Objective: To confirm whether Mycoplasma pneumoniae(MP) are present in reproductive tract of STD patients in China. Methods: Application of nested PCR (nPCR) and DNA sequencing to test samples of urethral/vaginal swab...Objective: To confirm whether Mycoplasma pneumoniae(MP) are present in reproductive tract of STD patients in China. Methods: Application of nested PCR (nPCR) and DNA sequencing to test samples of urethral/vaginal swabs with MP culture confirmation of several nPCR positive patients. Results: 74 of 786 STD patients were positive for MP by nPCR, with a rate of 9.4%. Of the 484 male patients, 10.5%were positive, and among the 302 female patients, 7.6%were positive. There was no significant difference between them (P>0.05). Of 12 cases of MP positive samples by nPCR,4 cases were first generation culture-positive, and one ofthem passed to the next generation successfully. DNAsequencing was performed on the nPCR product of oneswab sample and one MP culture isolation. The determinedsequence was identical to the typical MP strain. Conclusion: In China, MP are present in reproductivetract of both male and female STD natients.展开更多
文摘Objective: To confirm whether Mycoplasma pneumoniae(MP) are present in reproductive tract of STD patients in China. Methods: Application of nested PCR (nPCR) and DNA sequencing to test samples of urethral/vaginal swabs with MP culture confirmation of several nPCR positive patients. Results: 74 of 786 STD patients were positive for MP by nPCR, with a rate of 9.4%. Of the 484 male patients, 10.5%were positive, and among the 302 female patients, 7.6%were positive. There was no significant difference between them (P>0.05). Of 12 cases of MP positive samples by nPCR,4 cases were first generation culture-positive, and one ofthem passed to the next generation successfully. DNAsequencing was performed on the nPCR product of oneswab sample and one MP culture isolation. The determinedsequence was identical to the typical MP strain. Conclusion: In China, MP are present in reproductivetract of both male and female STD natients.
