In a recent paper published in Science,Wei et al.report that long-interspersed element-1(LINE1)RNA is a key target of the N6-methyladenosine(m6A)‘eraser’fat mass and obesity-associated protein(FTO).The FTO-LINE1 RNA...In a recent paper published in Science,Wei et al.report that long-interspersed element-1(LINE1)RNA is a key target of the N6-methyladenosine(m6A)‘eraser’fat mass and obesity-associated protein(FTO).The FTO-LINE1 RNA axis is involved in chromatin regulation and is essential for mammalian development.展开更多
Light sheet fluorescence microscopy(LSFM)is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs.However,LSFM can suffer from optical artifacts for which th...Light sheet fluorescence microscopy(LSFM)is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs.However,LSFM can suffer from optical artifacts for which there is no intrinsic solution.The attenuation of light due to absorbing material causes“shadow”artifacts along both the illumination and detection paths.Several approaches have been introduced to reduce this problem,including scanning illumination and multi-view imaging.However,neither of these approaches completely eliminates the problem.If the distribution of the absorbing material is complex,shadows cannot be avoided.We introduce a new approach that relies on multi-modal integration of two very different mesoscopic techniques.Unlike LSFM,optical projection tomography(OPT)can operate in transmission mode to create a voxel map of the 3D distribution of the sample’s optical attenuation.Here,we demonstrate a hybrid instrument(OPTiSPIM)that can quantify this attenuation and use the information to correct the shadow artifacts of LSFM.展开更多
During free exploration,the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation.However,the behavioral dynamics and neural substrates underly...During free exploration,the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation.However,the behavioral dynamics and neural substrates underlying the exploratory behavior remain poorly understood.We developed computational tools to quantify the exploratory behavior and performed in vivo electrophysiological recordings in a large arena in which mice made sequential excursions into unknown territory.Occupancy entropy was calculated to characterize the cumulative and moment-to-moment behavioral dynamics in explored and unexplored territories.Local field potential analysis revealed that the theta activity in the dorsal hippocampus(d HPC)was highly correlated with the occupancy entropy.Individual d HPC and prefrontal cortex(PFC)oscillatory activities could classify various aspects of free exploration.Initiation of exploration was accompanied by a coordinated decrease and increase in theta activity in PFC and d HPC,respectively.Our results indicate that d HPC and PFC work synergistically in shaping free exploration by modulating exploratory traits during emergence and visits to an unknown environment.展开更多
Dear Editor, Deleted in colorectal cancer （DCC） is a single pass transmembrane glycoprotein that was originally identified in humans as a candidate tumor suppressor （Fearon et al., 1990）. DCC belongs to the immuno...Dear Editor, Deleted in colorectal cancer （DCC） is a single pass transmembrane glycoprotein that was originally identified in humans as a candidate tumor suppressor （Fearon et al., 1990）. DCC belongs to the immunoglobulin superfamily, and the extracellular fragment is composed of four immunoglobu- lin-like （Ig-like） domains followed by six fibronectin type III （FN） domains. DCC plays a pivotal role in axon guidance by mediating a combination of attractive and repulsive effects through interactions with the diffusible guidance cue,展开更多
基金P.S.acknowledges funding from the Peter und Traudl Engelhorn StiftungOpen Access funding enabled and organized by Projekt DEAL.
文摘In a recent paper published in Science,Wei et al.report that long-interspersed element-1(LINE1)RNA is a key target of the N6-methyladenosine(m6A)‘eraser’fat mass and obesity-associated protein(FTO).The FTO-LINE1 RNA axis is involved in chromatin regulation and is essential for mammalian development.
基金the European Union’s 7th Framework VIBRANT project(No.228933 of the FP7-NMP)the Sinergia project(CRII3_125477)of the Swiss National Science Foundation(SNSF)+1 种基金support from the Spanish Ministry of Economy and Competitiveness,“Centro de Excelencia Severo Ochoa 2013–2017”from the CERCA Programme/Generalitat de Catalunya.
文摘Light sheet fluorescence microscopy(LSFM)is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs.However,LSFM can suffer from optical artifacts for which there is no intrinsic solution.The attenuation of light due to absorbing material causes“shadow”artifacts along both the illumination and detection paths.Several approaches have been introduced to reduce this problem,including scanning illumination and multi-view imaging.However,neither of these approaches completely eliminates the problem.If the distribution of the absorbing material is complex,shadows cannot be avoided.We introduce a new approach that relies on multi-modal integration of two very different mesoscopic techniques.Unlike LSFM,optical projection tomography(OPT)can operate in transmission mode to create a voxel map of the 3D distribution of the sample’s optical attenuation.Here,we demonstrate a hybrid instrument(OPTiSPIM)that can quantify this attenuation and use the information to correct the shadow artifacts of LSFM.
基金the National Natural Science Foundation of China(32070985)Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(NYKFKT20190018)Shenzhen Key Laboratory of Translational Research for Brain Diseases(ZDSYS20200828154800001)。
文摘During free exploration,the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation.However,the behavioral dynamics and neural substrates underlying the exploratory behavior remain poorly understood.We developed computational tools to quantify the exploratory behavior and performed in vivo electrophysiological recordings in a large arena in which mice made sequential excursions into unknown territory.Occupancy entropy was calculated to characterize the cumulative and moment-to-moment behavioral dynamics in explored and unexplored territories.Local field potential analysis revealed that the theta activity in the dorsal hippocampus(d HPC)was highly correlated with the occupancy entropy.Individual d HPC and prefrontal cortex(PFC)oscillatory activities could classify various aspects of free exploration.Initiation of exploration was accompanied by a coordinated decrease and increase in theta activity in PFC and d HPC,respectively.Our results indicate that d HPC and PFC work synergistically in shaping free exploration by modulating exploratory traits during emergence and visits to an unknown environment.
文摘Dear Editor, Deleted in colorectal cancer （DCC） is a single pass transmembrane glycoprotein that was originally identified in humans as a candidate tumor suppressor （Fearon et al., 1990）. DCC belongs to the immunoglobulin superfamily, and the extracellular fragment is composed of four immunoglobu- lin-like （Ig-like） domains followed by six fibronectin type III （FN） domains. DCC plays a pivotal role in axon guidance by mediating a combination of attractive and repulsive effects through interactions with the diffusible guidance cue,
