Since potato cultivars are sensitive to low temperature,cold injury severely affects the geographical distribution and yield of potato.Although some miRNAs have been identified in response to cold stress in plants,the...Since potato cultivars are sensitive to low temperature,cold injury severely affects the geographical distribution and yield of potato.Although some miRNAs have been identified in response to cold stress in plants,there is no report about the role of miRNAs in the response to cold stress in potato.Here,via high throughput sequencing,we described the profiling of cold stress response to miRNA and mRNA in potato.Two small RNA and six mRNA libraries were constructed and sequenced.296 known and 211 novel miRNAs were identified,in which 34 miRNAs in Cold Group(CG)had the higher expression quantity than which in Normal Group(NG)and 32 in CG had lower expression quantity than which in NG.3068 differentially expressed genes were detected between NG and CG,in which 1400 genes were up-regulated and 1668 genes were down-regulated.The metabolism pathway of starch and sucrose(ko00500)is the common KEGG pathway in differentially expressed miRNA and mRNA.In this pathway,StuPME21575 and StuPME42971 are pectinesterase which mainly catalyzes the pectin-forming pectate,which are controlled by stu-miR6023 and stu-novel-miR42365.As the potato suffering cold stress,these two miRNAs expression levels became higher,but their target genes expression levels were just opposite and this result is the same with qRT-PCR.展开更多
基金This work was supported by grants from the Earmarked Fund for Modern Agro-Industry Technology Research System of China(CARS-09-ES10)National Key Research and Development Project‘Integrated Research and Demonstration of Potato Fertilizer and Pesticide Reduction Technology(2018YFD0200800)’+2 种基金Anhui Academy of Agricultural Sciences Dean’s Youth Fund(17B0306)Anhui Academy of Agricultural Sciences‘New Dr.’scientific research project,Anhui Science and Technology Major Project‘Breeding of New Strawberry Varieties in Facilities and Integration and Demonstration of Cultivation Techniques for Improving Quality and Efficiency’(201903a06020004)Potato Taro Breeding and Cultivation Team Project(2020YL029).
文摘Since potato cultivars are sensitive to low temperature,cold injury severely affects the geographical distribution and yield of potato.Although some miRNAs have been identified in response to cold stress in plants,there is no report about the role of miRNAs in the response to cold stress in potato.Here,via high throughput sequencing,we described the profiling of cold stress response to miRNA and mRNA in potato.Two small RNA and six mRNA libraries were constructed and sequenced.296 known and 211 novel miRNAs were identified,in which 34 miRNAs in Cold Group(CG)had the higher expression quantity than which in Normal Group(NG)and 32 in CG had lower expression quantity than which in NG.3068 differentially expressed genes were detected between NG and CG,in which 1400 genes were up-regulated and 1668 genes were down-regulated.The metabolism pathway of starch and sucrose(ko00500)is the common KEGG pathway in differentially expressed miRNA and mRNA.In this pathway,StuPME21575 and StuPME42971 are pectinesterase which mainly catalyzes the pectin-forming pectate,which are controlled by stu-miR6023 and stu-novel-miR42365.As the potato suffering cold stress,these two miRNAs expression levels became higher,but their target genes expression levels were just opposite and this result is the same with qRT-PCR.
