usage has strong potential for the implementation of multiplexed assays,allowing the exploration of optimal assay conditions with a small amount of tissue to ensure high-quality staining results for the remainder of the sample.展开更多
基金Supported by National Key Research and Development Program for Precision Medicine of China (No.2016YFC0904800)
文摘AIM： To compare the clinical performance of 4 spectraldomain（SD） optical coherence tomography angiography（OCTA） systems： AngioVueTM, AngioPlexTM, Spectralis？ OCTA, AngioScan, and 1 swept-source（SS） OCTA SS OCT AngioTM. METHODS： Twenty-seven undilated right eyes of 27 participants underwent OCTA examination using five different systems respectively for both 3×3 and 6×6 mm^2 scan pattern（Spectralis OCTA for 3×3 mm^2 scan only）. Image quality, including vessel valid visibility and the number of motion artifacts, and acquisition time were evaluated. Repeated measures analysis of variance（ANOVA） with Bonferroni＇s post-test and Friedman test with Dunn＇s posttest were used to compare measurements.RESULTS： The age of the subjects was 28.19±5.55 y（range, 23-49 y）. The spherical equivalent refraction was-2.55±1.84 D（range, 0.00 to-5.25 D）. Significant difference was observed in the evaluation of vessel valid visibility（Angio Vue the highest： 0.111±0.031 for 3×3 mm^2 scan and 0.128±0.020 for 6×6 mm^2 scan）, number of motion artifacts（AngioVue the fewest： 0.778±1.086 for 3×3 mm^2 scan and 0.333±0.620 for 6×6 mm^2 scan） and acquisition time（AngioPlex the shortest： 8.537±1.921 s for 3×3 mm^2 scan and 8.298±1.741 s for 6×6 mm^2 scan; all P〈0.001）. CONCLUSION： There is poor agreement of measurements among systems. AngioVue provides images with the highest vessel valid visibility and the fewest motion artifacts. AngioPlex achieves the shortest acquisition.
基金This study was funded by the National Natural Science Foundation of China(Grant No.81902861 to F.Z.and 32000485 to X.H.)“Xinglin Scholars”Scientific Research Project Fund of Chengdu University of Traditional Chinese Medicine(Grant No.YYZX2019012 to F.Z.)+1 种基金the Scientific Research Fund of Technology Bureau in Dazhou(Grant No.17YYJC0004 to F.-W.Z.)the Key Research and Development Project Fund of Science and Technology Bureau in Dazhou,Sichuan Province(Grant No.20ZDYF0001 to F.-W.Z.).We express our deepest appreciation to J.Z,Y.C.for organizing the raw data and G.Y.for the revising the manuscript.
文摘Colonoscopy is an effective tool for early screening of colorectal diseases.However,the application of colonoscopy in distinguishing different intestinal diseases still faces great challenges of efficiency and accuracy.Here we constructed and evaluated a deep convolution neural network(CNN)model based on 117055 images from 16004 individuals,which achieved a high accuracy of 0.933 in the validation dataset in identifying patients with polyp,colitis,colorectal cancer(CRC)from normal.The proposed approach was further validated onmulti-center real-time colonoscopy videos and images,which achieved accurate diagnostic performance on detecting colorectal diseases with high accuracy and precision to generalize across external validation datasets.The diagnostic performance of the model was further compared to the skilled endoscopists and the novices.In addition,our model has potential in diagnosis of adenomatous polyp and hyperplastic polyp with an area under the receiver operating characteristic curve of 0.975.Our proposed CNN models have potential in assisting clinicians in making clinical decisions with efficiency during application.
基金This work was supported by the European Research Council PoC Grant CellProbe(842790).
文摘We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue sections.Immunohistochemistry(IHC)comprises a sequence of specific biochemical reactions and allows the detection of specific proteins in tissue sections.For the rapid implementation of IHC,we fabricated horizontally oriented microfluidic probes(MFPs)with functionally designed apertures to enable square and circular footprints,which we employ to locally expose a tissue to time-optimized sequences of different biochemicals.We show that the two main incubation steps of IHC protocols can be performed on MDAMB468-1510A cell block sections in less than 30 min,compared to incubation times of an hour or more in standard protocols.IHC analysis on the timescale of tens of minutes could potentially be applied during surgery,enabling clinicians to react in more dynamically and efficiently.Furthermore,this rapid IHC implementation along with conservative tissue usage has strong potential for the implementation of multiplexed assays,allowing the exploration of optimal assay conditions with a small amount of tissue to ensure high-quality staining results for the remainder of the sample.
