This work summarizes recently published information on the solar UV broadband irradiation of Pernambuco, Northeast of Brazil. We describe the spatial and temporal distribution of solar UV radiation and its relationshi...This work summarizes recently published information on the solar UV broadband irradiation of Pernambuco, Northeast of Brazil. We describe the spatial and temporal distribution of solar UV radiation and its relationship with climatic and geographical conditions. Statistical experimental correlation between solar total irradiation and UV broadband obtained for 03 locations was generalized by the use of Koppen-Geiger Climatic criterium, which was used for mapping the spatial/temporal distribution of broadband UV. The climatological solar radiations used in the correlations were obtained by modeling through satellite and previously verified with terrestrial data. We present one map with the location of the recording stations where the statistical correlations were measured, one annual and 12 monthly contour maps describing monthly daily solar UV radiation levels throughout the territory of Pernambuco. The solar UV irradiation (“broadband”) annual-average daily value in the State of Pernambuco varied from 226 to 268 Wh/m<sup>2</sup>. Seasonal variation of solar UV irradiation in the State of Pernambuco follows, in general and as expected, the climate, relief and seasons of the year. The highest value of monthly solar UV irradiation was observed in the central south region of the state, more precisely in Belém do São Francisco, Floresta, Ibimirim and Buíque in the month of December (summer), with 311.8 Wh/m<sup>2</sup>. The lower value was found in the south Agreste region, in Garanhuns and Caruaru, in the month of June (winter), with 162.2 Wh/m<sup>2</sup>.展开更多
The aim of the present study was to evaluate different acaricide treatments for the control of Boophilus microplus on field-kept dairy cattle in the state of Pernambuco, Brazil. The first phase of the experiment consi...The aim of the present study was to evaluate different acaricide treatments for the control of Boophilus microplus on field-kept dairy cattle in the state of Pernambuco, Brazil. The first phase of the experiment consisted of collecting the ingurgitated female Boophilus microplus directly from the hosts for attainment of larvae. After the attainment of larvae, the artificial infestation procedure began on the cattle, with larvae ages ranging from 7 to 14 days. On Day 0, animals were separated into 12 groups to receive the corresponding treatment: Abamectin;Ivermectin;Ivermectin LA;Amitraz;Amitraz + Ivermectin;Amitraz + Ivermectin LA;Amitraz + Abamectin;Association (Cypermethrin + Chlorpyrifos + Citronella);Association + Ivermectin;Association + Ivermectin LA;Association + Abamectin;and Control. Subsequent evaluations were made on post-treatment days +7, +14, +21, +28, +35, +42, +49, +56 and +63. Analyzing the post-treatment effectiveness per day, indices revealed considerable variation ranging from 0% to 96.63%. Such indices demonstrate the significant reduction in the number of ticks on the animals in some groups, especially in the Abamectin group. The analysis of the results demonstrates that the use of different avermectines can assist in the development of Boophilus microplus control programs, thereby reducing the number of acaricide applications and production costs related to ticks.展开更多
The greater use of crop fields by increasing plant population is an alternative to minimize losses to the farmers who use crop intercropped provides other benefits such as lower incidence of diseases and pests, and be...The greater use of crop fields by increasing plant population is an alternative to minimize losses to the farmers who use crop intercropped provides other benefits such as lower incidence of diseases and pests, and better exploitation and enrichment agroecosystem. The objective of this study was to evaluate the effect of the population of peanuts with and without inoculation and intercropping with forage palm on growth, productivity and biomass peanut. The treatments consisted of different populations of peanut (one, two and three rows of peanut row spacing of two meters of cactus pear), with and without inoculant, analyzed in subdivided plot scheme (3 × 2), totalizing six treatments in a randomized block design with four replications. The variables evaluated were plant height, number of branches, shoot dry mass and root nodule number, nodule dry weight, biomass productivity and legumes. The largest population of plants in number of lines of peanut intercropped with forage cactus promotes greater total biomass production and yield of beans and does not alter the height, branch number, dry mass of aerial part roots and nodules. The inoculant application did not result in to increased productivity biomass and growth of peanut plants, their use is not indicated. The planting of a row of peanuts between the rows of palm is not economically recommended.展开更多
Isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to host.This study evaluated the effects of goat yogu...Isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to host.This study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains,as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in vitro.Goat yogurts with IG ingredients(IGI)stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation,as well as decreased the pH values and enhanced the organic acid production.Goat yogurts with IGI increased the population of Lactobacillus spp.and Bifidobacterium spp.during a 24-h in vitro colonic fermentation.A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation,being similar to the effect caused by fructooligosaccharides.Goat yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic fermentation.Goat yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.展开更多
The Caatinga biome is an important ecosystem in the semi-arid region of Brazil.It has significantly degraded due to human activities and is currently a region undergoing desertification.Thus,monitoring the variation i...The Caatinga biome is an important ecosystem in the semi-arid region of Brazil.It has significantly degraded due to human activities and is currently a region undergoing desertification.Thus,monitoring the variation in the Caatinga biome has become essential for its sustainable development.However,traditional methods for estimating aboveground biomass(AGB)are time-consuming and destructive.Remote sensing,such as optical and radar imaging,can estimate and correlate with vegetation.Nevertheless,radar imaging is still a novelty to be applied in estimating the AGB of this biome,which is an area with little research.Therefore,this study aimed to use Sentinel-1 images to estimate the AGB of the Caatinga biome in Sergipe State(northeastern Brazil)and to verify its influencing factors.Nineteen sample plots(30 m×30 m)were selected,and the stems of individuals with a circumference at breast height(1.3 m above the ground)equal to or greater than 6.0 cm were measured,and the AGB through an allometric equation was estimated.The Sentinel-1 images from 3 different periods(green,intermediate,and dry periods)were used to consider the phenological conditions of the Caatinga biome.All the pre-processing and extraction of attributes(co-polarized VV(vertical transmit and vertical receive),cross-polarized VH(vertical transmit and horizontal receive),and band ratio VH/VV backscatter,radar vegetation index,dual polarization synthetic aperture radar(SAR)vegetation index(DPSVI),entropy(H),and alpha angle(α))were performed with Sentinel’s Application Platform.These attributes were used to estimate the AGB through simple and multiple linear regressions and evaluated by the coefficients of determination(R2),correlation(r),and root mean squared error(RMSE).The results showed that the attributes individually had little ability to estimate the AGB of the Caatinga biome in the three periods.Combined with multiple regression,we found that the intermediate period presented the equation with the best results among the observed and estimated variables(R^(2)=0.73;r=0.85;RMSE=8.33 Mg/hm^(2)),followed by the greenness period(R2=0.72;r=0.85;RMSE=8.40 Mg/hm^(2)).The attributes contributing to these equations were VH/VV,DPSVI,H,α,and co-polarized VV for the green period and cross-polarized VH for the intermediate period.The study showed that the Sentinel-1 images could be used to estimate the AGB of the Caatinga biome in the green and intermediate phenological periods since the SAR attributes highly correlated with the estimated variable(i.e.,AGB)through multiple linear equations.展开更多
Enterococcus faecalis is a Gram-positive bacterium commonly found in the gastrointestinal tract that can cause serious infections. Many enterococci have broad resistance to antibiotics including penicillin, cephalospo...Enterococcus faecalis is a Gram-positive bacterium commonly found in the gastrointestinal tract that can cause serious infections. Many enterococci have broad resistance to antibiotics including penicillin, cephalosporins, aminoglycosides and glycopeptides. There are several adaptation mechanisms that bacteria can undergo to become more resistant, among them is the formation of biofilm. Several genes have been linked to the increase in the capacity of biofilm formation by bacteria such as gelE, esp and asa1. The aim of this research was to evaluate the biofilm formation of 12 E. faecalis isolates collected in hospitals and a standard strain, as well as to evaluate the hydrophobicity of its membrane and the presence of virulence genes. All the isolates formed biofilm and the characteristics of their membrane were variable. In addition, the presence of at least one virulence gene was found in all the 12 isolates, and none of the genes in the standard strain, indicating the acquisition of these genes in the hospital environment. With this, we can conclude that there is a close relationship between biofilm formation, acquisition of antibiotic resistance and the presence of virulence genes.展开更多
Contextualization: Emptying the bladder is a challenging problem for the urological community. Intermittent catheterization is the most widely used method to restore bladder emptying mechanism. However, this procedure...Contextualization: Emptying the bladder is a challenging problem for the urological community. Intermittent catheterization is the most widely used method to restore bladder emptying mechanism. However, this procedure can have a negative impact on self-image and result in a decline in the quality of life of patients. In this context, the use of a bladder emptying device (SVCATH3D) proposes to be effective and have a positive impact on the quality of life of different patients. Objective: The objective of the study was to evaluate the functionality of a new device for both intermittent and controlled emptying of the bladder in both sexes and ages. Materials and methods: A randomized clinical trial was conducted with 251 patients, with different bladder problems, from March 2013 to January 2023. After randomization, the patients were divided into two groups: Group I (SVCATH3D) and Group II (Clean Intermittent Catheterization). The primary outcome was defined as the impact on quality of life. Data on episodes of urinary tract infection, adverse effects, number of diaper use and treatment costs were analyzed. Results: The apposition of the SVCATH3D was performed on an outpatient basis, with no complications during the procedures. The patients were followed up for 10 years. There was a significant improvement in quality of life when comparing the moments before and after the use of SVCATH3D (p Conclusion: The study using SVCATH3D showed promising results in relation to functionality, showing improvement in quality of life with a reduction in episodes of urinary infection and amount of diapers/day. This allows us to conclude that SVCATH3D can represent an important step in the treatment of patients suffering from various bladder problems both incontinence and affecting emptying mechanism.展开更多
The use of medicinal plants as therapeutic and economic resources has been on the rise in recent years. In Brazil, however, increasing doubt is being cast on the quality of these products, owing to the prevalence of a...The use of medicinal plants as therapeutic and economic resources has been on the rise in recent years. In Brazil, however, increasing doubt is being cast on the quality of these products, owing to the prevalence of adulteration and fraud. Solar radiation can cause serious damage to human skin, as a result, mostly, of ultraviolet light, which is a cause of skin cancer. Photoprotective substances are capable of absorbing, reflecting, or refracting ultraviolet radiation and thus protecting skin from exposure to sunlight. The present study aimed to characterize samples and examine the phytochemical profile and photoprotective potential of bark and leaves of Erythrina velutina Willd. The samples underwent five extraction methods using 80% ethanol. The phenolic content was measured using spectrophotometry. Antioxidant activity was examined using the DPPH and the photoprotective properties of the plant extracts were assessed using the method developed by Mansur. There was a quantitative difference in some groups of metabolites, with higher levels of tannin in the bark and of flavonoids in the leaves. The latter showed greater DPPH free radical scavenging capacity than the bark, although higher levels of SPF were obtained from the bark, with no statistically significant differences between methods. The results indicate that Erythrina velutina Willd. has potential as a photoprotector.展开更多
BACKGROUND The association of cardiovascular risk burden with disability is unclear.We examined the association between trajectories of the Framingham general cardiovascular disease risk score(FGCRS)with the trajector...BACKGROUND The association of cardiovascular risk burden with disability is unclear.We examined the association between trajectories of the Framingham general cardiovascular disease risk score(FGCRS)with the trajectories of limitations of physical function in older adults.METHODS A total of 1219 participants with no disabilities from the International Mobility in Aging Study(IMIAS)study who had up to three repeated measures of FGCRS between 2012–2016 and without a history of stroke or coronary heart disease at baseline and follow-up were included.FGCRS at baseline was assessed and categorized into tertiles.Physical function was evaluated with the Short Physical Performance Battery(SPPB).The data were analyzed using linear mixed-effects models.RESULTS At baseline,FGCRS ranged between 3–94(mean score:24±15.8),participants were 32(2.6%),502(41.2%)and 685(56.2%)in lowest,middle,and highest tertiles,respectively.In the trajectories of limitations of physical function,the lowest FGCRS had no differences,while the middle and highest had a decrease in physical performance between 2012–2014(P=0.0001).Age,being female,living in Andes Mountains,having middle and highest FGCRS,higher alcohol consumption,being obese,lack of exercise and cognitive impairment increase the probability of disability(P<0.05).Alternatively,living in more developed regions and having a higher educational level reduced the probability of disability during the follow-up time(P<0.05).CONCLUSIONS Higher cardiovascular risk burden is associated with decreased physical performance,especially in gait.Results suggest SPPB may provide a measure of cardiovascular health in older adults.展开更多
Since the first reports of coronavirus disease 2019(COVID-19)cases in December 2019 in China,numerous papers have been published describing a high frequency of liver injury associated with severe acute respiratory syn...Since the first reports of coronavirus disease 2019(COVID-19)cases in December 2019 in China,numerous papers have been published describing a high frequency of liver injury associated with severe acute respiratory syndrome coronavirus 2 infection,many of them proposing a link between these findings and patient outcomes.Increases in serum aminotransferase levels(ranging from 16%to 62%)and bilirubin levels(ranging from 5%to 21%)have been reported and seem to be more often observed in patients with severe forms of COVID-19.Although absolute changes in these parameters are frequently seen,other variables,such as the ratio above the upper limit of normal,the onset of liver injury as a complication in severe cases and histopathological findings,reinforce that liver changes are of dubious clinical relevance in the course of this disease.Other factors must also be considered in these analyses,such as the repercussions of hemodynamic changes,the presence of thrombotic events,and,mainly,the possible drug-induced liver injury with the current,yet off-label,treatment.This paper aimed to analyze the currently available data on liver injury in patients with COVID-19.展开更多
Oxidative stress,inflammation,and gut microbiota impairments have been implicated in the development and maintenance of diabetes mellitus.Strategies capable of recovering the community of commensal gut microbiota and ...Oxidative stress,inflammation,and gut microbiota impairments have been implicated in the development and maintenance of diabetes mellitus.Strategies capable of recovering the community of commensal gut microbiota and controlling diabetes mellitus have increased in recent years.Some lactobacilli strains have an antioxidant and anti-inflammatory system capable of protecting against oxidative stress,inflammation,and diabetes mellitus.Experimental studies and some clinical trials have demonstrated that Limosilactobacillus fermentum strains can beneficially modulate the host antioxidant and anti-inflammatory system,resulting in the amelioration of glucose homeostasis in diabetic conditions.This review presents and discusses the currently available studies on the identification of Limosilactobacillus fermentum strains with anti-diabetic properties,their sources,range of dosage,and the intervention time in experiments with animals and clinical trials.This review strives to serve as a relevant and well-cataloged reference of Limosilactobacillus fermentum strains capable of inducing anti-diabetic effects and promoting health benefits.展开更多
Objective: To explore the effects of aqueous extract from Phthirusa pyrifolia leaves(67 mg/kg body weight for 12 days) on the reproductive function of male Wistar rats through oral administration.Methods: Animals(n = ...Objective: To explore the effects of aqueous extract from Phthirusa pyrifolia leaves(67 mg/kg body weight for 12 days) on the reproductive function of male Wistar rats through oral administration.Methods: Animals(n = 30), aged 13 weeks and weighing(378.5 ± 5.0) g, were housed in a vivarium under controlled environmental conditions [photoperiod of 12 h light/dark,temperature of(23 ± 1)C] and were fed standard rations ad libitum. The experiment ran for 12 days, wherein animals were divided into three groups: negative control(n = 6)received water, positive control(n = 12) with finasteride at a concentration of 1.0 mg/kg;and a test group(n = 12) submitted to aqueous extract. At the end of the experiment, the animals were sacrificed and submitted to analyses.Results: The morphological results of the testes showed that the aqueous extract induced significant changes in the diameter and cross-sectional area of the seminiferous tubules as well as the thickness of the seminiferous epithelium. Furthermore, the extract was able to abruptly decrease testosterone concentrations by about 81.88% in the treated group when compared with the negative control,(47.0 ± 4.8) ng/d L and(255.0 ± 2.0) ng/d L,respectively, and 76.8%,(211.0 ± 8.7) ng/d L, when compared with finasteride. However,the extract causes neither liver damage nor impairment of renal function.Conclusions: These results suggest that the high amounts of flavonoids shown to be in the extract may be responsible for its hepato-protective effects and suggest a possible decrease in the libido and reproduction of rats.展开更多
The main objective of this paper was to evaluate the daily actual evapotranspiration (ET) accuracy obtained by remote sensing algorithms when compared with Bowen ratio measurements performed in the cotton fields. The ...The main objective of this paper was to evaluate the daily actual evapotranspiration (ET) accuracy obtained by remote sensing algorithms when compared with Bowen ratio measurements performed in the cotton fields. The experiment was conducted in a cotton experimental field of EMBRAPA located in Ceará State, Brazil. Seven TM Landsat-5 images acquired in 2005 and 2008 were used to perform SEBAL and SSEB algorithms. The comparison between the estimated values by remoting sensing algorithms and the measured values in situ showed an acceptable accuracy. Besides, SSEB algorithm showed to be an important tool for ET analysis in the semi-arid regions, due to the fact that it does not need the meteorological data to solve the energy balance, but only the average temperature of the “hot” and “cold” pixels. Additionally, SSEB presents simpler processing than SEBAL algorithm that needs to solve an iterative process to obtain the sensible heat flux values.展开更多
Objective: The study aims to evaluate the use of a teaching method proposed by Paulo Freire, Culture Circles, in the education of teenagers multipliers on leprosy awareness. Methods: It is an action-research study wit...Objective: The study aims to evaluate the use of a teaching method proposed by Paulo Freire, Culture Circles, in the education of teenagers multipliers on leprosy awareness. Methods: It is an action-research study with a qualitative approach developed in a public school in Pernambuco, Brazil. Five Culture Circles were conducted involving the participation of 26 teenagers. The followings were used as data collection tools: observation, field notes, photography and filming. Results: The educational intervention on health addressed the following topics: 1) Definition and transmission of leprosy;2) Characteristics and diagnosis of leprosy;3) Treatment of leprosy;4) Aesthetics, prejudice and mental health related to leprosy;and 5) Planning of educational activities for teenagers as health multipliers on leprosy awareness. The educational action on health provided this age group to perceive themselves, act as political subjects in the development of Culture Circles, and act as protagonists in the dissemination of knowledge on leprosy. Conclusions: This study highlights that the application of active methodologies, such as Culture Circles, is able to encourage the engagement of young people in community empowerment and bring together health professionals and the school community in an intersectoral work in order to develop action strategies involving the promotion of health in the context of neglected diseases such as leprosy.展开更多
Sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architect...Sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architecture and biomass. The expectation is also an increase in sugarcane biomass. The study hypothesized that high N rates applied to sugarcane fields increases root growth and N stored in roots, promoting higher biomass and N accumulated in shoots. Two experiments were set up in Southeastern Brazil, on a Typic Kandiudox (TK) and Rhodic Eutrudox (RE). Four treatments were studied 1) N application in the plant-cane (0 and 120 kg·ha-1 N) and 2) N application in the ratoon (0 and 150 kg·ha-1 N). The shoot biomass and the root density (by the core method up to 0.6 m) were evaluated over the first ratoon crop cycle, and the N content in those compartments was also examined. There was no carry over effect on N applied at planting in root and shoot biomass in the ratoon crop cycle. At the RE site, the ratoon N fertilization increased root density in the superficial soil layer (0 - 0.2 m) and close to the plants (<0.3 m). The effect of N addition on root biomass, and biomass and N accumulated in shoot was limited in both sites. Increasing N rates in unburned sugarcane fields do not consistently increases root and shoot biomass under Brazilian field conditions.展开更多
In this paper, data analysis and modeling of gamma ray tomography taken into account spatial resolution and source of errors and the attenuation coefficient measurement in row data from tomography process are presente...In this paper, data analysis and modeling of gamma ray tomography taken into account spatial resolution and source of errors and the attenuation coefficient measurement in row data from tomography process are presented. The results showed that this method is simple, effective and should be prior to any data treatment for opaque vessel reactor and by reconstruction algorithm in process imaging.展开更多
The objective of this work is to assess the impacts of IPCC AR5 climate change scenarios on water resources and hydrological processes across the entire Brazilian territory. Hydrological simulations are carried out in...The objective of this work is to assess the impacts of IPCC AR5 climate change scenarios on water resources and hydrological processes across the entire Brazilian territory. Hydrological simulations are carried out in total drainage area of about 11,535,645 km<sup>2</sup> and average stream flow of about 272,460 m<sup>3</sup>/s. The study area consists of different climates and land covers such as the Amazon Forest, Northeast Semiarid, Brazilian Savannah, Pantanal wetlands and temperate climate in the South. The atmospheric forcing to drive the large-scale hydrological model MGB-IPH is derived from the downscaling of two global climate models, HadGEM2-ES and MIROC5, by the Eta Regional Climate Model, at 20 km resolution. The Eta model provided the downscaling of the baseline (1961-1990) and three time-slices (2011-2040, 2041-2070 and 2071-2099). These projections adopted two emission scenarios, the RCP 4.5 and RCP 8.5. The change in the average and extremes of precipitation, evapotranspiration, rates of river discharge and soil moisture were assessed. The simulations showed the response of the hydrographic regions due to change of precipitation and potential evapotranspiration in the scenarios. Water availability decreases in almost the entire study area (exception for the South) and the major basins for hydroelectric power generation are affected. The Northwest, Amazon and a small area along the Northeast Atlantic coast exhibited intensification of the extremes discharges, where the anomaly is positive for high-flow (Q<sub>10</sub>) and negative for low-flow (Q<sub>95</sub>). The results highlight the most climatic sensitive regions in Brazil in terms of hydrological variables and water resources.展开更多
Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal cells.Methods:The saline ext...Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal cells.Methods:The saline extract was prepared,with NaCl(0.15 M),by constant stirring of the dried and pulverized leaves,followed by volume reduction by lyophilization.The extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was performed.The antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice splenocytes.Results:Fifteen compounds were identified belonging to two main classes terpenoids and phenolics.The extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of flavonoid.Moreover,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these cells.Conclusions:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.展开更多
BACKGROUND Occult hepatitis C virus(HCV) infection(OCI) may be associated with extrahepatic diseases and it is known that the patients with chronic kidney disease(CKD) who are on hemodialysis(HD) present a higher prev...BACKGROUND Occult hepatitis C virus(HCV) infection(OCI) may be associated with extrahepatic diseases and it is known that the patients with chronic kidney disease(CKD) who are on hemodialysis(HD) present a higher prevalence of this type of infection than the general population, with a worse clinical outcome.However, there are no data in the literature to assess the presence of OCI in patients prior to the initiation of renal replacement therapy(RRT). Therefore, this study aimed to evaluate the occurrence and epidemiological aspects of OCI in patients with Predialysis CKD. We hypothesize that this infection could occur before RRT initiation.AIM To research the status in predialysis patients when HD patients have high prevalence of OCI.METHODS A cross-sectional study was conducted between 2015 and 2017. Adults with creatinine clearance < 60 mL/min·1.73 m^2(predialysis patients) were recruited to the study. Pregnant and postpartum women, patients with glomerulopathies,and patients showing positivity for serological markers of hepatitis B virus(HBV), HCV or human immunodeficiency virus infection were excluded. Patients were diagnosed with OCI according to test results of anti-HCV antibody negativity and HCV RNA positivity in either ultracentrifuged serum or, if serumnegative, in peripheral blood mononuclear cells.RESULTS Among the 91 total patients included in the study, the prevalence of OCI was 16.5%. Among these 15 total OCI patients, 1 was diagnosed by 14 ultracentrifuged serum results and 14 were diagnosed by peripheral blood mononuclear cell results. Compared to the non-OCI group, the OCI patients presented higher frequency of older age(P = 0.002), patients with CKD of mixed etiology(P = 0.019), and patients with markers of previous HBV infection(i.e.,combined positivity for anti-hepatitis B core protein antibody and anti-hepatitis B surface protein antibody)(P = 0.001).CONCLUSION Among predialysis patients, OCI involved the elderly, patients with CKD of mixed etiology, and patients with previous HBV infection.展开更多
文摘This work summarizes recently published information on the solar UV broadband irradiation of Pernambuco, Northeast of Brazil. We describe the spatial and temporal distribution of solar UV radiation and its relationship with climatic and geographical conditions. Statistical experimental correlation between solar total irradiation and UV broadband obtained for 03 locations was generalized by the use of Koppen-Geiger Climatic criterium, which was used for mapping the spatial/temporal distribution of broadband UV. The climatological solar radiations used in the correlations were obtained by modeling through satellite and previously verified with terrestrial data. We present one map with the location of the recording stations where the statistical correlations were measured, one annual and 12 monthly contour maps describing monthly daily solar UV radiation levels throughout the territory of Pernambuco. The solar UV irradiation (“broadband”) annual-average daily value in the State of Pernambuco varied from 226 to 268 Wh/m<sup>2</sup>. Seasonal variation of solar UV irradiation in the State of Pernambuco follows, in general and as expected, the climate, relief and seasons of the year. The highest value of monthly solar UV irradiation was observed in the central south region of the state, more precisely in Belém do São Francisco, Floresta, Ibimirim and Buíque in the month of December (summer), with 311.8 Wh/m<sup>2</sup>. The lower value was found in the south Agreste region, in Garanhuns and Caruaru, in the month of June (winter), with 162.2 Wh/m<sup>2</sup>.
文摘The aim of the present study was to evaluate different acaricide treatments for the control of Boophilus microplus on field-kept dairy cattle in the state of Pernambuco, Brazil. The first phase of the experiment consisted of collecting the ingurgitated female Boophilus microplus directly from the hosts for attainment of larvae. After the attainment of larvae, the artificial infestation procedure began on the cattle, with larvae ages ranging from 7 to 14 days. On Day 0, animals were separated into 12 groups to receive the corresponding treatment: Abamectin;Ivermectin;Ivermectin LA;Amitraz;Amitraz + Ivermectin;Amitraz + Ivermectin LA;Amitraz + Abamectin;Association (Cypermethrin + Chlorpyrifos + Citronella);Association + Ivermectin;Association + Ivermectin LA;Association + Abamectin;and Control. Subsequent evaluations were made on post-treatment days +7, +14, +21, +28, +35, +42, +49, +56 and +63. Analyzing the post-treatment effectiveness per day, indices revealed considerable variation ranging from 0% to 96.63%. Such indices demonstrate the significant reduction in the number of ticks on the animals in some groups, especially in the Abamectin group. The analysis of the results demonstrates that the use of different avermectines can assist in the development of Boophilus microplus control programs, thereby reducing the number of acaricide applications and production costs related to ticks.
基金Banco do Nordeste(BNB)for financing the studyBIOSOJA for donation inoculantCAPES and CNPq for the scholarship of masters and undergraduate.
文摘The greater use of crop fields by increasing plant population is an alternative to minimize losses to the farmers who use crop intercropped provides other benefits such as lower incidence of diseases and pests, and better exploitation and enrichment agroecosystem. The objective of this study was to evaluate the effect of the population of peanuts with and without inoculation and intercropping with forage palm on growth, productivity and biomass peanut. The treatments consisted of different populations of peanut (one, two and three rows of peanut row spacing of two meters of cactus pear), with and without inoculant, analyzed in subdivided plot scheme (3 × 2), totalizing six treatments in a randomized block design with four replications. The variables evaluated were plant height, number of branches, shoot dry mass and root nodule number, nodule dry weight, biomass productivity and legumes. The largest population of plants in number of lines of peanut intercropped with forage cactus promotes greater total biomass production and yield of beans and does not alter the height, branch number, dry mass of aerial part roots and nodules. The inoculant application did not result in to increased productivity biomass and growth of peanut plants, their use is not indicated. The planting of a row of peanuts between the rows of palm is not economically recommended.
文摘Isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to host.This study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains,as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in vitro.Goat yogurts with IG ingredients(IGI)stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation,as well as decreased the pH values and enhanced the organic acid production.Goat yogurts with IGI increased the population of Lactobacillus spp.and Bifidobacterium spp.during a 24-h in vitro colonic fermentation.A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation,being similar to the effect caused by fructooligosaccharides.Goat yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic fermentation.Goat yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.
文摘The Caatinga biome is an important ecosystem in the semi-arid region of Brazil.It has significantly degraded due to human activities and is currently a region undergoing desertification.Thus,monitoring the variation in the Caatinga biome has become essential for its sustainable development.However,traditional methods for estimating aboveground biomass(AGB)are time-consuming and destructive.Remote sensing,such as optical and radar imaging,can estimate and correlate with vegetation.Nevertheless,radar imaging is still a novelty to be applied in estimating the AGB of this biome,which is an area with little research.Therefore,this study aimed to use Sentinel-1 images to estimate the AGB of the Caatinga biome in Sergipe State(northeastern Brazil)and to verify its influencing factors.Nineteen sample plots(30 m×30 m)were selected,and the stems of individuals with a circumference at breast height(1.3 m above the ground)equal to or greater than 6.0 cm were measured,and the AGB through an allometric equation was estimated.The Sentinel-1 images from 3 different periods(green,intermediate,and dry periods)were used to consider the phenological conditions of the Caatinga biome.All the pre-processing and extraction of attributes(co-polarized VV(vertical transmit and vertical receive),cross-polarized VH(vertical transmit and horizontal receive),and band ratio VH/VV backscatter,radar vegetation index,dual polarization synthetic aperture radar(SAR)vegetation index(DPSVI),entropy(H),and alpha angle(α))were performed with Sentinel’s Application Platform.These attributes were used to estimate the AGB through simple and multiple linear regressions and evaluated by the coefficients of determination(R2),correlation(r),and root mean squared error(RMSE).The results showed that the attributes individually had little ability to estimate the AGB of the Caatinga biome in the three periods.Combined with multiple regression,we found that the intermediate period presented the equation with the best results among the observed and estimated variables(R^(2)=0.73;r=0.85;RMSE=8.33 Mg/hm^(2)),followed by the greenness period(R2=0.72;r=0.85;RMSE=8.40 Mg/hm^(2)).The attributes contributing to these equations were VH/VV,DPSVI,H,α,and co-polarized VV for the green period and cross-polarized VH for the intermediate period.The study showed that the Sentinel-1 images could be used to estimate the AGB of the Caatinga biome in the green and intermediate phenological periods since the SAR attributes highly correlated with the estimated variable(i.e.,AGB)through multiple linear equations.
文摘Enterococcus faecalis is a Gram-positive bacterium commonly found in the gastrointestinal tract that can cause serious infections. Many enterococci have broad resistance to antibiotics including penicillin, cephalosporins, aminoglycosides and glycopeptides. There are several adaptation mechanisms that bacteria can undergo to become more resistant, among them is the formation of biofilm. Several genes have been linked to the increase in the capacity of biofilm formation by bacteria such as gelE, esp and asa1. The aim of this research was to evaluate the biofilm formation of 12 E. faecalis isolates collected in hospitals and a standard strain, as well as to evaluate the hydrophobicity of its membrane and the presence of virulence genes. All the isolates formed biofilm and the characteristics of their membrane were variable. In addition, the presence of at least one virulence gene was found in all the 12 isolates, and none of the genes in the standard strain, indicating the acquisition of these genes in the hospital environment. With this, we can conclude that there is a close relationship between biofilm formation, acquisition of antibiotic resistance and the presence of virulence genes.
文摘Contextualization: Emptying the bladder is a challenging problem for the urological community. Intermittent catheterization is the most widely used method to restore bladder emptying mechanism. However, this procedure can have a negative impact on self-image and result in a decline in the quality of life of patients. In this context, the use of a bladder emptying device (SVCATH3D) proposes to be effective and have a positive impact on the quality of life of different patients. Objective: The objective of the study was to evaluate the functionality of a new device for both intermittent and controlled emptying of the bladder in both sexes and ages. Materials and methods: A randomized clinical trial was conducted with 251 patients, with different bladder problems, from March 2013 to January 2023. After randomization, the patients were divided into two groups: Group I (SVCATH3D) and Group II (Clean Intermittent Catheterization). The primary outcome was defined as the impact on quality of life. Data on episodes of urinary tract infection, adverse effects, number of diaper use and treatment costs were analyzed. Results: The apposition of the SVCATH3D was performed on an outpatient basis, with no complications during the procedures. The patients were followed up for 10 years. There was a significant improvement in quality of life when comparing the moments before and after the use of SVCATH3D (p Conclusion: The study using SVCATH3D showed promising results in relation to functionality, showing improvement in quality of life with a reduction in episodes of urinary infection and amount of diapers/day. This allows us to conclude that SVCATH3D can represent an important step in the treatment of patients suffering from various bladder problems both incontinence and affecting emptying mechanism.
文摘The use of medicinal plants as therapeutic and economic resources has been on the rise in recent years. In Brazil, however, increasing doubt is being cast on the quality of these products, owing to the prevalence of adulteration and fraud. Solar radiation can cause serious damage to human skin, as a result, mostly, of ultraviolet light, which is a cause of skin cancer. Photoprotective substances are capable of absorbing, reflecting, or refracting ultraviolet radiation and thus protecting skin from exposure to sunlight. The present study aimed to characterize samples and examine the phytochemical profile and photoprotective potential of bark and leaves of Erythrina velutina Willd. The samples underwent five extraction methods using 80% ethanol. The phenolic content was measured using spectrophotometry. Antioxidant activity was examined using the DPPH and the photoprotective properties of the plant extracts were assessed using the method developed by Mansur. There was a quantitative difference in some groups of metabolites, with higher levels of tannin in the bark and of flavonoids in the leaves. The latter showed greater DPPH free radical scavenging capacity than the bark, although higher levels of SPF were obtained from the bark, with no statistically significant differences between methods. The results indicate that Erythrina velutina Willd. has potential as a photoprotector.
基金supported by the Canadian Institutes of Health Research(CIHR),Grant No.AAM 108751.
文摘BACKGROUND The association of cardiovascular risk burden with disability is unclear.We examined the association between trajectories of the Framingham general cardiovascular disease risk score(FGCRS)with the trajectories of limitations of physical function in older adults.METHODS A total of 1219 participants with no disabilities from the International Mobility in Aging Study(IMIAS)study who had up to three repeated measures of FGCRS between 2012–2016 and without a history of stroke or coronary heart disease at baseline and follow-up were included.FGCRS at baseline was assessed and categorized into tertiles.Physical function was evaluated with the Short Physical Performance Battery(SPPB).The data were analyzed using linear mixed-effects models.RESULTS At baseline,FGCRS ranged between 3–94(mean score:24±15.8),participants were 32(2.6%),502(41.2%)and 685(56.2%)in lowest,middle,and highest tertiles,respectively.In the trajectories of limitations of physical function,the lowest FGCRS had no differences,while the middle and highest had a decrease in physical performance between 2012–2014(P=0.0001).Age,being female,living in Andes Mountains,having middle and highest FGCRS,higher alcohol consumption,being obese,lack of exercise and cognitive impairment increase the probability of disability(P<0.05).Alternatively,living in more developed regions and having a higher educational level reduced the probability of disability during the follow-up time(P<0.05).CONCLUSIONS Higher cardiovascular risk burden is associated with decreased physical performance,especially in gait.Results suggest SPPB may provide a measure of cardiovascular health in older adults.
文摘Since the first reports of coronavirus disease 2019(COVID-19)cases in December 2019 in China,numerous papers have been published describing a high frequency of liver injury associated with severe acute respiratory syndrome coronavirus 2 infection,many of them proposing a link between these findings and patient outcomes.Increases in serum aminotransferase levels(ranging from 16%to 62%)and bilirubin levels(ranging from 5%to 21%)have been reported and seem to be more often observed in patients with severe forms of COVID-19.Although absolute changes in these parameters are frequently seen,other variables,such as the ratio above the upper limit of normal,the onset of liver injury as a complication in severe cases and histopathological findings,reinforce that liver changes are of dubious clinical relevance in the course of this disease.Other factors must also be considered in these analyses,such as the repercussions of hemodynamic changes,the presence of thrombotic events,and,mainly,the possible drug-induced liver injury with the current,yet off-label,treatment.This paper aimed to analyze the currently available data on liver injury in patients with COVID-19.
基金the Fundacao de Apoio à Pesquisa do Estado da Paraíba (FAPESQ, Brazil)Coordenacao de Aperfeicoamento de Pessoal de Nível Superior (CAPES) for the scholarships awarded to Lacerda DCthe research productivity fellowship granted by the Brazilian National Council for Scientifc and Technological Development (CNPq) to de Brito Alves, JL
文摘Oxidative stress,inflammation,and gut microbiota impairments have been implicated in the development and maintenance of diabetes mellitus.Strategies capable of recovering the community of commensal gut microbiota and controlling diabetes mellitus have increased in recent years.Some lactobacilli strains have an antioxidant and anti-inflammatory system capable of protecting against oxidative stress,inflammation,and diabetes mellitus.Experimental studies and some clinical trials have demonstrated that Limosilactobacillus fermentum strains can beneficially modulate the host antioxidant and anti-inflammatory system,resulting in the amelioration of glucose homeostasis in diabetic conditions.This review presents and discusses the currently available studies on the identification of Limosilactobacillus fermentum strains with anti-diabetic properties,their sources,range of dosage,and the intervention time in experiments with animals and clinical trials.This review strives to serve as a relevant and well-cataloged reference of Limosilactobacillus fermentum strains capable of inducing anti-diabetic effects and promoting health benefits.
基金Supported by the National Research Council(CNPq)Foundation for Science and Technology of the State of Pernambuco(FACEPE)Personnel Improvement Coordination-CAPES/PROCAD/NF/no 1415/2007,Brazil
文摘Objective: To explore the effects of aqueous extract from Phthirusa pyrifolia leaves(67 mg/kg body weight for 12 days) on the reproductive function of male Wistar rats through oral administration.Methods: Animals(n = 30), aged 13 weeks and weighing(378.5 ± 5.0) g, were housed in a vivarium under controlled environmental conditions [photoperiod of 12 h light/dark,temperature of(23 ± 1)C] and were fed standard rations ad libitum. The experiment ran for 12 days, wherein animals were divided into three groups: negative control(n = 6)received water, positive control(n = 12) with finasteride at a concentration of 1.0 mg/kg;and a test group(n = 12) submitted to aqueous extract. At the end of the experiment, the animals were sacrificed and submitted to analyses.Results: The morphological results of the testes showed that the aqueous extract induced significant changes in the diameter and cross-sectional area of the seminiferous tubules as well as the thickness of the seminiferous epithelium. Furthermore, the extract was able to abruptly decrease testosterone concentrations by about 81.88% in the treated group when compared with the negative control,(47.0 ± 4.8) ng/d L and(255.0 ± 2.0) ng/d L,respectively, and 76.8%,(211.0 ± 8.7) ng/d L, when compared with finasteride. However,the extract causes neither liver damage nor impairment of renal function.Conclusions: These results suggest that the high amounts of flavonoids shown to be in the extract may be responsible for its hepato-protective effects and suggest a possible decrease in the libido and reproduction of rats.
文摘The main objective of this paper was to evaluate the daily actual evapotranspiration (ET) accuracy obtained by remote sensing algorithms when compared with Bowen ratio measurements performed in the cotton fields. The experiment was conducted in a cotton experimental field of EMBRAPA located in Ceará State, Brazil. Seven TM Landsat-5 images acquired in 2005 and 2008 were used to perform SEBAL and SSEB algorithms. The comparison between the estimated values by remoting sensing algorithms and the measured values in situ showed an acceptable accuracy. Besides, SSEB algorithm showed to be an important tool for ET analysis in the semi-arid regions, due to the fact that it does not need the meteorological data to solve the energy balance, but only the average temperature of the “hot” and “cold” pixels. Additionally, SSEB presents simpler processing than SEBAL algorithm that needs to solve an iterative process to obtain the sensible heat flux values.
文摘Objective: The study aims to evaluate the use of a teaching method proposed by Paulo Freire, Culture Circles, in the education of teenagers multipliers on leprosy awareness. Methods: It is an action-research study with a qualitative approach developed in a public school in Pernambuco, Brazil. Five Culture Circles were conducted involving the participation of 26 teenagers. The followings were used as data collection tools: observation, field notes, photography and filming. Results: The educational intervention on health addressed the following topics: 1) Definition and transmission of leprosy;2) Characteristics and diagnosis of leprosy;3) Treatment of leprosy;4) Aesthetics, prejudice and mental health related to leprosy;and 5) Planning of educational activities for teenagers as health multipliers on leprosy awareness. The educational action on health provided this age group to perceive themselves, act as political subjects in the development of Culture Circles, and act as protagonists in the dissemination of knowledge on leprosy. Conclusions: This study highlights that the application of active methodologies, such as Culture Circles, is able to encourage the engagement of young people in community empowerment and bring together health professionals and the school community in an intersectoral work in order to develop action strategies involving the promotion of health in the context of neglected diseases such as leprosy.
文摘Sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architecture and biomass. The expectation is also an increase in sugarcane biomass. The study hypothesized that high N rates applied to sugarcane fields increases root growth and N stored in roots, promoting higher biomass and N accumulated in shoots. Two experiments were set up in Southeastern Brazil, on a Typic Kandiudox (TK) and Rhodic Eutrudox (RE). Four treatments were studied 1) N application in the plant-cane (0 and 120 kg·ha-1 N) and 2) N application in the ratoon (0 and 150 kg·ha-1 N). The shoot biomass and the root density (by the core method up to 0.6 m) were evaluated over the first ratoon crop cycle, and the N content in those compartments was also examined. There was no carry over effect on N applied at planting in root and shoot biomass in the ratoon crop cycle. At the RE site, the ratoon N fertilization increased root density in the superficial soil layer (0 - 0.2 m) and close to the plants (<0.3 m). The effect of N addition on root biomass, and biomass and N accumulated in shoot was limited in both sites. Increasing N rates in unburned sugarcane fields do not consistently increases root and shoot biomass under Brazilian field conditions.
文摘In this paper, data analysis and modeling of gamma ray tomography taken into account spatial resolution and source of errors and the attenuation coefficient measurement in row data from tomography process are presented. The results showed that this method is simple, effective and should be prior to any data treatment for opaque vessel reactor and by reconstruction algorithm in process imaging.
文摘The objective of this work is to assess the impacts of IPCC AR5 climate change scenarios on water resources and hydrological processes across the entire Brazilian territory. Hydrological simulations are carried out in total drainage area of about 11,535,645 km<sup>2</sup> and average stream flow of about 272,460 m<sup>3</sup>/s. The study area consists of different climates and land covers such as the Amazon Forest, Northeast Semiarid, Brazilian Savannah, Pantanal wetlands and temperate climate in the South. The atmospheric forcing to drive the large-scale hydrological model MGB-IPH is derived from the downscaling of two global climate models, HadGEM2-ES and MIROC5, by the Eta Regional Climate Model, at 20 km resolution. The Eta model provided the downscaling of the baseline (1961-1990) and three time-slices (2011-2040, 2041-2070 and 2071-2099). These projections adopted two emission scenarios, the RCP 4.5 and RCP 8.5. The change in the average and extremes of precipitation, evapotranspiration, rates of river discharge and soil moisture were assessed. The simulations showed the response of the hydrographic regions due to change of precipitation and potential evapotranspiration in the scenarios. Water availability decreases in almost the entire study area (exception for the South) and the major basins for hydroelectric power generation are affected. The Northwest, Amazon and a small area along the Northeast Atlantic coast exhibited intensification of the extremes discharges, where the anomaly is positive for high-flow (Q<sub>10</sub>) and negative for low-flow (Q<sub>95</sub>). The results highlight the most climatic sensitive regions in Brazil in terms of hydrological variables and water resources.
文摘Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal cells.Methods:The saline extract was prepared,with NaCl(0.15 M),by constant stirring of the dried and pulverized leaves,followed by volume reduction by lyophilization.The extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was performed.The antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice splenocytes.Results:Fifteen compounds were identified belonging to two main classes terpenoids and phenolics.The extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of flavonoid.Moreover,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these cells.Conclusions:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.
基金Supported by the National Council for Scientific and Technological Development(CNPq),No.429736-2016/9
文摘BACKGROUND Occult hepatitis C virus(HCV) infection(OCI) may be associated with extrahepatic diseases and it is known that the patients with chronic kidney disease(CKD) who are on hemodialysis(HD) present a higher prevalence of this type of infection than the general population, with a worse clinical outcome.However, there are no data in the literature to assess the presence of OCI in patients prior to the initiation of renal replacement therapy(RRT). Therefore, this study aimed to evaluate the occurrence and epidemiological aspects of OCI in patients with Predialysis CKD. We hypothesize that this infection could occur before RRT initiation.AIM To research the status in predialysis patients when HD patients have high prevalence of OCI.METHODS A cross-sectional study was conducted between 2015 and 2017. Adults with creatinine clearance < 60 mL/min·1.73 m^2(predialysis patients) were recruited to the study. Pregnant and postpartum women, patients with glomerulopathies,and patients showing positivity for serological markers of hepatitis B virus(HBV), HCV or human immunodeficiency virus infection were excluded. Patients were diagnosed with OCI according to test results of anti-HCV antibody negativity and HCV RNA positivity in either ultracentrifuged serum or, if serumnegative, in peripheral blood mononuclear cells.RESULTS Among the 91 total patients included in the study, the prevalence of OCI was 16.5%. Among these 15 total OCI patients, 1 was diagnosed by 14 ultracentrifuged serum results and 14 were diagnosed by peripheral blood mononuclear cell results. Compared to the non-OCI group, the OCI patients presented higher frequency of older age(P = 0.002), patients with CKD of mixed etiology(P = 0.019), and patients with markers of previous HBV infection(i.e.,combined positivity for anti-hepatitis B core protein antibody and anti-hepatitis B surface protein antibody)(P = 0.001).CONCLUSION Among predialysis patients, OCI involved the elderly, patients with CKD of mixed etiology, and patients with previous HBV infection.