In vitro scolicidal effect of Satureja khuzistanica(Jamzad)essential oil

Objective:To investigate the scolicidal effect of the Satureja khuzistanica(S.khusistanica) essential oil from aerial parts of this herbal plant.Methods:The essential oil was obtained by hydrodistillation method.Gas chromatography(GC) and gas chromatography mass spectrometry(GC-MS) were employed to determine the chemical composition of the essential oil.Protoscolices were collected aseptically from sheep livers containing hydatid cyst.Protoscolices were exposed to various concentrations of the oil(3,5 and 10 mg/mL) for 10,20,30,and 60 min.Viability of protoscolices was confirmed by 0.1%eosin staining.Results:A total of 19 compounds representing 97.6%of the total oil,were identified.Carvacrol(94.9%) was found to be the major essential oil constituent.Scolicidal activity of S.khuzistanica essential oil at concentration of 3 mg/mL was 28.58,32.71,37.20 and 42.02%,respectively.This essential oil at concentration of 5 mg/ mL killed 51.33,66.68,81.12,and 100%.of protoscolices after 10,20,30 and 60 min,respectively. One hundred scolicidal effect was observed with S.khuzistanica essenlial oil at the concentration of 10 mg/mL after 10 min(comparing with 7.19%for control group).Conclusions:The essenlial oil of S.khuzistanica is rich in carvacrol and may be used as a natural scolicidal agent.

The Effects of a Novel Multicomponent Transition Metal Dichalcogenide on Nervous System Regeneration

In regenerative medicine,a scaffold is needed to provide physical support for the growth of cells at the injury site.Carbon composites are also widely used in biomedicine.This research aimed to see if(MoWCu)S/rGO could be used in peripheral and central neural regeneration as a carbon-based nanomaterial.This material was created using a one-step hydrothermal process.We used Scanning Electron Microscopy with Energy Dispersive X-ray analysis(SEM–EDX),X-ray diffraction,and Field-Emission Scanning Electron Microscopy(FE-SEM)to describe it.The researchers used animal models of spinal cord injury and sciatic nerve injury to assess its effect as a scaffold of anti-inflammatory and electrical conductivity.The Basso Beattie Bresnahan locomotor rating scale and von Frey Filament were used to assess neuronal function after(MoWCu)S/rGO transplantation.In addition,the expression of p75 NTR and neurotrophic factors(BDNF,NT3,and NGF)mRNA in the experimental rats nerve was compared to the normal ones using Real-Time RT-qPCR.In the experimental groups,the use of(MoWCu)S/rGO resulted in a significant increase in neurotrophic factor gene expression,while p75 NTR was inversely decreased.In conclusion,we found that the nerve regeneration activity of the(MoWCu)S/rGO scaffold in rat models significantly increased motor function recovery in the treated groups.Furthermore,the current study explained the response of this composite to inflammatory neurodegenerative diseases.(MoWCu)S incorporation in graphene is thought to have excellent properties and may be used in regenerative medicine.