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Analysis of tumor-draining vein secretome: A direct access to tumor-derived extracellular vesicles in surgical lung cancer patients
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作者 YANGYI HE DAVID SANCHEZ-LORENTE +5 位作者 MELISSA ACOSTA-PLASENCIA MARC BOADA ANGELA GUIRAO RAMON MMARRADES LAUREANO MOLINS ALFONS NAVARRO 《BIOCELL》 SCIE 2023年第5期951-957,共7页
Tumor-secreted extracellular vesicles(EVs)participate in the metastasis process through different mechanisms,including the preparation of the pre-metastatic niche to grant circulating tumor cells(CTCs)implantation and... Tumor-secreted extracellular vesicles(EVs)participate in the metastasis process through different mechanisms,including the preparation of the pre-metastatic niche to grant circulating tumor cells(CTCs)implantation and growth.The study of the metastasis process through the analysis of CTCs and tumor-derived EVs is difficult because of the dilution grade of these elements in peripheral blood.In early-stage lung cancer patients,the tumor-secreted products are even more diluted.An attractive strategy in surgical lung cancer patients is to purify them from a pulmonary tumor-draining vein where they are enriched.The information obtained from the analysis of EVs and CTCs purified from this source could give more accurate information about tumor biology and could be an important source of biomarkers to identify patients at high risk of relapse after curative surgery. 展开更多
关键词 Lung cancer Liquid biopsy Tumor-draining vein Extracellular vesicles EXOSOMES
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Activities of autonomic neurotransmitters in meibomian gland tissues are associated with menopausal dry eye 被引量:1
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作者 Lianxiang Li Dongling Jin +4 位作者 Jinsheng Gao Liguang Wang Xianjun Liu Jingzhang Wang Zhongxin Xu 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第35期2761-2769,共9页
The secretory activities of meibomian glands are regulated by the autonomic nervous system, The change in density and activity of autonomic nerves in meibomian glands during menopause play an important role in the pat... The secretory activities of meibomian glands are regulated by the autonomic nervous system, The change in density and activity of autonomic nerves in meibomian glands during menopause play an important role in the pathogenesis of dry eye. In view of this, we established a dry eye rat model by removing the bilateral ovaries. We used neuropeptide Y and vasoactive intestinal polypeptide as markers of autonomic neurotransmitters. Our results showed that the concentration of estradiol in serum significantly decreased, the density of neuropeptide Y immunoreactivity in nerve fibers significantly increased, the density of vasoactive intestinal polypeptide immunoreactivity in nerve fibers significantly decreased, and the ratio of vasoactive intestinal polypeptide/neuropeptide Y positive staining significantly decreased. These results suggest that a decrease in ovary activity may lead to autonomic nervous system dysfunction, thereby affecting the secretory activity of the meibomian gland, which participates in sexual hormone imbalance-induced dry eye. 展开更多
关键词 OVARIECTOMY meibomian gland meibomian gland dysfunction neuropeptide Y vasoactive intestinalpolypeptide autonomic nervous system dysfunction dry eye sexual hormone rats autonomicnerve neural regeneration
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Different kinds of stem cells in the development of SARS-CoV-2 treatments
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作者 Monica Maribel Mata-Miranda Miguel Sanchez-Brito Gustavo Jesus Vazquez-Zapien 《World Journal of Stem Cells》 SCIE 2021年第5期439-451,共13页
On February 11,2020,the World Health Organization officially announced the coronavirus disease 2019(COVID-19)caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),as an emerging recent pandemic ill... On February 11,2020,the World Health Organization officially announced the coronavirus disease 2019(COVID-19)caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),as an emerging recent pandemic illness,which currently has approximately taken the life of two million persons in more than 200 countries.Medical,clinical,and scientific efforts have focused on searching for new prevention and treatment strategies.Regenerative medicine and tissue engineering focused on using stem cells(SCs)have become a promising tool,and the regenerative and immunoregulatory capabilities of mesenchymal SCs(MSCs)and their exosomes have been demonstrated.Moreover,it has been essential to establishing models to reproduce the viral life cycle and mimic the pathology of COVID-19 to understand the virus's behavior.The fields of pluripotent SCs(PSCs),induced PSCs(iPSCs),and artificial iPSCs have been used for this purpose in the development of infection models or organoids.Nevertheless,some inconveniences have been declared in SC use;for example,it has been reported that SARS-CoV-2 enters human cells through the angiotensin-converting enzyme 2 receptor,which is highly expressed in MSCs,so it is important to continue investigating the employment of SCs in COVID-19,taking into consideration their advantages and disadvantages.In this review,we expose the use of different kinds of SCs and their derivatives for studying the SARS-CoV-2 behavior and develop treatments to counter COVID-19. 展开更多
关键词 COVID-19 SARS-CoV-2 Stem cells EXOSOMES Pluripotent stem cells Mesenchymal stem cells Artificial stem cells
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Polymyxin B effects on motility parameters of cryopreserved bull semen
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作者 Mojtaba Rashedi Mohammad Hashem Fazeli +1 位作者 Hamid Gholami Mohammad Bahreini 《Asian pacific Journal of Reproduction》 2017年第1期38-45,共8页
Objective:To evaluate the effect of adding different values of polymyxin B (PMB) to bull semen on various motility parameters of post-thawed semen such as total motility, progressive motility and velocity parameters u... Objective:To evaluate the effect of adding different values of polymyxin B (PMB) to bull semen on various motility parameters of post-thawed semen such as total motility, progressive motility and velocity parameters using kinetic parameters of sperm by Computer Assisted Sperm Analysis.Methods: Gram negative bacteria release lipopolysaccharide, which induces the apoptotic pathway. Antibiotics are added to semen in order to prevent bacterial contaminations in bovine semen. These antibiotics kill the bacteria especially gram negative bacteria. Therefore, their endotoxins are released during bacteriolysis and bind to the head region and midpiece of sperm. PMB is a bactericidal antibiotic against multidrug resistant gram-negative bacteria and is able to neutralize the toxic effects of the released endotoxin. This study was performed on 3-year old Taleshi bulls. Results:The results showed both positive and negative significant effects of PMB on semen quality. Total motility and progressive motility were significantly increased (P<0.0001) by 100 μg per mL of PMB (55.2% and 48.8% respectively) against the control groups (43.5% and 37.7%, respectively). Moreover, they were significantly decreased (P<0.0001) by 1000 μg per mL of PMB (35.2% and 28.8% respectively) against the control groups (43.5% and 37.7% respectively) in above-mentioned parameters. In Computer Assisted Semen Analyzer, parameter VAP was significantly decreased (P<0.04) in 1000 μg (69.6 μm/s) against the control group (78.7 μm/s). Finally, using PMB in processing cryopreserved bull semen is advised, but before using it, the rate of endotoxins must be measured.Conclusions: We advise using PMB after measuring endotoxin concentration;In vitro,in vivoand in field fertilization, adding other sperm evaluation factors such as acrosomal integrity, DNA integrity, mitochondrial function to PMB treated semen. 展开更多
关键词 Taleshi BULL SEMEN POLYMYXIN B COMPUTER Assist SEMEN ANALYZER MOTILITY parameters
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Epithelial-mesenchymal Plasticity in Breast Cancer Metastasis
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作者 T. Blick C. A. Pinto +12 位作者 D. Huang A. Tachtsidis E. Widodo H. Hugo R. Wafai D. Gunasinghe I. Haviv M. E. Lenburg R. M. Neve D.F. Newgreen M.L. Ackland M. Waltham E. W. Thompson 《中国肺癌杂志》 CAS 2009年第6期I0001-I0002,共2页
Transitions between epithelial and mesenchymal states, which we have termed Epithelial Mesenchymal Plasticity (EMP), are
关键词 肺癌 癌细胞 治疗 疗效
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Unc-51-like kinase(ULK)complex-independent autophagy induced by hypoxia 被引量:1
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作者 Yan Feng Helen H.Kang +3 位作者 Pui-Mun Wong Minghui Gao Ping Wang Xuejun Jiang 《Protein & Cell》 SCIE CAS CSCD 2019年第5期376-381,共6页
Dear Editor,Macroautophagy(referred to as autophagy herein)is an evolutionarily con served,lysosomal degradatio n process by which cells rid themselves of aggregated proteins and damaged organelles(He and Klionsky,200... Dear Editor,Macroautophagy(referred to as autophagy herein)is an evolutionarily con served,lysosomal degradatio n process by which cells rid themselves of aggregated proteins and damaged organelles(He and Klionsky,2009).This process involves a finely orchestrated molecular pathway comprising a plethora of ATG proteins essential for autophagosome formation.The mammalian Unc-51-like kinase(ULK)complex plays an essential role to initiate canonical autophagy pathway by relaying upstream nutrient and stress signals to downstream autophagy machinery(Mizushima,2010;Wong et al.,2013). 展开更多
关键词 Unc-51-like KINASE ULK HYPOXIA
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