In the last decades, the use of in vitro systems in liver research has grown exponentially. Important reasons promoting this work are the high throughput and ease of genetic manipulations afforded by these experiments...In the last decades, the use of in vitro systems in liver research has grown exponentially. Important reasons promoting this work are the high throughput and ease of genetic manipulations afforded by these experiments relative to in vivo experiments. Thousands of investigations of hepatocellular carcinoma have been performed employing the human hepatoma Huh-7 cell line. The extensive body of knowledge produced attests to the importance and value of this in vitro cell system to study the characteristics of hepatomas and the potential of natural and synthetic compounds to prevent and eliminate this liver cancer. The necessarily brief summary provided here attempts to summarise some of the most recent achievements and limitations of investigations with Huh-7 cells and derivatives.展开更多
Herbicide tolerant plants such as Roundup-Ready soybean contain residues of glyphosate herbicide. These residues are considered safe and previous animal-feeding-studies have failed to find negative effects related to ...Herbicide tolerant plants such as Roundup-Ready soybean contain residues of glyphosate herbicide. These residues are considered safe and previous animal-feeding-studies have failed to find negative effects related to such chemical residues. The present study tests 8 experimental soy- meal diets as feed in groups (each containing 20 individuals) of test-animals (D. magna). The diets have different levels of glyphosate residues and we show that animal growth, reproductive maturity and number of offspring are correlated with these chemicals. The tested soybeans are from ordinary agriculture in Iowa USA and the residues are below the regulatory limits. Despite this, clear negative effects are seen in life-long feeding. The work enhances the need for including analysis of herbicide residues in future assessment of GMO.展开更多
文摘In the last decades, the use of in vitro systems in liver research has grown exponentially. Important reasons promoting this work are the high throughput and ease of genetic manipulations afforded by these experiments relative to in vivo experiments. Thousands of investigations of hepatocellular carcinoma have been performed employing the human hepatoma Huh-7 cell line. The extensive body of knowledge produced attests to the importance and value of this in vitro cell system to study the characteristics of hepatomas and the potential of natural and synthetic compounds to prevent and eliminate this liver cancer. The necessarily brief summary provided here attempts to summarise some of the most recent achievements and limitations of investigations with Huh-7 cells and derivatives.
文摘Herbicide tolerant plants such as Roundup-Ready soybean contain residues of glyphosate herbicide. These residues are considered safe and previous animal-feeding-studies have failed to find negative effects related to such chemical residues. The present study tests 8 experimental soy- meal diets as feed in groups (each containing 20 individuals) of test-animals (D. magna). The diets have different levels of glyphosate residues and we show that animal growth, reproductive maturity and number of offspring are correlated with these chemicals. The tested soybeans are from ordinary agriculture in Iowa USA and the residues are below the regulatory limits. Despite this, clear negative effects are seen in life-long feeding. The work enhances the need for including analysis of herbicide residues in future assessment of GMO.
