The management of the irrigation is fundamental for success of leaves vegetables production. The work was carried out aiming to evaluate the production response of the arugula (Eruca sativa Mill.) under different ir...The management of the irrigation is fundamental for success of leaves vegetables production. The work was carried out aiming to evaluate the production response of the arugula (Eruca sativa Mill.) under different irrigation levels at 25, 31, 37 and 43 days after sowing. The treatments were distributed in randomized blocks design with four replication, and evaluated in split-plot scheme of 4 × 4, with the plots constituted by four irrigation levels equivalent to reference evapotranspiration (ET0) at 50%, 75%, 100% and 125% estimated by Penman-Monteith equation; and the split-plot constituted by days after sowing. The arugula planting was established in November of 2008 with direct sowing in nursery with 0.1 m × 0.2 m per plant, using the cv. "Folha Larga". After the germination phase was carried out crop management necessary to the crop production. It was analyzed: plant height (cm), leaf number, fresh weight (g m2) and dry weight (g m2). The results showed the significant differences at level of 5% of probability for the Scott-Knott test for all variables analyzed after 37 days of planting. The results permit to infer that the irrigation levels exert influence under the studied variables with the higher efficiency of water use found for levels of 75% and 100% of the reference evapotranspiration.展开更多
Soil salinity is among the abiotic stressors that threaten agriculture the most,and purslane(Portulaca oleracea L.)is a dicot species adapted to inland salt desert and saline habitats that hyper accumulates salt and h...Soil salinity is among the abiotic stressors that threaten agriculture the most,and purslane(Portulaca oleracea L.)is a dicot species adapted to inland salt desert and saline habitats that hyper accumulates salt and has high phytoremediation potential.Many researchers consider purslane a suitable model species to study the mechanisms of plant tolerance to drought and salt stresses.Here,a robust salinity stress protocol was developed and used to characterize the morphophysiological responses of young purslane plants to salinity stress;then,leaf tissue underwent characterization by distinct omics platforms to gain further insights into its response to very high salinity stress.The salinity stress protocol did generate different levels of stress by gradients of electrical conductivity at field capacity and water potential in the saturation extract of the substrate,and the morphological parameters indicated three distinct stress levels.As expected from a halophyte species,these plants remained alive under very high levels of salinity stress,showing salt crystal-like structures constituted mainly by Na^(+),Cl^(−),and K^(+)on and around closed stomata.A comprehensive and large-scale metabolome and transcriptome single and integrated analyses were then employed using leaf samples.The multi-omics integration(MOI)system analysis led to a data-set of 51 metabolic pathways with at least one enzyme and one metabolite differentially expressed due to salinity stress.These data sets(of genes and metabolites)are valuable for future studies aimed to deepen our knowledge on the mechanisms behind the high tolerance of this species to salinity stress.In conclusion,besides showing that this species applies salt exclusion already in young plants to support very high levels of salinity stress,the initial analysis of metabolites and transcripts data sets already give some insights into other salt tolerance mechanisms used by this species to support high levels of salinity stress.展开更多
文摘The management of the irrigation is fundamental for success of leaves vegetables production. The work was carried out aiming to evaluate the production response of the arugula (Eruca sativa Mill.) under different irrigation levels at 25, 31, 37 and 43 days after sowing. The treatments were distributed in randomized blocks design with four replication, and evaluated in split-plot scheme of 4 × 4, with the plots constituted by four irrigation levels equivalent to reference evapotranspiration (ET0) at 50%, 75%, 100% and 125% estimated by Penman-Monteith equation; and the split-plot constituted by days after sowing. The arugula planting was established in November of 2008 with direct sowing in nursery with 0.1 m × 0.2 m per plant, using the cv. "Folha Larga". After the germination phase was carried out crop management necessary to the crop production. It was analyzed: plant height (cm), leaf number, fresh weight (g m2) and dry weight (g m2). The results showed the significant differences at level of 5% of probability for the Scott-Knott test for all variables analyzed after 37 days of planting. The results permit to infer that the irrigation levels exert influence under the studied variables with the higher efficiency of water use found for levels of 75% and 100% of the reference evapotranspiration.
基金Funding The authors disclose receipt of the following financial support for the research,authorship,and/or publication of this article:the grant(01.13.0315.00-DendePalm Project)for this study was awarded by the Brazilian Research and Innovation Agency(FINEP).
文摘Soil salinity is among the abiotic stressors that threaten agriculture the most,and purslane(Portulaca oleracea L.)is a dicot species adapted to inland salt desert and saline habitats that hyper accumulates salt and has high phytoremediation potential.Many researchers consider purslane a suitable model species to study the mechanisms of plant tolerance to drought and salt stresses.Here,a robust salinity stress protocol was developed and used to characterize the morphophysiological responses of young purslane plants to salinity stress;then,leaf tissue underwent characterization by distinct omics platforms to gain further insights into its response to very high salinity stress.The salinity stress protocol did generate different levels of stress by gradients of electrical conductivity at field capacity and water potential in the saturation extract of the substrate,and the morphological parameters indicated three distinct stress levels.As expected from a halophyte species,these plants remained alive under very high levels of salinity stress,showing salt crystal-like structures constituted mainly by Na^(+),Cl^(−),and K^(+)on and around closed stomata.A comprehensive and large-scale metabolome and transcriptome single and integrated analyses were then employed using leaf samples.The multi-omics integration(MOI)system analysis led to a data-set of 51 metabolic pathways with at least one enzyme and one metabolite differentially expressed due to salinity stress.These data sets(of genes and metabolites)are valuable for future studies aimed to deepen our knowledge on the mechanisms behind the high tolerance of this species to salinity stress.In conclusion,besides showing that this species applies salt exclusion already in young plants to support very high levels of salinity stress,the initial analysis of metabolites and transcripts data sets already give some insights into other salt tolerance mechanisms used by this species to support high levels of salinity stress.