Glass-ceramics are often used as sealants in solid oxide fuel cells (SOFC). But interfacing components, such as ferritic stainless steel and YSZ electrolyte, may vary in their requirements regarding sealing properties...Glass-ceramics are often used as sealants in solid oxide fuel cells (SOFC). But interfacing components, such as ferritic stainless steel and YSZ electrolyte, may vary in their requirements regarding sealing properties, especially in terms of thermal expansion. A bilayered glass-ceramic system was developed to overcome the mismatch in coefficients of thermal expansion (CTE) between ferritic steel and YSZ. Therefore, two different glass-ceramics with slightly different CTEs were developed, one with good bonding characteristics to the ferritic steel and the other to the YSZ electrolyte. Steel and electrolyte components were coated with a layer of their corresponding glass sealant paste and heated up to form a sandwich sample. During the heat treatment of the sealing process, the glasses are crystallized into glass-ceramics. The resulting interface between the two glass-ceramics is of special interest. Cross-sections of the sandwich samples were cut, polished and investigated using SEM. The glass-ceramics show continuous, gap-free layers and excellent bonding to both steel and YSZ. Energy release rates are measured for single and bilayered glass sealants by mechanical testing. The designed bilayered glass-ceramics fulfill the special requirements of ferritic steel and YSZ. They show excellent potential to become a new outstanding sealant for SOFCs.展开更多
Bone Morphogenetic proteins(BMPs)like BMP2 and BMP7 have shown great potential in the treatment of severe bone defects.In recent in vitro studies,BMP9 revealed the highest osteogenic potential compared to other BMPs,p...Bone Morphogenetic proteins(BMPs)like BMP2 and BMP7 have shown great potential in the treatment of severe bone defects.In recent in vitro studies,BMP9 revealed the highest osteogenic potential compared to other BMPs,possibly due to its unique signaling pathways that differs from other osteogenic BMPs.However,in vivo the bone forming capacity of BMP9-adsorbed scaffolds is not superior to BMP2 or BMP7.In silico analysis of the BMP9 protein sequence revealed that BMP9,in contrast to other osteogenic BMPs such as BMP2,completely lacks so-called heparin binding motifs that enable extracellular matrix(ECM)interactions which in general might be essential for the BMPs’osteogenic function.Therefore,we genetically engineered a new BMP9 variant by adding BMP2-derived heparin binding motifs to the N-terminal segment of BMP9′s mature part.The resulting protein(BMP9 HB)showed higher heparin binding affinity than BMP2,similar osteogenic activity in vitro and comparable binding affinities to BMPR-II and ALK1 compared to BMP9.However,remarkable differences were observed when BMP9 HB was adsorbed to collagen scaffolds and implanted subcutaneously in the dorsum of rats,showing a consistent and significant increase in bone volume and density compared to BMP2 and BMP9.Even at 10-fold lower BMP9 HB doses bone tissue formation was observed.This innovative approach of significantly enhancing the osteogenic properties of BMP9 simply by addition of ECM binding motifs,could constitute a valuable replacement to the commonly used BMPs.The possibility to use lower protein doses demonstrates BMP9 HB’s high translational potential.展开更多
文摘Glass-ceramics are often used as sealants in solid oxide fuel cells (SOFC). But interfacing components, such as ferritic stainless steel and YSZ electrolyte, may vary in their requirements regarding sealing properties, especially in terms of thermal expansion. A bilayered glass-ceramic system was developed to overcome the mismatch in coefficients of thermal expansion (CTE) between ferritic steel and YSZ. Therefore, two different glass-ceramics with slightly different CTEs were developed, one with good bonding characteristics to the ferritic steel and the other to the YSZ electrolyte. Steel and electrolyte components were coated with a layer of their corresponding glass sealant paste and heated up to form a sandwich sample. During the heat treatment of the sealing process, the glasses are crystallized into glass-ceramics. The resulting interface between the two glass-ceramics is of special interest. Cross-sections of the sandwich samples were cut, polished and investigated using SEM. The glass-ceramics show continuous, gap-free layers and excellent bonding to both steel and YSZ. Energy release rates are measured for single and bilayered glass sealants by mechanical testing. The designed bilayered glass-ceramics fulfill the special requirements of ferritic steel and YSZ. They show excellent potential to become a new outstanding sealant for SOFCs.
文摘Bone Morphogenetic proteins(BMPs)like BMP2 and BMP7 have shown great potential in the treatment of severe bone defects.In recent in vitro studies,BMP9 revealed the highest osteogenic potential compared to other BMPs,possibly due to its unique signaling pathways that differs from other osteogenic BMPs.However,in vivo the bone forming capacity of BMP9-adsorbed scaffolds is not superior to BMP2 or BMP7.In silico analysis of the BMP9 protein sequence revealed that BMP9,in contrast to other osteogenic BMPs such as BMP2,completely lacks so-called heparin binding motifs that enable extracellular matrix(ECM)interactions which in general might be essential for the BMPs’osteogenic function.Therefore,we genetically engineered a new BMP9 variant by adding BMP2-derived heparin binding motifs to the N-terminal segment of BMP9′s mature part.The resulting protein(BMP9 HB)showed higher heparin binding affinity than BMP2,similar osteogenic activity in vitro and comparable binding affinities to BMPR-II and ALK1 compared to BMP9.However,remarkable differences were observed when BMP9 HB was adsorbed to collagen scaffolds and implanted subcutaneously in the dorsum of rats,showing a consistent and significant increase in bone volume and density compared to BMP2 and BMP9.Even at 10-fold lower BMP9 HB doses bone tissue formation was observed.This innovative approach of significantly enhancing the osteogenic properties of BMP9 simply by addition of ECM binding motifs,could constitute a valuable replacement to the commonly used BMPs.The possibility to use lower protein doses demonstrates BMP9 HB’s high translational potential.