[Objectives]This study aimed at providing gist for selecting qualified transport containers by comparing the performance of common SPF mouse transport containers at home and abroad,and laying a foundation for subseque...[Objectives]This study aimed at providing gist for selecting qualified transport containers by comparing the performance of common SPF mouse transport containers at home and abroad,and laying a foundation for subsequent improvement of transport containers.[Methods]In this study,5 different transport containers were selected,and their microbial filtration performance,ventilation,sterilization method and flux,and other related design and construction were analyzed.[Results]The results showed all 5 SPF mouse transport containers can provide enough air for the survival of mouse and protect them from bacterial infections.Transport container B and D are superior to others on one-time sterilization flux and deformation degree after autoclaving.Furthermore,taking into account the related design and construction of these transport containers,such as viewing window and carrying handle,the result of comprehensive comparison is that the transport container D>A>E>C>B.[Conclusions]In summary,the 5 SPF transport containers tested in this study can all satisfy the basic demand of SPF mouse transportation,but each transport container has its own advantages and disadvantages on storage space,deformation degree after autoclaving and other aspects.Thus,SPF mouse transport containers should be selected by different demand.展开更多
[Objectives]This study was conducted to select suitable mouse beddings from commercial corncob bedding(A-E)produced by five different manufacturers.[Methods]The physical properties and microbial indexes of the five ki...[Objectives]This study was conducted to select suitable mouse beddings from commercial corncob bedding(A-E)produced by five different manufacturers.[Methods]The physical properties and microbial indexes of the five kinds of corncob bedding were studied by means of visual inspection,dust content determination,moisture content determination,water absorption tests and microbial detection.[Results]In visual inspection,corncob bedding A was better than other four kinds.During dust content determination,corncob bedding D was higher than other 4 kinds(1.83%±0.2983%,P<0.05).In terms of moisture content,the moisture contents of the five kinds of corncob bedding were less than or equal to 10%.In the water absorption test,the water absorption capacities of the five kinds of bedding before autoclaving were all greater than 150%,and the water absorption of corncob bedding D after autoclaving was better than other four kinds(171.0±5.3,P<0.05).During microbial detection,the total number of colonies of corncob bedding B before autoclaving was higher than those of other four types(1.42×10^(5)±4.3×10^(4),P<0.05).The five kinds of commercial corncob bedding have different physical parameters and microbial conditions,and each has its own advantages and disadvantages.When purchasing and selecting corncob bedding,it is necessary to comprehensively consider the needs of daily feeding work,and choose bedding according to the effects on the health and welfare of experimental animals.[Conclusions]This study provides a reference for selecting suitable corncob bedding for mouse.展开更多
基金Supported by Scientific Research Project of Jiangsu Laboratory Animal Association(DWXH201907).
文摘[Objectives]This study aimed at providing gist for selecting qualified transport containers by comparing the performance of common SPF mouse transport containers at home and abroad,and laying a foundation for subsequent improvement of transport containers.[Methods]In this study,5 different transport containers were selected,and their microbial filtration performance,ventilation,sterilization method and flux,and other related design and construction were analyzed.[Results]The results showed all 5 SPF mouse transport containers can provide enough air for the survival of mouse and protect them from bacterial infections.Transport container B and D are superior to others on one-time sterilization flux and deformation degree after autoclaving.Furthermore,taking into account the related design and construction of these transport containers,such as viewing window and carrying handle,the result of comprehensive comparison is that the transport container D>A>E>C>B.[Conclusions]In summary,the 5 SPF transport containers tested in this study can all satisfy the basic demand of SPF mouse transportation,but each transport container has its own advantages and disadvantages on storage space,deformation degree after autoclaving and other aspects.Thus,SPF mouse transport containers should be selected by different demand.
文摘[Objectives]This study was conducted to select suitable mouse beddings from commercial corncob bedding(A-E)produced by five different manufacturers.[Methods]The physical properties and microbial indexes of the five kinds of corncob bedding were studied by means of visual inspection,dust content determination,moisture content determination,water absorption tests and microbial detection.[Results]In visual inspection,corncob bedding A was better than other four kinds.During dust content determination,corncob bedding D was higher than other 4 kinds(1.83%±0.2983%,P<0.05).In terms of moisture content,the moisture contents of the five kinds of corncob bedding were less than or equal to 10%.In the water absorption test,the water absorption capacities of the five kinds of bedding before autoclaving were all greater than 150%,and the water absorption of corncob bedding D after autoclaving was better than other four kinds(171.0±5.3,P<0.05).During microbial detection,the total number of colonies of corncob bedding B before autoclaving was higher than those of other four types(1.42×10^(5)±4.3×10^(4),P<0.05).The five kinds of commercial corncob bedding have different physical parameters and microbial conditions,and each has its own advantages and disadvantages.When purchasing and selecting corncob bedding,it is necessary to comprehensively consider the needs of daily feeding work,and choose bedding according to the effects on the health and welfare of experimental animals.[Conclusions]This study provides a reference for selecting suitable corncob bedding for mouse.
