[ Objective] The research aimed to discuss the differences of the expression amount of the related gene that regulated the development of muscles under different feeding environments. [ Method ] Using fluorescence qua...[ Objective] The research aimed to discuss the differences of the expression amount of the related gene that regulated the development of muscles under different feeding environments. [ Method ] Using fluorescence quantitative PCR, the effects of fermentation bed cultivation model on the expression mount of MSTN in pig gluteus were studied and the correlation between its expression amount and the carcass quality of pig was analyzed. [ Result] The relative expression amount of MSTN mRNA of swine gluteus under fermentation bed cultivation model decreased 8.6% by that under conventional cement ground feeding model, but there was no significant difference(P 〉0.05). The expression amount of MSTN mRNA in pig gluteus undo" conventional cement ground feeding model showed a significant negative correlation with shear force ( P 〈 0.05). The expression amount of MSTN mRNA in pig gluteus under fermentation bed cultivation model showed significant negative correlations with water content and muscle fiber diameter(P 〈 0.05 ). The expression amount of MSTN mRNA in pig gluteus under two cultivation models had no significant correlation with other meat quality indices(P 〉 0.05). [ Conclusion] The research could provide test materials for the studies on the regulation of pork quality.展开更多
基金Supported by Agricultural Independent Innovation Fund in Jiangsu Province[CX12(1001)]
文摘[ Objective] The research aimed to discuss the differences of the expression amount of the related gene that regulated the development of muscles under different feeding environments. [ Method ] Using fluorescence quantitative PCR, the effects of fermentation bed cultivation model on the expression mount of MSTN in pig gluteus were studied and the correlation between its expression amount and the carcass quality of pig was analyzed. [ Result] The relative expression amount of MSTN mRNA of swine gluteus under fermentation bed cultivation model decreased 8.6% by that under conventional cement ground feeding model, but there was no significant difference(P 〉0.05). The expression amount of MSTN mRNA in pig gluteus undo" conventional cement ground feeding model showed a significant negative correlation with shear force ( P 〈 0.05). The expression amount of MSTN mRNA in pig gluteus under fermentation bed cultivation model showed significant negative correlations with water content and muscle fiber diameter(P 〈 0.05 ). The expression amount of MSTN mRNA in pig gluteus under two cultivation models had no significant correlation with other meat quality indices(P 〉 0.05). [ Conclusion] The research could provide test materials for the studies on the regulation of pork quality.