Traction force microscopy(TFM)is one of the most successful and broadly-used force probing technologies to quantify the mechanical forces in living cells.The displacement recovery of the fluorescent beads within the g...Traction force microscopy(TFM)is one of the most successful and broadly-used force probing technologies to quantify the mechanical forces in living cells.The displacement recovery of the fluorescent beads within the gel substrate,which serve as the fiducial markers,is one of the key processes.The traditional methods of extracting beads displacements,such as PTV,PIV,and DIC,persistently suffer from mismatching and loss of high-frequency information while dealing with the complex deformation around the focal adhesions.However,this information is crucial for the further analysis since the cells mainly transmit the force to the extracellular surroundings through focal adhesions.In this paper,we introduced convolutional neural network(CNN)to solve the problem.We have generated the fluorescent images of the non-deformable fluorescent beads and the displacement fields with different spatial complexity to form the training dataset.Considering the special image feature of the fluorescent images and the deformation with high complexity,we have designed a customized network architecture called U-DICNet for the feature extraction and displacement estimation.The numerical simulation and real experiment show that U-DICNet outperforms the traditional methods(PTV,PIV,and DIC).Particularly,the proposed U-DICNet obtains a more reliable result for the analysis of the local complex deformation around the focal adhesions.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11872354,11627803,and 12102423)the National Science and Technology Major Project(Grant No.J2019-V-0006-0100)。
文摘Traction force microscopy(TFM)is one of the most successful and broadly-used force probing technologies to quantify the mechanical forces in living cells.The displacement recovery of the fluorescent beads within the gel substrate,which serve as the fiducial markers,is one of the key processes.The traditional methods of extracting beads displacements,such as PTV,PIV,and DIC,persistently suffer from mismatching and loss of high-frequency information while dealing with the complex deformation around the focal adhesions.However,this information is crucial for the further analysis since the cells mainly transmit the force to the extracellular surroundings through focal adhesions.In this paper,we introduced convolutional neural network(CNN)to solve the problem.We have generated the fluorescent images of the non-deformable fluorescent beads and the displacement fields with different spatial complexity to form the training dataset.Considering the special image feature of the fluorescent images and the deformation with high complexity,we have designed a customized network architecture called U-DICNet for the feature extraction and displacement estimation.The numerical simulation and real experiment show that U-DICNet outperforms the traditional methods(PTV,PIV,and DIC).Particularly,the proposed U-DICNet obtains a more reliable result for the analysis of the local complex deformation around the focal adhesions.
