Effect of early stepwise cardiopulmonary rehabilitation on function and quality of life in sepsis patients

BACKGROUND Sepsis,as a non-limiting host infection disease,can be accompanied by serious complications such as organ failure,which seriously threatens patient quality of life.AIM To investigate the effect of early stepwise cardiopulmonary rehabilitation on cardiopulmonary function and quality of life in patients evacuated from mechanical ventilation with sepsis.METHODS A total of 80 patients with sepsis who were hospitalized in our hospital from January 2021 to January 2022 were selected and divided into the observation group(n=40)and the control group(n=40)according to the random number table method.The observation group was treated with early stepwise cardiopulmonary rehabilitation,and the control group was treated with a conventional treatment regimen.Cardiac function indexes(central venous pressure,cardiac troponin I,B-type brain natriuretic peptide),lung function indicators(diaphragmatic mobility,changes in central venous oxygen saturation,oxygenation index),and quality of life(Quality of Life Evaluation Scale)were compared between the two groups after treatment.RESULTS After treatment,the central venous pressure,diaphragm mobility,central venous oxygen saturation,oxygenation index,and Quality of Life Evaluation Scale scores in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.05).The observation group was less than that of the control group for other parameters,and the differences were statistically significant(P<0.05).CONCLUSION Early stepwise cardiopulmonary rehabilitation can effectively enhance cardiac and pulmonary function and improve the quality of life in patients evacuated from mechanical ventilation with sepsis.

Effects of hypoxia inducible factor-1alpha siRNA on theinvasion of human Hela cells and expression of relatedproteins

The effects of hypoxia on the invasion and the related protein expression of Hela cells and the role of hypoxia inducible factor-1α(HIF-1α)were investigated.The Hela cells were divided into three groups,namely,H0(non-transfected Hela cells),H1(pGenesil-1 empty plasmid-transfected Hela cells),and H2(HIF-1α-shRNA plasmid-transfected Hela cells),and were cultured under hypoxia(1%O2)and normoxia for 48 h.The expression of HIF-1α,E-cadherin,β-catenin,and actin was detected using Western blot.The scratch test and the invasion assay were applied to examine the invasion in each group.The expression of HIF-1α,E-cadherin,andβ-catenin in tumor grafts was assayed immunohistochemically.Western blot results revealed that the bands of HIF-1α,E-cadherin,β-catenin,and actin proteins were detected in the H0 and H1 groups under hypoxia for 48 h.The expression of E-cadherin,β-catenin,and actin was detected in the H2 group under hypoxia for 48 h,and normoxia.In the H0,H1,and H2 groups under normoxia,and the H2 group under hypoxia for 48 h,no expression of HIF-1αwas detectable.The scratch test showed that the invasive ability in the H2 group was significantly alleviated.Immunohistochemi-cally,it was found that there was a significant difference in the expression of HIF-1α,E-cadherin,andβ-catenin between the H1 and H2 groups(P<0.05),but the difference was not significant between the H0 and H1 groups.It was concluded that the effects of hypoxia on the invasion of human cervical cancer Hela cells and the expression of related proteins(E-cadherin,β-catenin,and actin)depend on HIF-1α.