AIM: To analyse the role of genetic susceptibility and environmental factors in the process of esophageal cancer (EC) formation in Xi'an, China. METHODS: A hospital based case-control study, combined with molecula...AIM: To analyse the role of genetic susceptibility and environmental factors in the process of esophageal cancer (EC) formation in Xi'an, China. METHODS: A hospital based case-control study, combined with molecular epidemiological method, was carded out. A total of 127 EC cases and 101 controls were interviewed with questionnaires containing demographic items, habit of tobacco smoking, alcohol drinking, and family history of EC. Polymorphism of CYPIA1 and GSTM1 of 127 EC cases and 101 controls were detected by PCR method. The interactions between genetic susceptibility and environmental factors were also discussed. RESULTS: Tobacco smoking, alcohol drinking and a family history of EC were risk factors for EC with an OR of 2.04 (95% CI 1.15-3.60), 3.45(95% CI 1.74-6.91), 3.14 (95% CI 1.28-7.94), respectively. Individuals carrying CYP1A1 Val/Val genotype compared to those with CYP1A1 Ile/Ile genotype had an increased risk for EC (OR 3.35, 95% CI 1.49-7.61). GSTM1 deletion genotype was a risk factor for EC (OR1.81, 95% CI 1.03-3.18). Gene-environment interaction analysis showed that CYPIA1 Val/Val genotype, GSTM1 deletion genotype had synergetic interactions with tobacco smoking, alcohol drinking and family history of EC. CONCLUSION: Tobacco smoking, alcohol drinking and a family history of EC are risk factors for EC. CYP1A1 Val/Val and GSTM1 deletion genotypes are genetic susceptibility biomarkers for EC. There are synergic interactions between genetic susceptibility and environmental factors.展开更多
Aluminum (Al) exposure and apoptotic cell death have been implicated in several neurodegenerative diseases.the mechanisms by which Al interacts with the nervous system are only partly understood.In this study,we used ...Aluminum (Al) exposure and apoptotic cell death have been implicated in several neurodegenerative diseases.the mechanisms by which Al interacts with the nervous system are only partly understood.In this study,we used cultured cortical neurons to investigate the ability of Al to induce the apoptosis of neurons and to explore the role of SAPK/JNK signal transduction pathway on the apoptosis induced by Al.It was found that Al-induced degeneration of cortical neurons involved the DNA fragmentation characteristic of apoptosis.The rate of apoptosis increased significantly,which was measured by TdT-mediated dUTKP nick end labeling.Westerm blot analysis showed that SAPK/JNK activities of cortical neurons varied when the dose and exposure time of AlCl3 were different.Our study demonstrates that Al can induce the apoptosis of cortical neurons and SAPK/JNK signal transduction pathway may play a great role in the apoptosis.展开更多
Polymorphisms in O^6-methylguanine-DNA-methyltransferase(MGMT) gene in the normal Han People of South China was searched by PCRSSCP-DNA sequencing.The result showed that exons 3 and 4 in MGMT gene in the normal Han pe...Polymorphisms in O^6-methylguanine-DNA-methyltransferase(MGMT) gene in the normal Han People of South China was searched by PCRSSCP-DNA sequencing.The result showed that exons 3 and 4 in MGMT gene in the normal Han people of South China existed in polymorphisms,which were both heterozygous mutation.The frequencies of CC,CT genotype in exon 3 were 89.0% and 11.0%,respectively.The frequencies of CC,CT genotype in exon 4 were 95.0% and 5.0%,respectively.The both frequencies of genotype were in accord with Hardy-Weinbarg rule(P>0.05).展开更多
The tetravalent metal salts of monoalkyl phosphates [M(O_3POR)_2] are a new kind of stationary phases of Chromatography-homogeneous bonded phases. This paper deals with the application of cerium bis (monomyristylphosp...The tetravalent metal salts of monoalkyl phosphates [M(O_3POR)_2] are a new kind of stationary phases of Chromatography-homogeneous bonded phases. This paper deals with the application of cerium bis (monomyristylphosphate ) as support to reversed phase liquid chromatography. The results show that the best mobil phase is CH_3CN : H_2 O = 95 : 5. The good separation to the mixture containing six aromatic hydrocarbons and the determination of naphthalene in a group samples have been achieved. The regression analysis shows that detect limits, linearities and precision for six aromatic hydrocarbons are good.展开更多
基金Supported by the National Natural Science Foundation of China,No.39670651
文摘AIM: To analyse the role of genetic susceptibility and environmental factors in the process of esophageal cancer (EC) formation in Xi'an, China. METHODS: A hospital based case-control study, combined with molecular epidemiological method, was carded out. A total of 127 EC cases and 101 controls were interviewed with questionnaires containing demographic items, habit of tobacco smoking, alcohol drinking, and family history of EC. Polymorphism of CYPIA1 and GSTM1 of 127 EC cases and 101 controls were detected by PCR method. The interactions between genetic susceptibility and environmental factors were also discussed. RESULTS: Tobacco smoking, alcohol drinking and a family history of EC were risk factors for EC with an OR of 2.04 (95% CI 1.15-3.60), 3.45(95% CI 1.74-6.91), 3.14 (95% CI 1.28-7.94), respectively. Individuals carrying CYP1A1 Val/Val genotype compared to those with CYP1A1 Ile/Ile genotype had an increased risk for EC (OR 3.35, 95% CI 1.49-7.61). GSTM1 deletion genotype was a risk factor for EC (OR1.81, 95% CI 1.03-3.18). Gene-environment interaction analysis showed that CYPIA1 Val/Val genotype, GSTM1 deletion genotype had synergetic interactions with tobacco smoking, alcohol drinking and family history of EC. CONCLUSION: Tobacco smoking, alcohol drinking and a family history of EC are risk factors for EC. CYP1A1 Val/Val and GSTM1 deletion genotypes are genetic susceptibility biomarkers for EC. There are synergic interactions between genetic susceptibility and environmental factors.
文摘Aluminum (Al) exposure and apoptotic cell death have been implicated in several neurodegenerative diseases.the mechanisms by which Al interacts with the nervous system are only partly understood.In this study,we used cultured cortical neurons to investigate the ability of Al to induce the apoptosis of neurons and to explore the role of SAPK/JNK signal transduction pathway on the apoptosis induced by Al.It was found that Al-induced degeneration of cortical neurons involved the DNA fragmentation characteristic of apoptosis.The rate of apoptosis increased significantly,which was measured by TdT-mediated dUTKP nick end labeling.Westerm blot analysis showed that SAPK/JNK activities of cortical neurons varied when the dose and exposure time of AlCl3 were different.Our study demonstrates that Al can induce the apoptosis of cortical neurons and SAPK/JNK signal transduction pathway may play a great role in the apoptosis.
文摘Polymorphisms in O^6-methylguanine-DNA-methyltransferase(MGMT) gene in the normal Han People of South China was searched by PCRSSCP-DNA sequencing.The result showed that exons 3 and 4 in MGMT gene in the normal Han people of South China existed in polymorphisms,which were both heterozygous mutation.The frequencies of CC,CT genotype in exon 3 were 89.0% and 11.0%,respectively.The frequencies of CC,CT genotype in exon 4 were 95.0% and 5.0%,respectively.The both frequencies of genotype were in accord with Hardy-Weinbarg rule(P>0.05).
文摘The tetravalent metal salts of monoalkyl phosphates [M(O_3POR)_2] are a new kind of stationary phases of Chromatography-homogeneous bonded phases. This paper deals with the application of cerium bis (monomyristylphosphate ) as support to reversed phase liquid chromatography. The results show that the best mobil phase is CH_3CN : H_2 O = 95 : 5. The good separation to the mixture containing six aromatic hydrocarbons and the determination of naphthalene in a group samples have been achieved. The regression analysis shows that detect limits, linearities and precision for six aromatic hydrocarbons are good.
