Articular cartilage plays an important role in weight-bearing and movement.However,it is also more easily damaged in intra-articular fractures.As the cartilage has no vessel and nerve,so it is hardly to repair itself ...Articular cartilage plays an important role in weight-bearing and movement.However,it is also more easily damaged in intra-articular fractures.As the cartilage has no vessel and nerve,so it is hardly to repair itself once damaged seriously.Tissue engineering is a potential way to cure the cartilage damage because it combines scaffold and cells.Gels展开更多
Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic properties.Herein,we...Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic properties.Herein,we report a green,facile,efficient,fast strategy to access high-performance nanozymes via supercritical CO_(2)fluid technology-fabricated polymer nanoreactor of poly-(methyl vinyl ether-co-maleic anhydride)(PVM/MA)coated Co(NO_(3))_(2)into C-doped Co_(3)O_(4)(C-Co_(3)O_(4))nanozyme by a onestep calcination process.Converting PVM/MA to C doping into Co_(3)O_(4)shortens the entire lattice constant of the crystal structure,and the overall valence band energy level below the Fermi level shifts toward the lower energy direction.The as-prepared CCo_(3)O_(4)demonstrated significant peroxidase-like catalytic activity,significantly greater than the undoped Co_(3)O_(4)nanoparticle nanozyme.The following density functional theory(DFT)calculations revealed that the doped nano-enzyme catalytic site displayed a unique electronic structure,altering the material surface with more electrons to fill the anti-bond of the two molecular orbitals,significantly improving the peroxidase-like enzyme catalytic and glucose sensor performance.The resultant enzymatic glucose sensing in a linear range of 0.1–0.6 mM with a detection limit of 3.86μM is in line with standard Michaelis–Menten theory.Collectively,this work demonstrates that converting polymers into nanozymes of C-doped form by supercritical CO_(2)fluid technology in a step is an effective strategy for constructing high-performance glucose sensor nanozymes.This cost-effective,reliable,precise system offers the potential for rapid analyte detection,facilitating its application in a variety of fields.展开更多
Recently tremendous progress has been evidenced by the advancements in developing innovative three-dimensional(3 D)scaffolds using various techniques for addressing the autogenous grafting of bone. In this work, we de...Recently tremendous progress has been evidenced by the advancements in developing innovative three-dimensional(3 D)scaffolds using various techniques for addressing the autogenous grafting of bone. In this work, we demonstrated the fabrication of porous polycaprolactone(PCL) scaffolds for osteogenic differentiation based on supercritical fluid-assisted hybrid processes of phase inversion and foaming. This eco-friendly process resulted in the highly porous biomimetic scaffolds with open and interconnected architectures. Initially, a 2^3 factorial experiment was designed for investigating the relative significance of various processing parameters and achieving better control over the porosity as well as the compressive mechanical properties of the scaffold. Then, single factor experiment was carried out to understand the effects of various processing parameters on the morphology of scaffolds. On the other hand, we encapsulated a growth factor, i.e., bone morphogenic protein-2(BMP-2), as a model protein in these porous scaffolds for evaluating their osteogenic differentiation. In vitro investigations of growth factor loaded PCL scaffolds using bone marrow stromal cells(BMSCs) have shown that these growth factor-encumbered scaffolds were capable of differentiating the cells over the control experiments. Furthermore, the osteogenic differentiation was confirmed by measuring the cell proliferation, and alkaline phosphatase(ALP) activity, which were significantly higher demonstrating the active bone growth. Together, these results have suggested that the fabrication of growth factor-loaded porous scaffolds prepared by the eco-friendly hybrid processing efficiently promoted the osteogenic differentiation and may have a significant potential in bone tissue engineering.展开更多
Recently,hydrogels have gained enormous interest in three-dimensional(3D)bioprinting toward developing functional substitutes for tissue remolding.However,it is highly challenging to transmit electrical signals to cel...Recently,hydrogels have gained enormous interest in three-dimensional(3D)bioprinting toward developing functional substitutes for tissue remolding.However,it is highly challenging to transmit electrical signals to cells due to the limited electrical conductivity of the bioprinted hydrogels.Herein,we demonstrate the 3D bioprinting-assisted fabrication of a conductive hydrogel scaffold based on poly-3,4-ethylene dioxythiophene(PEDOT)nanoparticles(NPs)deposited in gelatin methacryloyl(GelMA)for enhanced myogenic differentiation of mouse myoblasts(C2C12 cells).Initially,PEDOT NPs are dispersed in the hydrogel uniformly to enhance the conductive property of the hydrogel scaffold.Notably,the incorporated PEDOT NPs showed minimal influence on the printing ability of GelMA.Then,C2C12 cells are successfully encapsulated within GelMA/PEDOT conductive hydrogels using 3D extrusion bioprinting.Furthermore,the proliferation,migration and differentiation efficacies of C2C12 cells in the highly conductive GelMA/PEDOT composite scaffolds are demonstrated using various in vitro investigations of live/dead staining,F-actin staining,desmin and myogenin immunofluorescence staining.Finally,the effects of electrical signals on the stimulation of the scaffolds are investigated toward the myogenic differentiation of C2C12 cells and the formation of myotubes in vitro.Collectively,our findings demonstrate that the fabrication of the conductive hydrogels provides a feasible approach for the encapsulation of cells and the regeneration of the muscle tissue.展开更多
Despite the success,it is highly challenging to battle against pathogenic biofilms-based chronic bacterial infections by conventional antibiotic therapy.Herein,we report a near-infrared(NIR)/acid-induced nanoplatform ...Despite the success,it is highly challenging to battle against pathogenic biofilms-based chronic bacterial infections by conventional antibiotic therapy.Herein,we report a near-infrared(NIR)/acid-induced nanoplatform based on chitosan(CS)-coated indocyanine green(ICG,photosensitizer)/luteolin(LUT,a natural quorum sensing inhibitor)nanocomposites(ICG/LUT-CS)as antibacterial and antibiofilm agents for skin wound healing.Initially,the ICG/LUT nanoplatforms are prepared by the supercritical antisolvent technology and coated with the CS layer.The obtained ICG/LUT-CS with ultra-high encapsulation efficiency exhibited more favorable photothermal conversion effects and improved NIR laser/acid dual-induced drug release behavior than individual modalities,achieving exceptional bacteria-killing and biofilm elimination effects.Moreover,the ICG/LUT-CS realized the synergetic effects of chemotherapy and photothermal therapy outcomes for wound healing.Together,our findings provided an appealing strategy for the rapid preparation and future translational application of ICG/LUT-CS as an ideal agent for fighting against biofilm infections.展开更多
We describe a route to the preparation of(metal yolk)/(porous ceria shell)nanostructures through the heterogeneous growth of ceria on porous metal nanoparticles followed by the calcination-induced shrinkage of the nan...We describe a route to the preparation of(metal yolk)/(porous ceria shell)nanostructures through the heterogeneous growth of ceria on porous metal nanoparticles followed by the calcination-induced shrinkage of the nanoparticles.The approach allows for the control of the ceria shell thickness,the metal yolk composition and size,which is difficult to realize through common templating approaches.The yolk/shell nanostructures with monometallic Pt and bimetallic PtAg yolks featuring plasmon-induced broadband light absorption in the visible region are rationally designed and constructed.The superior photocatalytic activities of the obtained nanostructures are demonstrated by the selective oxidation of benzyl alcohol under visible light.The excellent activities are ascribed to the synergistic effects of the metal yolk and the ceria shell on the light absorption,electron-hole separation and efficient mass transfer.Our synthesis of the(metal yolk)/(porous ceria shell)nanostructures points out a way to the creation of sophisticated heteronanostructures for high-performance photocatalysis.展开更多
文摘Articular cartilage plays an important role in weight-bearing and movement.However,it is also more easily damaged in intra-articular fractures.As the cartilage has no vessel and nerve,so it is hardly to repair itself once damaged seriously.Tissue engineering is a potential way to cure the cartilage damage because it combines scaffold and cells.Gels
基金the National Natural Science Foundation of China(Nos.81971734,32071323,and 32271410)Program for Innovative Research Team in Science and Technology in Fujian Province University,Instrumental Analysis Center of Huaqiao University for TEM images,and Subsidized Project for Cultivating Postgraduates’Innovative Ability in Scientific Research of Huaqiao University are gratefully acknowledged。
文摘Nanomaterials doped with non-metallic C have attracted tremendous attention as potential nano-artificial enzymes due to their ability to change the energy band structure to improve their intrinsic properties.Herein,we report a green,facile,efficient,fast strategy to access high-performance nanozymes via supercritical CO_(2)fluid technology-fabricated polymer nanoreactor of poly-(methyl vinyl ether-co-maleic anhydride)(PVM/MA)coated Co(NO_(3))_(2)into C-doped Co_(3)O_(4)(C-Co_(3)O_(4))nanozyme by a onestep calcination process.Converting PVM/MA to C doping into Co_(3)O_(4)shortens the entire lattice constant of the crystal structure,and the overall valence band energy level below the Fermi level shifts toward the lower energy direction.The as-prepared CCo_(3)O_(4)demonstrated significant peroxidase-like catalytic activity,significantly greater than the undoped Co_(3)O_(4)nanoparticle nanozyme.The following density functional theory(DFT)calculations revealed that the doped nano-enzyme catalytic site displayed a unique electronic structure,altering the material surface with more electrons to fill the anti-bond of the two molecular orbitals,significantly improving the peroxidase-like enzyme catalytic and glucose sensor performance.The resultant enzymatic glucose sensing in a linear range of 0.1–0.6 mM with a detection limit of 3.86μM is in line with standard Michaelis–Menten theory.Collectively,this work demonstrates that converting polymers into nanozymes of C-doped form by supercritical CO_(2)fluid technology in a step is an effective strategy for constructing high-performance glucose sensor nanozymes.This cost-effective,reliable,precise system offers the potential for rapid analyte detection,facilitating its application in a variety of fields.
基金supported by the National Natural Science Foundation of China (U1605225, 31570974, and 31470927)the Public Science and Technology Research Funds Projects of Ocean (201505029)+1 种基金the Promotion Program for Young and Middle-aged Teacher in Science and Technology Research of Huaqiao University (ZQN-PY107)the Program for Innovative Research Team in Science and Technology in Fujian Province University
文摘Recently tremendous progress has been evidenced by the advancements in developing innovative three-dimensional(3 D)scaffolds using various techniques for addressing the autogenous grafting of bone. In this work, we demonstrated the fabrication of porous polycaprolactone(PCL) scaffolds for osteogenic differentiation based on supercritical fluid-assisted hybrid processes of phase inversion and foaming. This eco-friendly process resulted in the highly porous biomimetic scaffolds with open and interconnected architectures. Initially, a 2^3 factorial experiment was designed for investigating the relative significance of various processing parameters and achieving better control over the porosity as well as the compressive mechanical properties of the scaffold. Then, single factor experiment was carried out to understand the effects of various processing parameters on the morphology of scaffolds. On the other hand, we encapsulated a growth factor, i.e., bone morphogenic protein-2(BMP-2), as a model protein in these porous scaffolds for evaluating their osteogenic differentiation. In vitro investigations of growth factor loaded PCL scaffolds using bone marrow stromal cells(BMSCs) have shown that these growth factor-encumbered scaffolds were capable of differentiating the cells over the control experiments. Furthermore, the osteogenic differentiation was confirmed by measuring the cell proliferation, and alkaline phosphatase(ALP) activity, which were significantly higher demonstrating the active bone growth. Together, these results have suggested that the fabrication of growth factor-loaded porous scaffolds prepared by the eco-friendly hybrid processing efficiently promoted the osteogenic differentiation and may have a significant potential in bone tissue engineering.
基金support from the National Natural Science Foundation of China(NSFC,32071323,81971734 and 31800794)National Key R&D Program of China(2018YFB1105600)+3 种基金Natural Science Foundation of Fujian Province(2019J01076)support by the Fundamental Research Funds for the Central Universities(ZQN-713)Funds for Foreign Experts from Ministry of Science and Technology,China(G20190013023)Program for Innovative Research Team in Science and Technology in Fujian Province.
文摘Recently,hydrogels have gained enormous interest in three-dimensional(3D)bioprinting toward developing functional substitutes for tissue remolding.However,it is highly challenging to transmit electrical signals to cells due to the limited electrical conductivity of the bioprinted hydrogels.Herein,we demonstrate the 3D bioprinting-assisted fabrication of a conductive hydrogel scaffold based on poly-3,4-ethylene dioxythiophene(PEDOT)nanoparticles(NPs)deposited in gelatin methacryloyl(GelMA)for enhanced myogenic differentiation of mouse myoblasts(C2C12 cells).Initially,PEDOT NPs are dispersed in the hydrogel uniformly to enhance the conductive property of the hydrogel scaffold.Notably,the incorporated PEDOT NPs showed minimal influence on the printing ability of GelMA.Then,C2C12 cells are successfully encapsulated within GelMA/PEDOT conductive hydrogels using 3D extrusion bioprinting.Furthermore,the proliferation,migration and differentiation efficacies of C2C12 cells in the highly conductive GelMA/PEDOT composite scaffolds are demonstrated using various in vitro investigations of live/dead staining,F-actin staining,desmin and myogenin immunofluorescence staining.Finally,the effects of electrical signals on the stimulation of the scaffolds are investigated toward the myogenic differentiation of C2C12 cells and the formation of myotubes in vitro.Collectively,our findings demonstrate that the fabrication of the conductive hydrogels provides a feasible approach for the encapsulation of cells and the regeneration of the muscle tissue.
基金supported by the National Key Research&Development Program of China(2019YFE0113600)the National Natural Science Foundation of China(NSFC 81971734,32071323)and Program for Innovative Research Team in Science,Scientific Research Funds of Huaqiao University(21BS113).
文摘Despite the success,it is highly challenging to battle against pathogenic biofilms-based chronic bacterial infections by conventional antibiotic therapy.Herein,we report a near-infrared(NIR)/acid-induced nanoplatform based on chitosan(CS)-coated indocyanine green(ICG,photosensitizer)/luteolin(LUT,a natural quorum sensing inhibitor)nanocomposites(ICG/LUT-CS)as antibacterial and antibiofilm agents for skin wound healing.Initially,the ICG/LUT nanoplatforms are prepared by the supercritical antisolvent technology and coated with the CS layer.The obtained ICG/LUT-CS with ultra-high encapsulation efficiency exhibited more favorable photothermal conversion effects and improved NIR laser/acid dual-induced drug release behavior than individual modalities,achieving exceptional bacteria-killing and biofilm elimination effects.Moreover,the ICG/LUT-CS realized the synergetic effects of chemotherapy and photothermal therapy outcomes for wound healing.Together,our findings provided an appealing strategy for the rapid preparation and future translational application of ICG/LUT-CS as an ideal agent for fighting against biofilm infections.
基金This work was supported by the National Natural Science Foundation of China(No.61605050)the Natural Science Foundation of Fujian Province of China(Nos.2017J01085,2019J06019)+2 种基金the Promotion Program for Young and Middle-aged Teacher in Science and Technology Research of Huaqiao University(No.ZQN-PY416)the Scientific Research Funds of Huaqiao University(No.16135102)the Program for Innovative Research Team in Science and Technology in Fujian Province University.
文摘We describe a route to the preparation of(metal yolk)/(porous ceria shell)nanostructures through the heterogeneous growth of ceria on porous metal nanoparticles followed by the calcination-induced shrinkage of the nanoparticles.The approach allows for the control of the ceria shell thickness,the metal yolk composition and size,which is difficult to realize through common templating approaches.The yolk/shell nanostructures with monometallic Pt and bimetallic PtAg yolks featuring plasmon-induced broadband light absorption in the visible region are rationally designed and constructed.The superior photocatalytic activities of the obtained nanostructures are demonstrated by the selective oxidation of benzyl alcohol under visible light.The excellent activities are ascribed to the synergistic effects of the metal yolk and the ceria shell on the light absorption,electron-hole separation and efficient mass transfer.Our synthesis of the(metal yolk)/(porous ceria shell)nanostructures points out a way to the creation of sophisticated heteronanostructures for high-performance photocatalysis.