RAPD （random amplified polymorphic DNA） markers were performed to fingerprint 20 varieties of maize. Twenty operon primers generated informative RAPD patterns and selected for further RAPD analysis. These 20 primers...RAPD （random amplified polymorphic DNA） markers were performed to fingerprint 20 varieties of maize. Twenty operon primers generated informative RAPD patterns and selected for further RAPD analysis. These 20 primers yielded 291 of main bands, out of which 169 were polymorphic bands across tested varieties. Each selected primer produced between 59 bands （OPC-01） to 142 bands （OPX-04）. Amplification products （DNA amplicons） and fragments size ranged from 260 bp （OPT-06） to 2,365 bp （OPP-05）. The largest number of polymorphic bands （20 bands） was produced by primer OPX-04 while, the lowest number of polymorphic bands （1 band） was produced by primer OPA-03. The primers of the most interest of this purpose were those that produced more variety specific DNA profiles, such as OPD-03, OPE-18, OPF-05, OPL-11 and OPX-04. The primer efficiency ranged from 0.20 in primer OPA-12 to 0.01 in primer OPA-03. The highest polymorphism and value of discrimination in this study were obtained with primers OPX-04, while, the lowest polymorphism and value of discrimination were obtained with primers OPA-03. The lowest genetic distance was 0.1941 between varieties Manlcet and Biotech Bag, while, the highest genetic distance was 0.6433 between varieties Pio 3751 and Buhooth 106. Cluster analysis （phylogenetic tree） by UPGMA （unweighted pair-group method of arithmetic means） based dendrogram revealed that they were four main genetic groups. The overall analysis of the results reveals that the genetic relationships among the maize varieties were related to some of their morphological characters as well as to their geographical origins at the molecular genetics.展开更多
Novel 3-substituted pyridothienopyrimidine derivatives have been synthesized via the reaction of 2-{7,9-dimethyl-4-oxopyrido[3′,2′：4,5]thieno[3,2-d]pyrimidin-3（4H）-yl}acetohydrazide（5） with a variety of activer...Novel 3-substituted pyridothienopyrimidine derivatives have been synthesized via the reaction of 2-{7,9-dimethyl-4-oxopyrido[3′,2′：4,5]thieno[3,2-d]pyrimidin-3（4H）-yl}acetohydrazide（5） with a variety of activereagents and chemicals. Structures of the newly synthesized compounds were established based on spectral data.The resulting pyridothienopyrimidine derivatives were evaluated for their possible antimicrobial activity and some ofthem represent a new class of potentially antimicrobial compounds, especially compounds 9 and 18 which displayedthe highest activity against Gram-positive bacteria, Gram-negative bacteria, and Fungi in MIC range of 0.12--1.95 μg/mL.展开更多
The present study is concerned with construct two new semidynamical systems which are generated by two partial differential equations of Lasota type. In addition, this study discusses the asymptotic properties： Stron...The present study is concerned with construct two new semidynamical systems which are generated by two partial differential equations of Lasota type. In addition, this study discusses the asymptotic properties： Strong stability, exponential stability, periodic points, the density of periodic points, transitivity and chaos in two spaces： Lp space and Lp space.展开更多
文摘RAPD （random amplified polymorphic DNA） markers were performed to fingerprint 20 varieties of maize. Twenty operon primers generated informative RAPD patterns and selected for further RAPD analysis. These 20 primers yielded 291 of main bands, out of which 169 were polymorphic bands across tested varieties. Each selected primer produced between 59 bands （OPC-01） to 142 bands （OPX-04）. Amplification products （DNA amplicons） and fragments size ranged from 260 bp （OPT-06） to 2,365 bp （OPP-05）. The largest number of polymorphic bands （20 bands） was produced by primer OPX-04 while, the lowest number of polymorphic bands （1 band） was produced by primer OPA-03. The primers of the most interest of this purpose were those that produced more variety specific DNA profiles, such as OPD-03, OPE-18, OPF-05, OPL-11 and OPX-04. The primer efficiency ranged from 0.20 in primer OPA-12 to 0.01 in primer OPA-03. The highest polymorphism and value of discrimination in this study were obtained with primers OPX-04, while, the lowest polymorphism and value of discrimination were obtained with primers OPA-03. The lowest genetic distance was 0.1941 between varieties Manlcet and Biotech Bag, while, the highest genetic distance was 0.6433 between varieties Pio 3751 and Buhooth 106. Cluster analysis （phylogenetic tree） by UPGMA （unweighted pair-group method of arithmetic means） based dendrogram revealed that they were four main genetic groups. The overall analysis of the results reveals that the genetic relationships among the maize varieties were related to some of their morphological characters as well as to their geographical origins at the molecular genetics.
文摘Novel 3-substituted pyridothienopyrimidine derivatives have been synthesized via the reaction of 2-{7,9-dimethyl-4-oxopyrido[3′,2′：4,5]thieno[3,2-d]pyrimidin-3（4H）-yl}acetohydrazide（5） with a variety of activereagents and chemicals. Structures of the newly synthesized compounds were established based on spectral data.The resulting pyridothienopyrimidine derivatives were evaluated for their possible antimicrobial activity and some ofthem represent a new class of potentially antimicrobial compounds, especially compounds 9 and 18 which displayedthe highest activity against Gram-positive bacteria, Gram-negative bacteria, and Fungi in MIC range of 0.12--1.95 μg/mL.
文摘The present study is concerned with construct two new semidynamical systems which are generated by two partial differential equations of Lasota type. In addition, this study discusses the asymptotic properties： Strong stability, exponential stability, periodic points, the density of periodic points, transitivity and chaos in two spaces： Lp space and Lp space.
