Objective:To study carbon and nitrogen utilization pattern and arthrospore formation in a psychrotolerant yeast isolate Rhodotorula mucilaginosa(R.mucilaginosa)BPT1.Methods:Growth of the yeast on minimal synthetic med...Objective:To study carbon and nitrogen utilization pattern and arthrospore formation in a psychrotolerant yeast isolate Rhodotorula mucilaginosa(R.mucilaginosa)BPT1.Methods:Growth of the yeast on minimal synthetic medium supplemented with various carbon and nitrogen compounds as sole carbon or nitrogen source has been studied.Various physico-chemical parameters such as pH,restricted oxygen supply,temperatures,media composition and presence of methionine were tested to examine their effect on arthrospore formation by this known opportunistic pathogen.Results:The psychrotolerant isolate BPT1 identified on the basis of D1/D2 domain of large rDNA sequence characteristics as R.mucilaginosa showed some deviation in carbon and nitrogen utilization patterns from those of other strains of R.mucilaginosa in the CBS data base.Intriguingly,the isolate produced sub-surface hyphal rays around its colony at lower temperatures(4℃and 20℃)on PDA medium;the ray was found to be linearly arranged arthrospores.The arthrospore was not produced in liquid medium,or in presence of methionine or under micro-aerobic condition or at higher temperature.Conclusions:The investigation showed a novel feature i.e.arthrospore was formed by this yeast isolate under specific set of conditions.The results reiterated that only physiological and morphological characteristics were not sufficient to identify a yeast.The ability of R.mucilaginosa to form arthrospores seems to be an adaptive feature in response to extreme environmental condition,and represents adaptive ability having something to do with its ubiquity.展开更多
基金supported by Madhya Pradesh Council of Science and Technology,Bhopal(MP)(Grant No.3902/CST/R&D/2011).
文摘Objective:To study carbon and nitrogen utilization pattern and arthrospore formation in a psychrotolerant yeast isolate Rhodotorula mucilaginosa(R.mucilaginosa)BPT1.Methods:Growth of the yeast on minimal synthetic medium supplemented with various carbon and nitrogen compounds as sole carbon or nitrogen source has been studied.Various physico-chemical parameters such as pH,restricted oxygen supply,temperatures,media composition and presence of methionine were tested to examine their effect on arthrospore formation by this known opportunistic pathogen.Results:The psychrotolerant isolate BPT1 identified on the basis of D1/D2 domain of large rDNA sequence characteristics as R.mucilaginosa showed some deviation in carbon and nitrogen utilization patterns from those of other strains of R.mucilaginosa in the CBS data base.Intriguingly,the isolate produced sub-surface hyphal rays around its colony at lower temperatures(4℃and 20℃)on PDA medium;the ray was found to be linearly arranged arthrospores.The arthrospore was not produced in liquid medium,or in presence of methionine or under micro-aerobic condition or at higher temperature.Conclusions:The investigation showed a novel feature i.e.arthrospore was formed by this yeast isolate under specific set of conditions.The results reiterated that only physiological and morphological characteristics were not sufficient to identify a yeast.The ability of R.mucilaginosa to form arthrospores seems to be an adaptive feature in response to extreme environmental condition,and represents adaptive ability having something to do with its ubiquity.
