Agolin Ruminant®, an Essential Oil Blend, Increases Energy-Corrected Milk and Feed Efficiency in a High Component Dairy Herd

Agolin® Ruminant (Agolin) is a blend of essential oils developed to improve feed efficiency while at the same time reducing rumen enteric methane production. Studies have shown that the product improves lactational performance, but the range of results has been varied. This experiment evaluated the effects of the feed additive Agolinon milk production in a high-component (fat and protein) dairy herd when provided for an extended time. The experiment was conducted at a large commercial dairy in the Pacific Northwest, USA. Eight pens of cows (350 to 500 cows/pen) were blocked by production, and pens within blocks were randomly assigned to treatment. All pens received a common total mixed ration. Treatment consisted of providing 1 g/cow/day of Agolin to the test group. A 4-week adaptation period was followed by an 11-week study period. Milk production and milk composition were determined by cow by averaging daily performance for the one-week period before the start of the trial (covariate period) and the last week of each of the two test periods. Feed intakes were determined by pen at the same time. Milk yield was 1.11 kg/cow/day greater (P < 0.001) at the midpoint and 1.48 kg/cow/day greater (P < 0.001) at the end of the test period for cows receiving the feed additive. Similarly, protein yields were 0.03 (P < 0.001) and 0.07 kg/cow/day greater (P < 0.001) at the midpoint and end of the feeding period. Milk fat yield was not different (P = 0.854) between treatment groups at the midpoint of the trial but increased with treatment (P = 0.002) by the end of the trial. Energy-corrected milk/feed ratio, based on pen parameters, was significantly altered by treatment at the final test period (1.48 vs. 1.64 kg/kg for control and test treatments, respectively).

Evaluation of Agolin^®, an Essential Oil Blend, as a Feed Additive for High Producing Cows

There is a growing demand for feed additives that can not only reduce dairy enteric methane emissions but also increase milk production and feed efficiency. Just one product is currently commercially available which accomplishes both of these goals. The purpose of this study was to confirm the performance benefits of the product (Agolin?, Agolin SA, Biere, Switzerland) in high producing mid-lactation dairy cows under United States feeding conditions. Four matched pens of approximately 150 mid-lactation cows/pen and averaging over 50 kg of milk/cow/day were enrolled in a side-by-side study. All pens received a common total mixed diet ad libitum, and the essential oil blend was administered via a concentrated farm pack to provide 1 g/cow/day to cows in the 2 test pens. Milk weights were determined, and samples were collected for compositional analysis over the last 2 days of the pretrial (May 11 and 12, 2020) and end of the trial (July 18 and 19, 2020) periods. Dry matter intake was measured by pen daily for the last 10 days of each feeding period. Milk fat and milk protein yields were greater (P < 0.05) for cows receiving added Agolin. There was a tendency (P = 0.06) for energy corrected milk/dry matter intake to be greater for cows receiving the Agolin (1.88) relative to the control diet (1.76). The trial showed that Agolin assisted in improving production parameters of economic importance to dairy producers.

Evaluation of Agolin® Ruminant, an Essential Oil Blend, as a Feed Additive for Cows at Two Levels of Production

Agolin® Ruminant (Agolin) is a commercially available blend of essential oils which has been demonstrated to reduce greenhouse gas emissions in dairy cows and improve energy corrected milk and feed efficiency. Further trials are needed with large numbers of cows to confirm the magnitude of effect of this feed additive on milk production under differing feeding conditions and stages of lactation. Information that can be quantified from newer on-farm systems of measurement is likewise needed. This study was conducted to determine the effects of this additive on high producing (48 kg of milk/day average) just past peak lactation and medium producing dairy cows (43 kg of milk/day average) further along in lactation under commercial conditions that would typically occur in the Pacific Northwest USA. Four pens of approximately 400 Holstein cows/pen (two high producing pens and two medium producing pens) were available for this side-by side study. Performance results were determined using data derived from a subscription standardized monitoring system (High Desert Dairy Laboratory, Inc, Nampa, ID, USA) that provided results for milk yield, fat percentage and protein percentage. As well daily in-stall electronic monitoring of milk only was available. All pens received a common total mixed ration typical of rations fed in the Pacific Northwest, USA. The Agolin feed additive was dispensed through the bulk mineral supply with control cows receiving the normal minerals and the treatment cows receiving the normal minerals plus Agolin. The trial began on August 24, 2021, with treatment cows provided with 1 g/head/day for the duration of the 8-week long study. Dry matter intakes were determined daily for the week before the trial was conducted and the last week of the trial. Treatment results were compared using a general linear model that considered pretrial milk, fat yield and protein yield, days in milk and lactation number. There were 678 high producing cows available for the duration of the study. Based on the standardized testing results, milk yield was greater (1.12 kg/cow/day;P < 0.05) for the cows in the treatment group. There was a tendency for fat corrected milk (FCM) and energy corrected milk (ECM) to be greater for the cows receiving the Agolin feed additive (0.96 and 0.86 kg/cow/day for FCM and ECM respectively, P < 0.10). There were 646 medium producing cows that participated in the trial. There was no treatment effect upon milk production (P = 0.27). There was a significant (P < 0.05) increase in milk fat yield and no change (P = 0.33) in protein yield for the treatment cows in this test group, resulting in greater FCM and ECM (1.12 and 0.95 kg/cow/day;P < 0.05). Daily milk monitoring resulted in a reduced decline in milk yield from the week before to the final week of the trial revealing greater persistency of milk for Agolin-fed cows in both high and medium production pens (1.58 and 2.13 kg/cow/day;P < 0.01). Likewise, overall feed efficiency was improved by 5.3% (P < 0.05) with the test product.

Effect of Stabilized Fish Oil Source on Sperm Quality and Production of Boars

Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of the fatty acid source, level of inclusion and breed of boars tested. Three groups of 87 boars each, from two genetic lines (PIC 337 and PIC 800), were assigned to treatment based on age, mean sperm production (previous 12 weeks), and body condition score. All boars received a corn-soybean meal diet with a commercial fish oil supplement providing 1.83 g/boar/day of docosahexaenoic acid (DHA) as a preconditioning diet. On 10-Aug., 2020, the DHA source was changed to a stabilized starch imbedded source of refined fish oil (Salmate®), providing 1.83 g/b/d for the test diet. Two additional levels providing 2.38 and 2.94 g/b/d of DHA were fed for a 9 week pretreatment period and during the test period. Salmate® fed at 2.38 g/b/d of DHA resulted in a reduction in the number of rejected ejaculates (P < 0.045) by 7.5% and 6.4% compared to the lowest and highest inclusion rates, respectively. There were no treatments by genetic line interactions. A retrospective study of semen production and quality of 77 boars on the Salmate® diet containing 1.83 g/b/d DHA was done to compare to the original source of DHA at the same inclusion level. There were no differences in semen quality parameters between the 2 lipid sources. Ejaculate volume increased from 177.9 ml to 233.4 ml (P < 0.001) and total sperm cells per ejaculate increased from 69.7 × 109 to 82.0 × 109 (P < 0.001) due to substitution of Salmate®. Adding Salmate® at 2.38 g/b/d resulted in a lower number of rejected ejaculates per boar by 7.5% and 6.4% vs. 1.83 and 2.94 g/b/d, respectively, and boars fed Salmate® at 1.83 g/b/d produced 17% more doses than the competing product.