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Identification and Validation of Single Nucleotide Polymorphisms in Poplar Using Publicly Expressed Sequence Tags 被引量:3
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作者 Bo ZHANG Yan ZHOU +3 位作者 Liang ZHANG Qiang ZHUGE Ming-Xiu WANG Min-Ren HUANG 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第12期1493-1499,共7页
By using assembled expressed sequence tags (ESTs) from 14 different eDNA libraries that contain 84 132 sequences reads, 556 Populus candidate single nucleotide polymorphisms (SNPs) were identified. Because traces ... By using assembled expressed sequence tags (ESTs) from 14 different eDNA libraries that contain 84 132 sequences reads, 556 Populus candidate single nucleotide polymorphisms (SNPs) were identified. Because traces were not available from dbEST (http://www.ncbi.nlm.nih.gov/dbEST/index.html), stringent filters were used to identify reliable candidate SNPs. Sequences analysis indicated that the main types of substitutions among candidate SNPs were A/G and T/C transitions, which accounted for 22.0% and 30.8%, respectively. One hundred and ten candidate SNPs were tested. As a result, 38 candidate SNPs were confirmed by directed sequencing of PCR products amplified from six different individuals. Thirteen new SNPs in intron regions were found and multiple SNPs were found to be located in both intron and exon regions of four contigs. Heterozygosis was found in all 47 candidate sites and five SNP sites were heterozygous in all six samples. This is the first report of SNP identification in a tree species which reveals that assembled ESTs from multiple libraries of the public database may provide a rich source of comparative sequences for an SNP search in the poplar genome. 展开更多
关键词 expressed sequence tag POPLAR single nucleotide polymorphism.
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PNAS:科学焉已在蝙蝠机体中发现白血病病毒的史前祖先
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作者 Helena Farkašová Tomáš Hron +4 位作者 Jan Pačes Pavel Hulva Petr Benda Robert James Gifford Daniel Elleder 《现代生物医学进展》 CAS 2017年第11期I0002-I0002,共1页
近日,一项刊登在国际杂志PNAS上的研究报告中,来自格拉斯哥大学和捷克科学院的研究人员通过研究在蝙蝠的基因组中发现了引发罕见类型白血病的病毒家族的古老DNA痕迹。
关键词 白血病病毒 科学院 蝙蝠 祖先 机体 研究人员 DNA 基因组
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