Projection-based 3D bioprinting(PBP):a powerful method to fabricate 3D cellular structures Three-dimensional(3D)bioprinting has played an important role in tissue engineering and regenerative medicine areas over the p...Projection-based 3D bioprinting(PBP):a powerful method to fabricate 3D cellular structures Three-dimensional(3D)bioprinting has played an important role in tissue engineering and regenerative medicine areas over the past decade[1].Different from traditional cell cultures in Petri dishes,3D bioprinting can build bionic structures with a better potential to become artificial organ substitutes[2–4].With the development of photocurable biomaterials,the projection-based 3D printing method has been successfully applied in biological research[5,6].展开更多
Introduction Why 3D cell culture urgently needed?Tissue engineering has extremely influenced the development process of basic biological studies and biomedical technology.For quite a long time,the vast majority of the...Introduction Why 3D cell culture urgently needed?Tissue engineering has extremely influenced the development process of basic biological studies and biomedical technology.For quite a long time,the vast majority of these researches have been relying on the experimental results of conventional two-dimensional(2D)cell culture in flask,petri dish or well plate.However,these 2D culture results could be very different with or even totally opposite to the actual situations in vivo where cells grow inside a 3D extracellular matrix(ECM).Therefore,3D cell culture has been playing a more and more significant role because of its higher accuracy and authenticity[1–4].展开更多
As photocrosslinkable materials,methacryloyl-modified hydrogels are widely used as bioinks in tissue engineering.Existing printing methods to use these hydrogels,including changing the viscosity of the material or mix...As photocrosslinkable materials,methacryloyl-modified hydrogels are widely used as bioinks in tissue engineering.Existing printing methods to use these hydrogels,including changing the viscosity of the material or mixing them with other printing components,have been explored,but their application has been limited due to low printing quality or high cost.In addition,the complex operation of bulky equipment restricts the application of these existing printing methods.This study presents a lightweight stereolithography-based three-dimensional(3D)bioprinting system with a smart mechanical and structural design.The developed bioprinter dimensions were 300 mm×300 mm×200 mm and it can be placed on a benchtop.The equipment has a mini bioink chamber to store a small amount of bioink for each printing.We systematically investigated the point-by-point curing process in the 3D bioprinting method,which can print mixed cells accurately and have good biocompatibility.Here,we provide a compact,low-cost stereolithography bioprinting system with excellent biocompatibility for 3D bioprinting with methacryloyl-modified hydrogels.It can be potentially used for drug screening,studying pathological mechanisms,and constructing biological disease models.展开更多
基金the National Natural Science Foundation of China(Nos.U1909218,81827804 and T2121004).
文摘Projection-based 3D bioprinting(PBP):a powerful method to fabricate 3D cellular structures Three-dimensional(3D)bioprinting has played an important role in tissue engineering and regenerative medicine areas over the past decade[1].Different from traditional cell cultures in Petri dishes,3D bioprinting can build bionic structures with a better potential to become artificial organ substitutes[2–4].With the development of photocurable biomaterials,the projection-based 3D printing method has been successfully applied in biological research[5,6].
基金This work was supported by the National Nature Science Foundation of China(Nos.U1909218,81827804).
文摘Introduction Why 3D cell culture urgently needed?Tissue engineering has extremely influenced the development process of basic biological studies and biomedical technology.For quite a long time,the vast majority of these researches have been relying on the experimental results of conventional two-dimensional(2D)cell culture in flask,petri dish or well plate.However,these 2D culture results could be very different with or even totally opposite to the actual situations in vivo where cells grow inside a 3D extracellular matrix(ECM).Therefore,3D cell culture has been playing a more and more significant role because of its higher accuracy and authenticity[1–4].
基金support of National 863 Program(SS2012AA101306)“the 12th Five-Year Plan”,Jiangxi Advantageous Science and Technology Innovation Team Construction Plan(20153BCB24002)+2 种基金Collaborative Innovation Center Project of Intelligent Management Technology and Equipment for Southern Mountain Orchards(G.J.G.Z.[2014]No.60)National Natural Science Foundation of China(2002017018,51805474)the China Postdoctoral Science Foundation(Grant No.2019T120509).
文摘As photocrosslinkable materials,methacryloyl-modified hydrogels are widely used as bioinks in tissue engineering.Existing printing methods to use these hydrogels,including changing the viscosity of the material or mixing them with other printing components,have been explored,but their application has been limited due to low printing quality or high cost.In addition,the complex operation of bulky equipment restricts the application of these existing printing methods.This study presents a lightweight stereolithography-based three-dimensional(3D)bioprinting system with a smart mechanical and structural design.The developed bioprinter dimensions were 300 mm×300 mm×200 mm and it can be placed on a benchtop.The equipment has a mini bioink chamber to store a small amount of bioink for each printing.We systematically investigated the point-by-point curing process in the 3D bioprinting method,which can print mixed cells accurately and have good biocompatibility.Here,we provide a compact,low-cost stereolithography bioprinting system with excellent biocompatibility for 3D bioprinting with methacryloyl-modified hydrogels.It can be potentially used for drug screening,studying pathological mechanisms,and constructing biological disease models.